A5034048649- Influenza Virus Research Studies
- Clinical Laboratory Practices and Quality Control
- Respiratory viral infections research
- Immune Response and Inflammation
- Viral Infections and Vectors
- interferon and immune responses
- Healthcare cost, quality, practices
- SARS-CoV-2 and COVID-19 Research
- Bacterial Identification and Susceptibility Testing
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Meta-analysis and systematic reviews
- Health and Medical Research Impacts
- Viral Infections and Outbreaks Research
- Ethics in Clinical Research
- Quality and Safety in Healthcare
- Risk and Safety Analysis
- COVID-19 epidemiological studies
- Global Health and Surgery
- Biomedical and Engineering Education
- SARS-CoV-2 detection and testing
- COVID-19 Pandemic Impacts
- Biosensors and Analytical Detection
- Scientific Computing and Data Management
- COVID-19 impact on air quality
University of British Columbia
2023-2025
University of Washington
2014-2024
Washington State Department of Health
2023
Office of the National Coordinator for Health Information Technology
2021-2022
Seattle University
2021
TRIA Bioscience (United States)
2012-2014
National Center for Immunization and Respiratory Diseases
2007-2013
Centers for Disease Control and Prevention
2007-2010
The University of Texas Medical Branch at Galveston
2005-2006
Howard Hughes Medical Institute
2005
Fatal human respiratory disease associated with the 1918 pandemic influenza virus and potentially H5N1 viruses is characterized by severe lung pathology, including pulmonary edema extensive inflammatory infiltrate. Here, we quantified cellular immune response to infection in mouse flow cytometry demonstrate that mice infected highly pathogenic (HP) H1N1 exhibit significantly high numbers of macrophages neutrophils lungs compared low (LP) viruses. Mice a recent isolate show considerable...
The proapoptotic PB1-F2 protein of influenza A viruses has been shown to contribute pathogenesis in the mouse model. Expression full-length increases virus, causing weight loss, slower viral clearance, and increased titers lungs. After comparing from Hong Kong 1997 H5N1 outbreak, one amino acid change (N66S) was found sequence at position 66 that correlated with pathogenicity. This same also 1918 pandemic A/Brevig Mission/18 virus. Two isogenic recombinant chimeric were created an A/WSN/33...
The 1918 influenza pandemic was exceptionally severe, resulting in the death of up to 50 million people worldwide. Here, we show which virus genes contributed replication and virulence virus. Recombinant viruses, were replaced with from a contemporary human H1N1 virus, A/Texas/36/91 (Tx/91), generated. exchange most seasonal did not alter virus; however, substitution hemagglutinin (HA), neuraminidase (NA), or polymerase subunit PB1 significantly affected ability this cause severe disease...
ABSTRACT Influenza vaccines capable of inducing cross-reactive or heterotypic immunity could be an important first line prevention against a novel subtype virus. virus-like particles (VLPs) displaying functional viral proteins are effective replication-competent homologous virus, but their ability to induce has not been adequately tested. To measure VLP vaccine efficacy known influenza pandemic recombinant VLPs were generated from structural the 1918 H1N1 Mucosal and traditional parenteral...
Severe acute respiratory syndrome (SARS) caused by a novel human coronavirus (CoV), designated SARS-CoV, is highly contagious disease with the lungs as major target. Although exact mechanism of SARS-CoV pathogenesis remains unknown, an intense, ill-regulated local inflammatory response has been suggested partially responsible for devastating lung pathology. We investigated interaction macrophages (Mphi) and dendritic cells (DC), two key innate immune host system, focusing on their...
Severe acute respiratory syndrome (SARS), caused by a novel coronavirus (CoV) known as SARS-CoV, is contagious and life-threatening illness with pneumocytes its main target. A full understanding of how SARS-CoV would interact lung epithelial cells will be vital for advancing our knowledge SARS pathogenesis. However, an in vitro model infection using relevant not yet available, making it difficult to dissect the pathogenesis lungs. Here, we report that can productively infect human bronchial...
