A5081374729- Natural Antidiabetic Agents Studies
- Psidium guajava Extracts and Applications
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Phytochemistry and Biological Activities
- Phytochemicals and Antioxidant Activities
- Saffron Plant Research Studies
- Lysosomal Storage Disorders Research
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Ethnobotanical and Medicinal Plants Studies
- Chemotherapy-induced organ toxicity mitigation
- Botanical Research and Chemistry
- Bioactive natural compounds
- Phytochemicals and Medicinal Plants
- Enzyme function and inhibition
- Gold and Silver Nanoparticles Synthesis and Applications
- Protein Kinase Regulation and GTPase Signaling
- Selenium in Biological Systems
- Medicinal Plant Research
- Autoimmune and Inflammatory Disorders Research
- Viral Infectious Diseases and Gene Expression in Insects
- Papaya Research and Applications
- Nigella sativa pharmacological applications
- Advanced Biosensing Techniques and Applications
National Institutes of Health
2024
National Cancer Institute
2024
Center for Cancer Research
2024
Indian Institute of Chemical Biology
2004-2023
Jadavpur University
2021
Himalayan University
2011
Seoul National University
2010
In-Q-Tel
2010
Recently, we described that glyceraldehyde-3-phosphate dehydrogenase from Leishmania major (LmGAPDH) was present in extracellular vesicles and it inhibited host TNF-alpha expression during infection via post-transcriptional repression. The LmGAPDH binding with the AU-rich elements 3'-untranslated region of mRNA (TNF-alpha ARE) is sufficient for limiting this cytokine production, but ARE residues are still unexplored. RNA electrophoretic mobility shift assay (REMSA) catalytic activity...
Fabrication of a SERS-active substrate using Langmuir–Blodgett and self-assembly techniques for the detection diabetes from blood plasma samples.
Per-Arnt-Sim (PAS) domains are structurally conserved and present in numerous proteins throughout all branches of the phylogenetic tree. Although PAS domain-containing major players for adaptation to environmental stimuli both prokaryotic eukaryotic organisms, these types still uncharacterized trypanosomatid parasites, Trypanosome Leishmania In addition, PAS-containing phosphoglycerate kinase (PGK) protein is literature. Here, we report a PGK (LmPAS-PGK) unicellular pathogen The modeled...
Recently, we described the PAS domain‐containing phosphoglycerate kinase (PGK) from Leishmania major (LmPAS‐PGK) that shows acidic pH (5.5)‐dependent optimum catalytic activity. The domain of LmPAS‐PGK is expected to regulate PGK activity during catalysis, but mechanism regulation by at molecular level uncharacterized. In this work, have utilized full‐length, domain‐deleted, and mutant enzymes measure enzymatic in presence divalent cation various values. Catalytic measurement indicates Mg 2+...
Objective: The objective of the present study was to carry out presence antioxidants activity on root extract Dilleniaindica (Family-Dilleniaceae) which is believed have protective mechanisms in beneficial health effects.
 Methods: Considering its medicinal importance, plant chosen for extraction with various solvents such as petroleum ether, chloroform and ethanol taken into considerations determine phytochemicals analyses it. extracts roots were evaluated antioxidant by using...
The ChaC family of γ-glutamyl cyclotransferases is conserved throughout all Kingdoms and catalyzes the degradation GSH. So far, proteins in trypanosomal parasites are missing literature. Here, we report two members (LmChaC2a LmChaC2b) unicellular pathogen Leishmania. Activity measurements suggest that these catalyze GSH but no other peptides. Recombinant LmChaC2a protein shows ∼17-fold lower catalytic efficiency (kcat ∼ 0.9 s-1) than LmChaC2b 15 s-1), although they showed comparable Km...
Surface plasmon resonance (SPR) is a well-known optical technique for detection of protein absorption or dielectric concentration on metal surface. In this study, we developed an angle-scanning SPR biosensor that accurately measures the amount mineralization using MC3T3-el cells without any damage to cells. Since there have been few accurate methods quantitatively measure osteogenesis, our results are compared with traditional method Alizarin red S staining. The result showed sensor much...
Objective: To determine the antioxidant activity of Nyctanthes arbortristis (Family-Oleaceae).Methods: The hydroalcoholic extract root plant was taken into considerations to phytochemicals present in it. extracts roots were evaluated for by using different vitro model like Reducing Power Method and DPPH method.Results: In current investigation it has been found that Pet. Ether Hydroalcoholic showed potent reducing power, as concentration increased, absorbance also increased...
Objective: To estimate the anti-microbial activity of hydro-alcoholic (methanol) and petroleum ether extract Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The whole root part plant was prepared studied for constituents by using various standard methods. antimicrobial performed on two bacterial strains one fungal strain disc diffusion method.Results: present study shows analysis, arbour-tristis. Various analyses revealed presence...
Objective: To estimate the anthelmintic activity of hydro-alcoholic (methanol) and petroleum ether extract Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The whole root part plant was prepared. And studies Phytochemical constitutes by various standard method. performed a different material were tested against adult Indian earthworms pheretima posthuma as test worms, bioassay determines time paralysis death. Albendazole is used reference...
An in vitro study of the methanolic extract whole plant (except flower) Thunbergia coccinea was performed to check anti-oxidant activity by different methods (Reducing power assay, TBA and DPPH scavenging assay) before starting study, extracted Soxhlet apparatus using methanol as solvent marc evaporated kept under desiccator.From literature survey it found that contain flavonoids tannins which are non-enzymatic antioxidants responsible for free radicals reported based on this, antioxidant...