A5083748340- Microfluidic and Bio-sensing Technologies
- Cellular Mechanics and Interactions
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Block Copolymer Self-Assembly
- Adhesion, Friction, and Surface Interactions
- 3D Printing in Biomedical Research
- Blood properties and coagulation
- Nuclear Structure and Function
- Surface Modification and Superhydrophobicity
- Sperm and Testicular Function
- Digital Holography and Microscopy
- Reproductive Biology and Fertility
- Skin and Cellular Biology Research
- Cell Image Analysis Techniques
- Electrohydrodynamics and Fluid Dynamics
- RNA Research and Splicing
- Electrical and Bioimpedance Tomography
- Reproductive biology and impacts on aquatic species
- Nanopore and Nanochannel Transport Studies
University of Naples Federico II
2019-2025
Intrinsic biophysical and morphological features are essential for the label-free identification of different cell types. Indeed, apart from object size, density could represent a key parameter single-cell analysis. However, measurement such is challenging. Therefore, we present straightforward versatile microfluidic chip. The densimeter-on-chip (DoC) measures mass densities thanks to hydrodynamically induced sedimentation process inside microchannel. In detail, in-flow buoyant components...
Cell deformability is a well-established marker of cell states for diagnostic purposes. However, the measurement wide range different levels still challenging, especially in cancer, where large heterogeneity rheological/mechanical properties present. Therefore, simple, versatile and cost-effective recognition method variable cells needed. Here, we introduce new set in-flow motion parameters capable identifying among deformability, properly modified by administration drugs cytoskeleton...
The cell nucleus plays a critical role in mechanosensing and mechanotransduction processes, by adaptive changes of its envelope composition to external biophysical stimuli such as substrate rigidity tensile forces. Current measurement approaches lack precise control stress application on nuclei, thus significantly impairing complete mechanobiological study cells. Here, we present contactless microfluidic approach capable exert wide range viscoelastic compression forces (10-103 µN)-as an...
Tuneable viscoelastic forces and a properly engineered microfluidic design induce in-flow deformation-dependent dynamics for cell mechanical measurements.
Mammalian sperm motility is getting more relevant due to rising infertility rates worldwide, generating the need improve conventional analysis and diagnostic approaches. Nowadays, computer assisted (CASA) technologies represent a popular alternative manual examination which generally performed by observing in very confined geometries. However, under physiological conditions, describe three-dimensional patterns are not well reconstructed limited depth of standard acquisition chambers....
In recent years, nuclear mechanobiology gained a lot of attention for the study cell responses to external cues like adhesive forces, applied compression, and/or shear-stresses. details, Lamin-A protein-as major constituent nucleus structure-plays crucial role in overall mechanobiological response. However, modeling and analysis protein organization upon rapid compression conditions microfluidics are still difficult be performed. Here, we introduce possibility control an microfluidic on...
Abstract Background Among mechanoporation techniques for intracellular delivery, microfluidic approaches succeed in high delivery efficiency and throughput. However, especially the entry of large cargoes (e.g. DNA origami, mRNAs, organic/inorganic nanoparticles) is currently impaired since it requires cell membrane pores with need to apply multi-step processes forces, dramatically reducing viability. Results Here, HiViPore presents as a viscoelastic contactless compression one-step produce...
We investigated subclasses of living peripheral blood cells in a microfluidic-based system, with the aim to characterize their morphometric and optical properties, track position flow label-free modality. employed two coherent imaging techniques: scattering approach precisely aligned single cells, digital holography achieve cell reconstructions flow. Cells were first 3D-aligned round shaped capillary subsequently measured following square channel. Results obtained at fixed measurement...
The microfluidic based characterization (static light scattering) of morphometric and optical cell properties over time related to an in-flow applied compressive force can reveal useful insides in cell-mechanical processes.