Highly pathogenic avian influenza viruses of the H5N1 subtype continue to cross species barrier infect humans and cause severe disease. It has been suggested that an exaggerated immune response contributes pathogenesis virus infection in mammals. In particular, infections are associated with a high expression proinflammatory cytokines, including interleukin-1 (IL-1) tumor necrosis factor alpha (TNF-α).We investigated compounding affects both cytokines on outcome disease by using triple...
Highly pathogenic influenza A viruses, including avian H5N1 viruses and the 1918 pandemic virus, cause severe respiratory disease in humans animals. Virus infection is followed by intense pulmonary congestion due to an extensive influx of macrophages neutrophils, which can release large quantities reactive oxygen species potentially contributing pathogenesis lung disease. Here, role nitric oxide (NO), a potent signaling molecule inflammation, was evaluated following highly virus challenge...
Extensive preparation is underway to mitigate the next pandemic influenza outbreak. New vaccine technologies intended supplant egg-based production methods are being developed, with recombinant hemagglutinin (rHA) as most advanced program for preventing seasonal and avian H5N1 Influenza. Increased efforts focused on adjuvants that can broaden immunogenicity against emerging viruses maximize supply a worldwide scale. Here, we test protection flu by using H5N1-derived rHA GLA-SE, two-part...
The ongoing threat from Influenza necessitates the development of new vaccine and adjuvant technologies that can maximize immunogenicity, shorten production cycles, increase global supply. Currently, most successful adjuvants for vaccines are squalene-based oil-in-water emulsions. These enhance seroprotective antibody titers to homologous heterologous strains virus, augment a significant dose sparing activity could improve manufacturing capacity. As an alternative emulsion, we tested simple...
To support their replication, viruses take advantage of numerous cellular factors and processes. Recent large-scale screens have identified hundreds such factors, yet little is known about how exploit any these. Influenza virus infection post-translationally activates P58IPK, a inhibitor the interferon-induced, dsRNA-activated eIF2α kinase, PKR. Here, we report that P58IPK knockout mice with influenza resulted in increased lung pathology, immune cell apoptosis, PKR activation, mortality....
Pathology and Laboratory Medicine discipline occupies a special place in the medical school healthcare ecosystem as it bridges basic science clinical practice. In era of rapid knowledge technology evolution, new ways communication, training requirements, demand personalized precision diagnostics, this specialty is facing unprecedented challenges opportunities. Some these are institution-specific, while many shared worldwide at different magnitudes. This review shares our team efforts...
<sec> <title>BACKGROUND</title> Data dashboards are popular tools for supporting routine monitoring and decision-making in public health. Two were developed Côte d’Ivoire to visualize laboratory data on HIV viral load (VL) early infant diagnosis (EID) testing. </sec> <title>OBJECTIVE</title> This study assessed the values, attitudes, experiences regarding data-driven VL EID among existing potential dashboard users d’Ivoire. <title>METHODS</title> We conducted a qualitative including two...
Yeast two-hybrid screens led to the identification of Ubc9 and PIASy, E2 E3 small ubiquitin-like modifier (SUMO)-conjugating enzymes, as proteins interacting with capsid (CA) protein Moloney murine leukemia virus. The binding site in CA for was mapped by deletion alanine-scanning mutagenesis a consensus motif SUMOylation at residues 202 220, PIASy 114 176, directly centered on major homology region. Expression tagged SUMO-1 resulted covalent transfer vivo. Mutations lysine arginines near...
Two strains of Punta Toro virus (PTV), isolated from febrile humans in Panama, cause a differential pathogenesis Syrian hamsters, which could be useful model for understanding the virulence characteristics and outcomes other phleboviral infections such as Rift Valley fever virus. Genetic reassortants produced between lethal Adames (A/A/A) nonlethal Balliet (B/B/B) were used this study to investigate viral genetic determinants lethality hamster model. The S segment was revealed critical...
To implement a mentored laboratory quality stepwise implementation (LQSI) programme to strengthen the and capacity of Cambodian hospital laboratories.
Since 2019, the WHO recommends development and implementation of National Essential Diagnostics List (NEDL) to facilitate availability In-Vitro (IVDs) across various tiers healthcare pyramid, facilities with or without a laboratory on-site. To be effective, NEDL should take into consideration challenges opportunities associated current modalities for organization tier specific testing services in-country. We conducted mixed-methods analysis set out explore available national policies,...