A5079633401- Cholesterol and Lipid Metabolism
- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Drug Transport and Resistance Mechanisms
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Immune Response and Inflammation
- Bariatric Surgery and Outcomes
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and disorders
- Fatty Acid Research and Health
- Diet and metabolism studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Atherosclerosis and Cardiovascular Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Gastroesophageal reflux and treatments
- Sepsis Diagnosis and Treatment
- Esophageal and GI Pathology
- Diabetes Treatment and Management
- Cardiac, Anesthesia and Surgical Outcomes
- Immune Cell Function and Interaction
- Immune cells in cancer
- Enhanced Recovery After Surgery
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
Université de Bourgogne
2015-2025
Inserm
2016-2025
Fondation de Coopération Scientifique
2015-2024
Centre de recherche Translationnelle en Médecine moléculaire
2011-2024
Université Bourgogne Franche-Comté
2015-2024
CHU Dijon Bourgogne
2011-2023
Immunology from Concept and Experiments to Translation
2023
Université de Caen Normandie
2012-2021
Institut Agro Dijon
2011-2021
Institut Bouisson Bertrand
2018-2019
Mitochondria have emerged as key actors of innate and adaptive immunity. Mitophagy has a pivotal role in cell homeostasis, but its contribution to macrophage functions host defense remains be delineated. Here, we showed that lipopolysaccharide (LPS) combination with IFN-γ inhibited PINK1-dependent mitophagy macrophages through STAT1-dependent activation the inflammatory caspases 1 11. In addition, demonstrated inhibition triggered classical mitochondrial ROS-dependent manner. murine model...
Quantitation of plasma lipopolysaccharides (LPSs) might be used to document Gram-negative bacterial infection. In the present work, LPS-derived 3-hydroxymyristate was extracted from samples with an organic solvent, separated by reversed phase HPLC, and quantitated MS/MS. This mass assay combined limulus amebocyte lysate (LAL) bioassay monitor neutralization LPS activity in biological samples. The described HPLC/MS/MS method is a reliable, practical, accurate, sensitive tool quantitate LPS....
Type 2 diabetes mellitus (T2DM) is a major global health issue and significant risk factor for atherosclerosis. Atherosclerosis in T2DM patients has been associated with inflammation, insulin resistance, hyperglycemia, dyslipidemia, oxidative stress. Identifying molecular features of atherosclerotic plaques could provide valuable insights into the pathogenesis disease.The MASCADI (Arachidonic Acid Metabolism Carotid Stenosis Plaque Diabetic Patients) study aimed to investigate increase...
BACKGROUND: During atherogenesis, macrophages turn into foam cells by engulfing lipids present within the atheroma plaques. The shift of toward proinflammatory or anti-inflammatory phenotypes, a critical step in disease progression, is still poorly understood. LXRs (liver X receptors) play pivotal role macrophage response to lipid, promoting expression key genes cholesterol efflux, mitigating intracellular accumulation. also exert balanced actions on inflammation human macrophages,...
The aim of the present study was to identify protein that accounts for cholesteryl ester transfer (CETP)-inhibitory activity is specifically associated with human plasma high density lipoproteins (HDL). To this end, HDL apolipoproteins were fractionated by preparative polyacrylamide gradient gel electrophoresis, and 30 distinct fractions molecular masses ranging from 80 down 2 kDa tested their ability inhibit CETP activity. One single apolipoprotein fraction able completely N-terminal...
Although cholesteryl ester transfer protein (CETP) inhibition is regarded as a promising strategy to reduce atherosclerosis by increasing high-density lipoprotein cholesterol, the CETP inhibitor torcetrapib given in addition atorvastatin had no effect on and even increased cardiovascular death recent Investigation of Lipid Level Management Understand its Impact Atherosclerotic Events trial. Therefore, we evaluated antiatherogenic potential adverse effects humanized APOE*3-Leiden.CETP...
Objective— Liver X receptors (LXRs) are oxysterol-activated nuclear that highly expressed in macrophages and regulate lipid homeostasis inflammation. Among putative LXR target genes, lysophosphatidylcholine acyltransferase 3 (LPCAT3) involved the Lands cycle controls fatty acid composition at sn-2 position of glycerophospholipids and, therefore, availability acids, such as arachidonic (AA), used for eicosanoid synthesis. The aim our study was to determine whether LXRs could human...
Liver X receptors (LXRs) modulate cholesterol and fatty acid homeostasis as well inflammation. This study aims to decipher the role of LXRs in regulation polyunsaturated (PUFA) synthesis macrophages context atherosclerosis.Transcriptomic analysis human monocytes was used identify putative LXR target genes among enzymes involved PUFA biosynthesis. In parallel, consequences activation or invalidation on distribution were determined. Finally, we investigated impact metabolism vivo...
