A5060881837- Biochemical and Molecular Research
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- Click Chemistry and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Protein Structure and Dynamics
- HIV/AIDS drug development and treatment
- Plant biochemistry and biosynthesis
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Cassava research and cyanide
- Cell Image Analysis Techniques
- Lipid metabolism and biosynthesis
- Phosphorus compounds and reactions
- Heat shock proteins research
- Synthetic Organic Chemistry Methods
- Bacterial Identification and Susceptibility Testing
- Pancreatic and Hepatic Oncology Research
- Advanced Biosensing Techniques and Applications
- Polyamine Metabolism and Applications
- RNA and protein synthesis mechanisms
Purdue University West Lafayette
2014-2025
Center for Cancer Research
2017-2018
State Street (United States)
2010
University of Illinois Urbana-Champaign
2005-2007
University of Notre Dame
2007
McGill University
2005
Biotechnology Research Institute
2005
National Academies of Sciences, Engineering, and Medicine
2005
Indiana University – Purdue University Indianapolis
1985-2005
Walther Cancer Foundation
2003-2005
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhosphorylation of isoprenoid alcoholsV. Jo Davisson, Andrew B. Woodside, Timothy R. Neal, Kay E. Stremler, Manfred Muehlbacher, and C. Dale PoulterCite this: J. Org. Chem. 1986, 51, 25, 4768–4779Publication Date (Print):December 1, 1986Publication History Published online1 May 2002Published inissue 1 December 1986https://pubs.acs.org/doi/10.1021/jo00375a005https://doi.org/10.1021/jo00375a005research-articleACS PublicationsRequest reuse...
Intact, multiply protonated proteins of particular mass and charge were selected from ionized protein mixtures gently landed at different positions on a surface to form microarray. An array cytochrome c, lysozyme, insulin, apomyoglobin was generated, the deposited showed electrospray ionization spectra that matched those authentic compounds. Deposited lysozyme trypsin retained their biological activity. Multiply charged ions kinase A catalytic subunit hexokinase also soft-landed into...
The compatibility of nonenhanced Raman spectroscopy with chromatographic and mass spectroscopic proteomic sensing is demonstrated for the first time. High-quality normal spectra are derived from protein solutions concentrations down to 1 microM fmol nondestructively probed within excitation laser beam. These results obtained using a drop coating deposition (DCDR) method in which solution interest microdeposited (or microprinted) on compatible substrate, followed by solvent evaporation...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of nucleotide 5'-diphosphates from 5'-O-tosyl nucleosidesV. Jo Davisson, Darrell R. Davis, Vyas M. Dixit, and C. Dale PoulterCite this: J. Org. Chem. 1987, 52, 9, 1794–1801Publication Date (Print):May 1, 1987Publication History Published online1 May 2002Published inissue 1 1987https://pubs.acs.org/doi/10.1021/jo00385a026https://doi.org/10.1021/jo00385a026research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract Background Breast cancer is a collection of diseases in which molecular phenotypes can act as both indicators and mediators therapeutic strategy. Therefore, candidate therapeutics must be assessed the context multiple cell lines with known phenotypes. Docosahexaenoic acid (DHA) curcumin (CCM) are dietary compounds to antagonize breast proliferation. We report that these combination exert variable antiproliferative effect across lines, synergistic SK-BR-3 cells triggers signaling...
Expression and function of the human papillomavirus (HPV) early protein 6 (E6) is necessary for viral replication oncogenesis in cervical cancers. HPV E6 targets tumor suppressor p53 degradation. To achieve this, "high-risk" proteins bind to modify target specificity ubiquitin ligase E6AP (E6 associated protein). This E6-dependent loss enables virus bypass host cell defenses facilitates virally induced activation cycle progression during replication. Disruption interaction between...
Our present work demonstrates the successful design and synthesis of a new class compounds based upon multitargeted directed ligand approach to discover agents for use in Alzheimer's disease (AD). All were tested their vitro inhibitory potential against human acetylcholinesterase (hAChE), butylcholinesterase (hBChE), β-secretase-1 (hBACE-1), amyloid β (Aβ) aggregation. Compounds 5d 5f have shown hAChE hBACE-1 inhibition comparable donepezil, while hBChE was rivastigmine. also demonstrated...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImidazole glycerol phosphate synthase: The glutamine amidotransferase in histidine biosynthesisThomas J. Klem and V. Jo DavissonCite this: Biochemistry 1993, 32, 19, 5177–5186Publication Date (Print):May 18, 1993Publication History Published online1 May 2002Published inissue 18 1993https://pubs.acs.org/doi/10.1021/bi00070a029https://doi.org/10.1021/bi00070a029research-articleACS PublicationsRequest reuse permissionsArticle...
