A5101807940- Electrospun Nanofibers in Biomedical Applications
- Connective tissue disorders research
- 3D Printing in Biomedical Research
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- MicroRNA in disease regulation
- Retinal Diseases and Treatments
- Cell Adhesion Molecules Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiac and Coronary Surgery Techniques
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Gastrointestinal Tumor Research and Treatment
- Drug-Induced Adverse Reactions
- Epigenetics and DNA Methylation
- Angiogenesis and VEGF in Cancer
- Sirtuins and Resveratrol in Medicine
- Pluripotent Stem Cells Research
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Connexins and lens biology
- Cancer Genomics and Diagnostics
- Skin Protection and Aging
- Reproductive System and Pregnancy
Xi'an Medical University
2020-2023
Trinity College Dublin
2019-2021
University of Limerick
2019
University of Tübingen
2015-2017
Fraunhofer Institute for Interfacial Engineering and Biotechnology
2013-2017
Liuzhou General Hospital
2007
Xi'an Jiaotong University
2006
Huadong Hospital
2001
Currently available heart valve replacements are limited in long-term performance or fail due to leaflet thickening, lack of growth remodeling potential. In order address these issues, it is necessary mimic multiple factors the native valvular extracellular matrix (ECM) such as architecture, mechanical behavior and biochemical signals. Here, we successfully generated an electrospun PEGdma–PLA scaffold adapted structure properties leaflets. Valvular interstitial cells (VICs) endothelial...
Abstract Bone formation or regeneration requires the recruitment, proliferation, and osteogenic differentiation of stem/stromal progenitor cells. A potent stimulus driving this process is mechanical loading. Osteocytes are mechanosensitive cells that play fundamental roles in coordinating loading-induced bone via secretion paracrine factors. However, exact mechanisms by which osteocytes relay signals to these poorly understood. Therefore, study aimed demonstrate potency mechanically...
Elastic fibers are essential for the proper function of organs including cardiovascular tissues such as heart valves and blood vessels. Although (tropo)elastin production in a tissue-engineered construct has previously been described, assembly to functional elastic vitro using human cells highly challenging. In present study, we seeded primary isolated vascular smooth muscle (VSMCs) onto 3D electrospun scaffolds exposed them defined laminar shear stress customized bioreactor system....
Cardiovascular disease remains a leading cause of mortality and morbidity worldwide. Embryonic stem cell-derived cardiomyocytes (ESC-CMs) may offer significant advances in creating vitro cardiac tissues for modeling, drug testing, elucidating developmental processes; however, the induction ESCs to more adult-like CM phenotype challenging. In this study, we developed bioreactor system employ pulsatile flow (1.48 mL/min), cyclic strain (5%), extended culture time improve maturation murine...
One major obstacle to the application of stem cell-derived cardiomyocytes (CMs) for disease modeling and clinical therapies is inability identify developmental stage these cells without need genetic manipulation or utilization exogenous markers. In this study, we demonstrate that Raman microspectroscopy can non-invasively embryonic cell (ESC)-derived chamber-specific CMs monitor maturation. Using marker-free approach, peaks were identified atrial ventricular CMs, ESCs successfully...
Synthetic polymeric materials have demonstrated great promise for bone tissue engineering based on their compatibility with a wide array of scaffold-manufacturing techniques, but are limited in terms the bioactivity when compared to naturally occurring materials. To enhance regenerative properties these materials, they commonly functionalised bioactive factors guide growth within developing tissue. Extracellular matrix vesicles (EVs) play an important role facilitating endochondral...
Bioreactors are essential cell and tissue culture tools that allow the introduction of biophysical signals into in vitro cultures. One major limitation is need to interrupt experiments sacrifice samples at certain time points for analyses. To address this issue, we designed a bioreactor combines high-resolution contact-free imaging continuous flow closed system compatible with various types microscopes. The high throughput fluid was combined two-photon fluorescence lifetime microscopy...
Human distal upstream element (Fuse) binding protein 1 (FUBP1) is a transcriptional regulator of c-Myc and represents an important prognostic marker in many cancers. Therefore, the present study aimed to investigate whether FUBP1 could combine with participate progression colon cancer. Detection expression was done through reverse transcription-quantitative PCR (RT-qPCR), combination detected by immunoprecipitation assay. Cell counting kit (CCK)-8, colony formation, transwell wound healing...
Tracking the distribution and differentiation of stem cells by high-resolution imaging techniques would have significant clinical research implications. In this study, a model cell-penetrating peptide was used to carry gadolinium particles for magnetic resonance (MRI) mesenchymal (MSCs). MSCs were isolated from rat bone marrow identified osteogenic in vitro. The labeled with fluorescein-5-isothiocyanate (FITC) synthesized solid-phase synthesis method. Fluorescein analysis confirmed that new...
Abstract Osteocytes are mechanosensitive cells that believed to play a fundamental role in coordinating bone mechanoadaptation via the secretion of paracrine factors. However, exact mechanisms by which osteocytes relay mechanical signals effector is poorly understood. In this study, we demonstrated subjected physiologic fluid shear secrete distinct collection factors significantly enhance human MSC recruitment and osteogenesis. Utilising proteomics generated an extensive map proteins within...
Fibroblast growth factor 21 (FGF21) is secreted by hepatocytes as a peptide hormone to regulate glucose and lipid metabolism. FGF21 promotes hepatic ketogenesis increases ketone body utilization in starvation. Histones are the target molecules of nutrients regulating metabolic homeostasis. However, effect bodies on expression involvement histones it not clear yet. The present study observed effects β-hydroxybutyrate (β-OHB), main physiological body, human hepatoma HepG2 cells vitro mice...
Background: Elastic fibers are one of the main components extracellular matrix (ECM), providing dynamic tissues such as lungs, skin, blood vessels, heart valve leaflets and ligaments with flexibility elasticity. However, complex process elastic fiber assembly is poorly understood und generation on a human base missing link in tissue engineering. Methods: A constant shear stress providing, fluid flow bioreactor system was designed evaluated. For vitro studies, vascular smooth muscle cells...
Background: Retinal disorders cause substantial visual burden globally. Accurate estimates of the prevalence and causes loss related to retinal diseases are pivotal inform optimal eye health care planning reallocation medical resources. However, comparative rates regarding contribution disabilities caused by common have not been assessed on a national scale. The purpose this study is describe vision impairment blindness in patients with diseases.Methods: A cross-sectional hospital-based...