A5063654523- Clinical Laboratory Practices and Quality Control
- Protein Structure and Dynamics
- Hormonal and reproductive studies
- Chronic Kidney Disease and Diabetes
- Pharmacological Effects and Assays
- Bacterial Genetics and Biotechnology
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Dialysis and Renal Disease Management
- Risk and Safety Analysis
- Muscle metabolism and nutrition
- Molecular spectroscopy and chirality
- Renal Diseases and Glomerulopathies
- Healthcare cost, quality, practices
- RNA Interference and Gene Delivery
- Estrogen and related hormone effects
- DNA and Nucleic Acid Chemistry
- Meta-analysis and systematic reviews
- Lipoproteins and Cardiovascular Health
- Pharmaceutical studies and practices
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Meteorological Phenomena and Simulations
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Electrowetting and Microfluidic Technologies
- Fluid Dynamics and Turbulent Flows
University Hospitals Birmingham NHS Foundation Trust
2020-2025
Healthcare Quality Improvement Partnership
2019
Heart of England NHS Foundation Trust
2010-2012
Heartlands Hospital
2010-2011
University of Warwick
2002-2007
Single‐stranded guanosine‐rich oligodeoxyribonucleotides (GROs) have a propensity to form quadruplex structures that are stabilized by G‐quartets. In addition intense speculation about the role of G‐quartet formation in vivo, there is considerable interest therapeutic potential oligonucleotides as aptamers or non‐antisense antiproliferative agents. We previously described several GROs inhibit proliferation and induce apoptosis cancer cell lines. The activity these was related their ability...
Long molecules such as fibrous proteins are particularly difficult to characterise structurally. We have recently designed a microvolume Couette flow linear dichroism (LD) cell whose sample volume is only 20–40 µL in contrast previous cells where the of required has typically been order 1000–2000 µL. This brings requirements LD level it can be used for biological samples. Since difference absorption light polarised parallel an orientation direction and perpendicular that direction, ideal...
The first page of this article is displayed as the abstract.
As one of the most requested profiles blood tests, there is a need for standardization among liver function tests (LFT). Alanine aminotransferase (ALT) and aspartate (AST) are key markers hepatocellular injury. ALT AST used to calculate Fibrosis-4 (FIB-4) score assessing fibrosis. Despite recommendations by International Federation Clinical Chemistry (IFCC) include pyridoxal-5-phosphate in assay methodologies, laboratories continue omit this.
Polymer formation by the essential FtsZ protein plays a crucial role in cytokinesis of most prokaryotes. Lateral associations between these polymers to form bundles or sheets are widely predicted be extremely important for function vivo. We have carried out study vitro polymer and bundling using linear dichroism (LD) assess structural properties polymers. demonstrate proof-of-principle experiments show that LD can used as technique follow polymerization, we present spectra Our subsequent...
ABSTRACT Context Androgen excess is common in women and refers to clinical or biochemical evidence of elevated androgenic steroids such as testosterone. It associated with underlying polycystic ovary syndrome the majority cases. However severe androgen less may indicate presence adrenal ovarian neoplasms, genetic disorders insulin resistance syndromes. Currently there are few consensus guidelines assist clinicians a standardised management approach patient excess. Design Clinical practice...
Abstract Objectives Coagulation test results may be affected by hemolysis, icterus and/or lipemia (HIL). Detailed guidelines for HIL-management are missing, both manual and automatic HIL-checks. The aim of this survey was to provide an overview the practical procedures detection handling HIL-samples used laboratories in Europe context coagulation testing. Methods A SurveyMonkey questionnaire sent from Haemostasis Working Group European Organisation External Quality Assurance Providers...
Introduction A number of guidance documents have been published in recent years for the diagnosis and management hypogonadism (HG). Laboratory practice has a major role supporting guidelines with accurate precise serum total testosterone (TT) methods standardised pre- post-analytical protocols. Our study investigated whether laboratory currently supports HG. Methods An internet-based questionnaire survey senior biochemists (UK/Republic Ireland) was conducted (April-May 2018). Questions...
The Tat system catalyzes the transport of folded globular proteins across bacterial plasma membrane and chloroplast thylakoid. It recognizes cleavable signal peptides containing a critical twin‐arginine motif but little is known overall structure these peptides. In this report, we have analyzed secondary SufI peptide, together with those two nonfunctional variants in which region around twin‐arginine, RRQFI, replaced by KKQFI or RRQAA. Circular dichroism studies show that peptide exists as...
Abstract A thermostatted micro volume Couette cell has been designed to enable linear dichroism ( LD ) data be collected at a range of temperatures. The is development the traditional flow and includes recent micro‐volume (20–40 μL) coupled with addition heating element, temperature probe controller. This new opens way not only analysis systems where sample critical, but also for sensitive samples. polymerization microtubule protein tubulin followed in different conditions using cell....
Background: Inter-assay variation between different immunoassays and mass spectrometry methods hampers the biochemical confirmation of male hypogonadism. Furthermore, some laboratories utilis eassay manufacturer reference ranges that do not necessarily mirror assay performance characteristics, with lower limit normality ranging from 4.9 nmol/L to 11 nmol/L. The quality normative data underlying commercial immunoassay is uncertain.Design: A working group reviewed published evidence agreed...
Abstract Objectives Laboratories need to take into consideration the specificity and imprecision of assays not only in verification, but also quality assessment. This study investigates composition serum used EQA materials by comparing material from a single multiple donors (pooled material), across methods, using creatinine as an example. Methods Sixteen different matrices were distributed 36 specimens through UK NEQAS for Acute Chronic Kidney Disease Scheme March 2022 2023. Male-only...