A protein sensor based on nanostructured adaptive silver films (ASFs) is developed for soft adsorption and detection with surface-enhanced Raman scattering (SERS). Under deposition, the ASFs modify their local nanostructure so that conformational state of proteins preserved SERS optimized. The used to examine differences in spectra two insulin isomers, human its analogue lispro. These insulins differ only interchange neighboring amino acids; specifically, propyl−lysyl sequence at C-terminus...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTN5-Carboxyaminoimidazole Ribonucleotide: Evidence for a New Intermediate and Two Enzymic Activities in the de Novo Purine Biosynthetic Pathway of Escherichia coliErnest J. Mueller, Erik Meyer, Johannes Rudolph, V. Jo Davisson, JoAnne StubbeCite this: Biochemistry 1994, 33, 8, 2269–2278Publication Date (Print):March 1, 1994Publication History Published online1 May 2002Published inissue 1 March...
The crystal structure of human thymidylate synthase, a target for anti-cancer drugs, is determined to 3.0 A resolution and refined crystallographic residual 17.8%. implicates the enzyme in mechanism facilitating docking substrates into active site. This involves twist approximately 180 degrees site loop, pivoted around neighboring residues 184 204, ordering external, eukaryote specific loops along with well-characterized closure upon substrate binding. highly conserved, but...
We have combined equilibrium and steered molecular dynamics (SMD) simulations with principal component correlation analyses to probe the mechanism of allosteric regulation in imidazole glycerol phosphate (IGP) synthase. An evolutionary analysis IGP synthase revealed a conserved network interactions leading from effector binding site glutaminase active site, forming communication pathways between remote sites. SMD undocking ribonucleotide N1-[(5'-phosphoribulosyl)-formino]-5'-aminoimidazole...
Acivicin [(αS,5S)-α-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid] was investigated as an inhibitor of the triad glutamine amidotransferases, IGP synthase and GMP synthetase. Nucleophilic substitution chlorine atom in acivicin results formation imine−thioether adduct at active site cysteine. Cys 77 identified modification heterodimeric IGPS from Escherichia coli (HisHF) by tryptic digest FABMS. Distinctions glutaminase domains E. coli, bifunctional protein Saccharomyces cerevisiae...
A cDNA clone encoding thymidylate synthase (TS) has been isolated from a human T-cell library and modified in the 5'-untranslated region to incorporate several unique cloning sites. The gene cloned as cassette into Escherichia coli expression vectors which did not provide detectable amounts of enzyme. successful approach used constitutive E. vector developed for enzyme Lactobacillus casei. 115-base pair L. casei TS contains ribosomal binding site other regulatory sequences fused coding...
Ester derivatives of brefeldin A (BFA) were synthesized to determine which its two hydroxyl groups could be modified while still maintaining biological activity. The compounds tested for antiproliferative activity in the National Cancer Institute's 60 cancer cell line screen. Monoderivatization at C4 and C7 alcohols was tolerated, yielding biologically active compounds, whereas analogues derivatized both positions least series. Molecular modeling revealed that well tolerated interface...
Screening of a Gossypium arboreum L. cv. Nanking cDNA library resulted in the identification and cloning second (+)-δ-cadinene synthase. A probe for screens was prepared by PCR using primers based on conserved sequences farnesyl diphosphate cyclases genomic DNA as template. This clone encodes protein that is 80% identical to recently described synthases CAD1-C1 C14 from G. maintains significant degree homology other known mono-, sesqui-, diterpene synthases. As case synthase cultured cotton...
Imidazole glycerol phosphate synthase catalyzes formation of the imidazole ring in histidine biosynthesis. The enzyme is also a glutamine amidotransferase, which produces ammonia glutaminase active site and channels it through 30-Å internal tunnel to cyclase site. Glutaminase activity impaired resting enzyme, stimulated by substrate binding signaling mechanism was investigated crystal structure ternary complex inactivated analogue unstable cryo-trapped orientation...