Objectives: Systemic inflammatory response syndrome and sepsis frequently occur after cardiac surgery with cardiopulmonary bypass. The aim of the present study was to investigate whether preoperative cholesterol levels can predict onset postoperative complications in patients undergoing Design: Prospective observational study. Setting: Surgical ICU a French university hospital. Patients: Two hundred seventeen consecutive older than 18 years admitted for planned Interventions: Measurements...
Lipopolysaccharides (LPS) are components of Gram-negative bacteria. The cellular response from the host to LPS is mediated through stepwise interactions involving lipopolysaccharide-binding protein (LBP), CD14, and MD-2, which produces rearrangement TLR4. In addition LBP, lipid transfer/lipopolysaccharide-binding gene family includes phospholipid transfer (PLTP). Here we show that intravascular redistribution plasma lipoprotein-free fraction toward circulating lipoproteins delayed in...
Endothelial lipase (EL) is a negative regulator of high density lipoprotein (HDL) cholesterol plasma levels, and scavenger receptor BI (SR-BI) involved in remodeling HDL. The present study investigates the requirement SR-BI for effects EL-mediated phospholipid hydrolysis on HDL metabolism vivo. In vitro, selective uptake from EL-modified was 129% higher than control SR-BI-overexpressing cells (p=0.01). vivo overexpression human EL by means recombinant adenovirus decreased levels...
Plasma PLTP (phospholipid-transfer protein) is a member of the lipid transfer/LBP [LPS (lipopolysaccharide)-binding protein] family, which constitutes superfamily genes together with short and long PLUNC (palate, lung nasal epithelium clone) proteins. Although was studied initially for its involvement in metabolism HDL (high-density lipoproteins) reverse cholesterol transport (i.e. metabolic pathway through excess can be transported from peripheral tissues back to liver excretion bile), it...
OBJECTIVE Apolipoprotein (apo)C1 is a potent physiological inhibitor of cholesteryl ester transfer protein (CETP). ApoC1 operates through its ability to modify the electrostatic charge at lipoprotein surface. We aimed determine whether inhibitory apoC1 still effective in vivo patients with diabetes and vitro glycation influences CETP effect. RESEARCH DESIGN AND METHODS concentrations activity were measured 70 type 1 diabetic (T1D) patients, 113 2 diabetes, 83 control subjects. The...
Cholesteryl ester transfer protein (CETP) activity results in a proatherogenic lipoprotein profile. In cholestatic conditions, farnesoid X receptor (FXR) signaling by bile acids (BA) is activated and plasma HDL cholesterol (HDL-C) levels are low. This study tested the hypothesis that FXR-mediated induction of CETP contributes to this phenotype. Patients with cholestasis high BA had lower HDL-C higher mass compared matched controls low (each P < 0.01). feeding APOE3*Leiden transgenic mice...
Plasma phospholipid transfer protein (PLTP) is involved in intravascular lipoprotein metabolism. PLTP known to act through 2 main mechanisms: by remodeling high-density lipoproteins (HDL) and increasing apolipoprotein (apo) B-containing lipoproteins. The aim of this study was generate a new model human transgenic (HuPLTPTg) rabbit determine whether expression modulates atherosclerosis species that, unlike humans mice, displays naturally very low activity.In HuPLTPTg rabbits, the cDNA placed...
Abstract COVID-19 pneumonia has specific features and outcomes that suggests a unique immunopathogenesis. Severe forms of appear to be more frequent in obese patients, but an association with metabolic disorders is not established. Here, we focused on lipoprotein metabolism patients hospitalized for severe pneumonia, depending status. Thirty-four non-COVID-19 27 were enrolled. Most them required intensive care. Plasma lipid levels, metabolism, clinical biological (including plasma cytokines)...
Genetically engineered mice demonstrated that apolipoprotein (apo) CI is a potent, physiological inhibitor of plasma cholesteryl ester transfer protein (CETP) activity. The goal this study was to determine the molecular mechanism apoCI-mediated blockade CETP Kinetic analyses revealed inhibitory property apoCI independent amount active CETP, but it tightly dependent on high density lipoproteins (HDL) in incubation mixtures. electrostatic charge HDL, i.e. main carrier human plasma, gradually...
The H2 allele of APOC1, giving rise to increased gene expression apolipoprotein C-I (apoC-I), is in genetic disequilibrium with the APOE4 and may provide a major risk factor for Alzheimer's disease (AD). We found that apoC-I protein present astrocytes endothelial cells within hippocampal regions both human control AD brains. Interestingly, colocalized beta-amyloid (Abeta) plaques brains, vitro experiments revealed aggregation Abeta was delayed presence apoC-I. Moreover, exacerbate soluble...