Many stimuli, such as hormones and abiotic stress factors, elicit changes in intracellular calcium levels that serve to convey information activate appropriate responses. The Ca2+ signals are perceived by different receptors, calmodulin (CaM) is one of the best-characterized sensors eukaryotes. Calmodulin-like (CML) proteins also exist plants; they share sequence similarity with ubiquitous highly conserved CaM, but their roles at physiological molecular largely unknown. We present data on...

Plants overcome water deficit conditions by combining molecular, biochemical and morphological changes. At the molecular level, many stress-responsive genes have been isolated, but knowledge of their physiological functions remains fragmentary. Here, we report data for RD20, a stress-inducible Arabidopsis gene that belongs to caleosin family. As other caleosins, showed RD20 localized oil bodies. Although caleosins are thought play role in degradation lipids during seed germination, induction...

A clone for a novel Arabidopsisthaliana calmodulin (CaM)-binding protein of 25 kDa (AtCaMBP25) has been isolated by using radiolabelled CaM probe to screen cDNA expression library derived from A. thaliana cell suspension cultures challenged with osmotic stress. The deduced amino acid sequence AtCaMBP25 contains putative nuclear localization sequences and shares significant degree similarity hypothetical plant proteins only. Fusion the coding reporter genes targets hybrid nucleus. Bacterially...

Summary Many stimuli such as hormones and elicitors induce changes in intracellular calcium levels to integrate information activate appropriate responses. The Ca 2+ signals are perceived by various sensors, calmodulin (CaM) is one of the best characterized eukaryotes. Calmodulin‐like (CML) proteins extend toolkit plants; they share sequence similarity with ubiquitous highly conserved CaM but their roles at physiological molecular largely unknown. Knowledge contribution decoding plant...

It is well known that calcium, ethylene and abscisic acid (ABA) can regulate fruit ripening, however, their interaction in the regulation of ripening has not yet been fully clarified. The present study found expression papaya calcium sensor CpCML15 was strongly linked to ripening. could bind Ca

The calmodulin (CaM) family is a major class of calcium sensor proteins which collectively play crucial role in cellular signaling cascades through the regulation numerous target proteins. Although CaM one most conserved all eukaryotes, several features and its downstream effector are unique to plants. continuously growing repertoire CaM-binding includes plant-specific Plants also possess particular set isoforms CaM-like (CMLs) whose functions have just begun be elucidated. This review...

Summary The heptapeptide Tyr‐Gly‐ Arg‐Gly‐Asp‐ Ser‐Pro containing the sequence Arg‐Gly‐Asp (RGD – essential structure recognised by animal cells in substrate adhesion molecules) was tested on epidermal of onion and cultured Arabidopsis upon plasmolysis. Dramatic changes were observed both types following treatment: cells, Hechtian strands linking cell wall to membrane lost, while changed from concave convex A control Tyr‐Gly‐Asp‐Gly‐Arg‐Ser‐Pro had no effect shape plasmolysed cells....

Calcium is a universal second messenger involved in various cellular processes including plant development and stress responses. Its conversion into biological responses requires the presence of calcium sensor relays such as calmodulin (CaM) calmodulin-like (CML) proteins. While role CaM well described, functions CML proteins remain largely uncharacterized. Here, we show that Arabidopsis CML8 expression strongly transiently induced by Pseudomonas syringae, reverse genetic approaches...

In their natural environment, plants have to continuously face constraints such as biotic and abiotic stresses. To achieve life cycle, perceive interpret the nature, but also strength of environmental stimuli activate appropriate physiological responses. Nowadays, it is well established that signaling pathways are crucial steps in implementation rapid efficient plant responses genetic reprogramming. It reported raises calcium (Ca2+) levels within cells participate these early essential...

Changes in the level of cytosine methylation were assessed by HPLC mechanically stressed Bryonia dioica . Rubbing young internodes. which results growth inhibition and ethylene production, induced a rapid transient decrease DNA. The was about 25% internodes dropped to nearly 0% less than 1 h before increasing normal within 3 h. A decreasing gradient DNA occurred naturally along plant, from apex base. pool S‐adenosylmethionine also measured order establish possible relationship between...

Calcium signalling mediated by Calmodulin (CaM) and calmodulin-like (CML) proteins is critical to plant immunity. CaM CML regulate a wide range of target cellular responses. While many CaM-binding have been identified, few characterized for their specific role in Here, we report new data on the biological function CML-interacting partner, PRR2 (PSEUDO-RESPONSE REGULATOR 2), transcription factor. Until now, physiological relevance remained largely unknown. Using reverse genetic strategy A....

The membrane receptor protein from pea, peabp80, has been shown to function by in vitro binding studies, and vivo yeast mutant, as a vacuolar sorting (VSR). Families of proteins with homology peabp80 have identified many plants including Arabidopsis. family receptors, atbp80a–f (Arabidopsis thaliana 80 kDa) is highly homologous may also receptors. Interactions determinants only for atbp80b. In this paper, atbp80b was over‐ under‐expressed Transgenic that over‐expressed showed no visible...

Abstract Highly purified tonoplast and plasma membrane vesicles were isolated from microsomes of Arabidopsis thaliana by preparative free‐flow electrophoresis. The most electronegative fractions identified as using nitrate‐inhibited Mg 2+ ‐ATPase enzyme marker. least glucan‐synthase II, UDPG: sterolglucosyl‐transferase, vanadate‐inhibited markers. Other markers, latent inosine‐5′‐diphosphatase (Golgi), NADPH‐cytochrome‐ c reductase (endoplasmic reticulum) cytochrome‐ oxidase (mitochondria)...

The RD20 gene encodes a member of the caleosin family, which is primarily known to function in mobilization seed storage lipids during germination. In contrast other caleosins, expression early-induced by water deficit conditions and we recently provided genetic evidence for its positive role drought tolerance Arabidopsis. also responsive pathogen infection constitutively expressed diverse tissues organs development suggesting additional roles this caleosin. This addendum describes further...