A5016687563- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Reproductive Biology
- French Urban and Social Studies
- Regulation of Appetite and Obesity
- Plant-Microbe Interactions and Immunity
- Agriculture and Rural Development Research
- Nematode management and characterization studies
- Biochemical Analysis and Sensing Techniques
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Enzyme Production and Characterization
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Adipose Tissue and Metabolism
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- Polysaccharides and Plant Cell Walls
- Genomics and Phylogenetic Studies
- Agronomic Practices and Intercropping Systems
- Probiotics and Fermented Foods
- Immune responses and vaccinations
- Galectins and Cancer Biology
Géographie de l'environnement
2020-2024
Laboratoire des Interactions Plantes Micro-Organismes
2007-2020
Centre National de la Recherche Scientifique
1998-2019
Interaction Hôtes Agents Pathogènes
2019
Laboratoires Standa (France)
2010-2016
Institut National de la Recherche Agronomique
2000-2012
Université de Bourgogne
2006
Interactions Arbres-Microorganismes
2003
British Society for Rheumatology
1999-2002
Unité de Recherche Technologie et Analyses Laitières
1999-2001
Remorin proteins have been hypothesized to play important roles during cellular signal transduction processes. Induction of some members this multigene family has reported biotic interactions. However, no host-bacteria interactions assigned remorin until now. We used root nodule symbiosis between Medicago truncatula and Sinorhizobium meliloti study the a that is specifically induced nodulation. Here we show oligomeric protein attaches host plasma membrane surrounding bacteria controls...
Nearly 7000 Arabidopsis thaliana ‐expressed sequence tags (ESTs) from 10 cDNA libraries have been sequenced, of which almost 5000 non‐redundant submitted to the EMBL data bank. The quality used is analysed. Similarity searches in international protein banks allowed detection significant similarities a wide range proteins many organisms. Alignment with ESTs rice systematic sequencing project has amino acid motifs are conserved between two organisms, thus identifying genes encoding highly...
Abstract LYK3 is a lysin motif receptor-like kinase of Medicago truncatula, which essential for the establishment nitrogen-fixing, root nodule symbiosis with Sinorhizobium meliloti. putative receptor S. meliloti Nod factor signals, but little known how it regulated and transduces these symbiotic signals. In screen LYK3-interacting proteins, we identified M. truncatula Plant U-box protein 1 (PUB1) as an interactor domain. planta, both proteins are localized interact in plasma membrane. PUB1...
Abstract AtTCP20 is a transcription factor belonging to the Arabidopsis (Arabidopsis thaliana) TCP-P subfamily, characterized by its capacity bind site II motifs (TGGGCY). Our aim was understand role of in plant development. The expression pattern translational fusion PromTCP20:CDS20∷GUS∷GFP suggested function for several organs and stages challenged planta inducing proteins fused with either transcriptional activator domain (VP16) or repressor (EAR). Expression both modified led severe...
Summary We have focused our interest on two cis ‐regulatory elements, named site II motif and telo box, identified within the promoter of plant proliferating cellular nuclear antigen ( PCNA ) putatively involved in meristematic expression gene. A conserved topological association between motifs boxes is observed numerous genes expressed cycling cells, including several cell cycle‐related 153 Arabidopsis encoding ribosomal proteins. Meristematic a GUS reporter gene was plants under control...
Summary Myc‐ LCO s are newly identified symbiotic signals produced by arbuscular mycorrhizal ( AM ) fungi. Like rhizobial Nod factors, they lipo‐chitooligosaccharides that activate the common signalling pathway CSSP in plants. To increase our limited understanding of roles we aimed to analyse ‐induced transcriptional changes and their genetic control. Whole genome RNA sequencing ‐seq) was performed on roots Medicago truncatula wild‐type plants, dmi3 nsp1 mutants affected nodulation...
PUB1, an E3 ubiquitin ligase, which interacts with and is phosphorylated by the LYK3 symbiotic receptor kinase, negatively regulates rhizobial infection nodulation during nitrogen-fixing root nodule symbiosis in Medicago truncatula. In this study, we show that PUB1 also DOES NOT MAKE INFECTIONS 2, key kinase of common signaling pathway, required for both arbuscular mycorrhizal (AM) endosymbioses. We here expression activated successive stages colonization Rhizophagus irregularis compatible...
Referee: Dr. Philip Becraft, Zoology and Genetics/Agronomy Depts., 2116 Molecular Building, lowa State University, Ames, IA 50011 Forty-two lectin receptor kinase (lecRK)-related sequences nine related soluble legume were identified in the Arabidopsis thaliana genome. The genes are scattered as a single or gathered copies at different loci throughout five chromosomes, four predicted lecRK probably correspond to pseudogenes. Both structural alignments molecular modeling revealed striking...
Abstract To study the role of LecRK (lectin-like receptor kinase) genes in legumerhizobia symbiosis, we have characterized four Medicago truncatula Gaernt. that are most highly expressed roots. Three these genes, MtLecRK7;1, MtLecRK7;2, and MtLecRK7;3, encode proteins closely related to Class A LecRKs Arabidopsis, whereas protein encoded by fourth gene, MtLecRK1;1, is similar a B Arabidopsis LecRK. All show strongly enhanced root expression, detailed studies on MtLecRK1;1 MtLecRK7;2 revealed...
The lysin motif receptor-like kinase, NFP (Nod factor perception), is a key protein in the legume Medicago truncatula for perception of lipochitooligosaccharidic Nod factors, which are secreted bacterial signals essential establishing nitrogen-fixing legume-rhizobia symbiosis. Predicted structural and genetic analyses strongly suggest that at least part receptor, but few data available about this protein. Characterization variant encoded by mutant allele nfp-2 revealed sensitivity to...
SUMMARY A number of studies have found increased levels antibodies to human endogenous retroviruses (HERVs) in autoimmune rheumatic diseases. It is not clear whether this immune response driven by the HERV itself or cross-reactions with an exogenous virus autoantigen. To address question, we examined antibody Env protein two closely related members HERV-K family, HERV-K10 and IDDMK1,222. By immunoblotting recombinant proteins, were 32–47% 84 sera from patients disease, 29% 35 normal...
ABSTRACT Rotavirus (RV) is the main etiological agent of severe gastroenteritis in infants, and vaccination seems most effective way to control disease. Recombinant rotavirus-like particles composed viral protein 6 (VP6) VP2 (2/6-VLPs) have been reported induce protective immunity mice when administered by intranasal (i.n.) route. In this study, we show that administration 2/6-VLPs intrarectal (i.r.) route together with either cholera toxin (CT) or a CpG-containing oligodeoxynucleotide as...
Objective. To test the hypothesis that glucose‐6‐phosphate isomerase (GPI) is a novel autoantigen in RA. Methods. Eighty‐eight serum samples from 23 patients with rheumatoid arthritis (RA), 25 Sjögren's syndrome, 20 systemic lupus erythematosus and healthy controls were tested by enzyme‐linked immunosorbent assay (ELISA) using commercially available, partially purified rabbit GPI as antigen. Beside each duplicate well containing antigen (10 µg/ml), uncoated blocked wells (phosphate‐buffered...