A5090514286- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Nanofabrication and Lithography Techniques
- Receptor Mechanisms and Signaling
- Cell Adhesion Molecules Research
- Alzheimer's disease research and treatments
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Neurobiology and Insect Physiology Research
- RNA modifications and cancer
- Advanced Fluorescence Microscopy Techniques
- Photochromic and Fluorescence Chemistry
- Cholinesterase and Neurodegenerative Diseases
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Chemical Synthesis and Analysis
- Cellular transport and secretion
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Nuclear Receptors and Signaling
- Prion Diseases and Protein Misfolding
Goethe University Frankfurt
2021-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2018
Universidad Nacional de Córdoba
2015-2018
Instituto de Investigación Médica Mercedes y Martín Ferreyra
2015-2018
Cell-cell communication relies on the assembly of receptor-ligand complexes at plasma membrane. The spatiotemporal receptor organization has a pivotal role in evoking cellular responses. We studied clustering heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) and established photoinstructive matrix with ultrasmall lock-and-key interaction pairs to control lateral membrane hormone neuropeptide Y2 living cells by light. Within seconds, was modulated size,...
Alzheimer's disease (AD) is characterized by the deposition of aggregated species amyloid beta (Aβ) in brain, which leads to progressive cognitive deficits and dementia. Aβ generated successive cleavage precursor protein (APP), first β-site APP cleaving enzyme 1 (BACE1) subsequently γ-secretase complex. Those conditions enhace or reduce its clearance predispose aggregation development AD. In vitro studies have demonstrated that assemblies spark a feed-forward loop heightening production....
Extrinsic apoptosis is initiated by recognition and clustering of the single‐pass transmembrane proteins Fas ligand expressed at surface closely apposed lymphocytes target cells, respectively. Since Fas‐mediated death response was mainly studied with soluble antibodies, mobility constraints for receptor activation a membrane embedded agonist not well understood. We explored this influence stimulating on functionalized supported lipid bilayers, where we quantified z‐scan fluorescence...
Membrane receptor clustering is fundamental to cell-cell communication; however, the physiological function of in cell signaling remains enigmatic. Here, we developed a dynamic platform induce cluster formation neuropeptide Y2 hormone receptors (Y2R) situ by chelator nanotool. The multivalent interaction enabled exchange histidine-tagged Y2R within clusters. Fast enrichment clustered areas triggered ligand-independent as determined an increase cytosolic calcium and migration. Notably,...
A relevant question in cell biology with broad implications biomedicine is how the organization and dynamics of interacting membranes modulate signaling cascades that involve cell–cell contact. The functionalization surfaces supported lipid bilayers containing tethered proteins a particularly useful method to present ligands membrane-like mobility cells. Here, we generate micrometer-sized patches decorated proteins. uses an economic microcontact printing technique based on one-photon...
The cytotoxic synapse formed between T lymphocytes or natural killer cells expressing CD 95L and target with 95 on their surface is a key pathway for apoptosis induction by the immune system. Despite similarities in antigen presenting cells, little known about role of spatiotemporal organization agonistic proteins/receptor interactions signaling. Here, we have developed an artificial to examine how mobility geometry anti‐ antibody affect receptor aggregation mobility, ie first step...
T cell receptor (TCR) clustering and formation of an immune synapse are crucial for TCR signaling. However, limited information is available about these dynamic assemblies their connection to transmembrane In this work, controlled via plug-and-play nanotools based on engineered irreversible conjugation pair a peptide-loaded major histocompatibility complex (pMHC) molecule compare assembly in ligand (pMHC)-induced or ligand-independent manner. A streptavidin-binding peptide displayed both...
Abstract Cells sense their environment, receive, and process a diverse set of chemical mechanical signals through transmembrane receptors. How receptors integrate these to orchestrate wide variety physiological outcomes remains one the biggest questions in biology. Here, I discuss crucial aspects receptor assembly highlighting recent tools limitations.
Engineering Mesoscale T Cell Receptors In article number 2310407, Robert Tampé and co-workers introduce innovative nanotools to control cell receptor (TCR) clustering, crucial for immune recognition. By comparing ligand-induced ligand-free conditions, researchers discovered that architecture significantly impacts TCR assembly. This approach enhances our understanding of activation, paving the way advanced immunotherapies, including CAR therapies. Image credit: M.F. Sánchez & R. Tampé,...
Abstract Membrane receptors are central to cell-cell communication. Receptor clustering at the plasma membrane modulates physiological responses, and mesoscale receptor organization is critical for downstream signaling. Spatially restricted cluster formation of neuropeptide Y 2 hormone (Y R) was observed in vivo ; however, relevance this confinement not fully understood. Here, we controlled R situ by a chelator nanotool. Due multivalent interaction, dynamic exchange microscale confined...
ABSTRACT Alzheimer’s disease (AD) is characterized by a cognitive impairment associated to amyloid beta (Aβ) aggregation and deposition in the brain. Aβ generated sequential cleavage of precursor protein (APP) β-site APP cleaving enzyme 1 (BACE1) γ-secretase complex. The mechanisms that underlie exacerbated production Aβ, favoring its brain, largely unknown. In vitro studies have shown aggregates trigger enhanced yet non described mechanism. Here, we show different cell types, including...