A5031527673- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Seaweed-derived Bioactive Compounds
- Microbial Natural Products and Biosynthesis
- Cellular Mechanics and Interactions
- Cancer Treatment and Pharmacology
- Cardiovascular and exercise physiology
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Flavonoids in Medical Research
- SARS-CoV-2 and COVID-19 Research
- Algal biology and biofuel production
- Antenna Design and Analysis
- Toxin Mechanisms and Immunotoxins
- Medicinal Plants and Bioactive Compounds
- Gas Sensing Nanomaterials and Sensors
- Electrochemical Analysis and Applications
- ZnO doping and properties
- Biocrusts and Microbial Ecology
- Phytochemical compounds biological activities
- Cardiovascular Effects of Exercise
- Sports Performance and Training
- Heart Rate Variability and Autonomic Control
- Osteoarthritis Treatment and Mechanisms
- Plant tissue culture and regeneration
National Yang Ming Chiao Tung University
2016-2025
Keelung Chang Gung Memorial Hospital
2025
Academia Sinica
2012-2024
Ministry of Health and Welfare
2013-2018
National Research Institute of Chinese Medicine
2018
Fujian University of Technology
2016
Far Eastern Memorial Hospital
2006-2014
National Tsing Hua University
1994-2013
China Medical University Hospital
2013
China Medical University
2013
Fucoidan, a polysaccharide extracted from brown seaweeds, reduces tumor cell proliferation. Fucoidan inhibits the growth of breast cancer cells such as 4T1 and MDA-MB-231 decreases their colony formation. Moreover, fucoidan metastatic lung nodules in xenograft female Balb/c mice. The molecular network transforming factor β (TGFβ) receptors (TGFRs) plays an important role regulation epithelial to mesenchymal transition (EMT) cells. Using cells, we found that effectively reverses TGFR-induced...
Abstract Fucoidan, a sulfated polysaccharide extracted from brown algae, exhibits anti-cancer activity. However, the effects and mechanism of fucoidan-induced apoptosis via endoplasmic reticulum (ER) stress is unclear. In this study, we demonstrated that fucoidan prevents tumorigenesis reduces tumor size in LLC1-xenograft male C57BL/6 mice. Fucoidan induces an ER response by activating PERK-ATF4-CHOP pathway, resulting apoptotic cell death vitro vivo . Furthermore, ATF4 knockdown abolishes...
// Hsien-Yeh Hsu 1,2,3,* , Tung-Yi Lin 1,3,* Yu-Chung Wu 4,5 Shu-Ming Tsao 1 Pai-An Hwang 6 Yu-Wei Shih and Jason 7 Department of Biotechnology Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan 2 The Genomics Research Center, Academia Sinica, 3 Program Molecular University 4 Division Thoracic Surgery, Taipei Veterans General Hospital, 5 School Seafood Technology Division, Fisheries Institute, Council Agriculture, Keelung, Fordham New York, NY, USA * These authors...
Carbohydrate-based vaccines have shown therapeutic efficacy for infectious disease and cancer. The mushroom Ganoderma lucidum (Reishi) containing complex polysaccharides has been used as antitumor supplement, but the mechanism of immune response rarely studied. Here, we show that mice immunized with a l -fucose (Fuc)-enriched Reishi polysaccharide fraction (designated FMS) induce antibodies against murine Lewis lung carcinoma cells, increased antibody-mediated cytotoxicity reduced production...
Ling Zhi-8 (LZ-8), an immunomodulatory protein, is derived from and has been cloned the medicinal mushroom Ganoderma lucidum (Reishi or Zhi); this protein exhibits immunomodulating antitumor properties. We investigated effects of recombinant LZ-8 (rLZ-8) on proliferation A549 human lung cancer cells. Here, we showed that rLZ-8 inhibits cell growth correlated with increased G 1 arrest. The treatment cells activated p53 p21 expression, both arrest antigrowth effect were found to be dependent....
Activating mutation of epidermal growth factor receptor (EGFR) is correlated with malignant lung tumor. In our study, we demonstrated that recombinant LZ-8 (rLZ-8), a medicinal mushroom Ganoderma lucidum protein, induced cell cycle arrest and apoptosis by downregulating the expression wild-type mutated EGFR inhibiting downstream effectors, AKT ERK1/2 in cancer cells. We showed rLZ-8 effectively inhibited progression suppressed tumor lesions mouse model. Functional studies revealed reduced...
COVID-19 is a global epidemic. Developing adjuvant therapies which could prevent the virus from binding to cells may impair viral infection. This study produces traditional Chinese medicine formula, Jing Guan Fang (JGF), based on ancient medical texts, and examines efficacy mechanism by JGF prevents infections. reduces like symptoms. Functional studies show that inhibits formation of syncytium plaque. not toxic
ATPase-family, AAA domain containing 3A (ATAD3A) is located on human chromosome 1p36.33, and high endogenous expression may associate with radio- chemosensitivity. This study was conducted to investigate the significance of ATAD3A in glioblastoma multiforme (GBM).Clinical assessed by immunohistochemistry 67 GBM specimens, prognostic value 32 patients statistically. To vitro phenotypic effects ATAD3A, cell viability measured using a clonogenic survival assay under either knockdown or ectopic...
We investigated the effects of 2 weeks detraining on cardiopulmonary function and muscular fitness in 15 endurance-trained male athletes (age: 19-26 years; height: 176.1 ± 7.5 cm; body mass: 68.3 7.6 kg). VO2max, exercise time to exhaustion (ET), maximal stroke volume (SVmax), heart rate (HRmax), isokinetic muscle strength, endurance were measured before after detraining. determined that short-term resulted a significant decrease (p < 0.05) ET, SVmax knee extensor strength but not flexor or...
Molnupiravir is an orally bioavailable prodrug of the nucleoside analogue β-D-N4-hydroxycytidine (NHC) and used to treat coronavirus disease 2019 (COVID-19). However, pharmacokinetics transplacental transfer molnupiravir in pregnant women are still not well understood. In present study, we investigated hypothesis that NHC cross blood-placenta barrier into fetus.A multisite microdialysis coupled with a validated ultrahigh-performance liquid chromatography-tandem mass spectrometry...
Lung cancer has the highest global mortality rate of any cancer. Although targeted therapeutic drugs are commercially available, common drug resistance and insensitivity to cisplatin-based chemotherapy, a clinical treatment for lung cancer, have prompted active research on alternative therapies methods mitigating cisplatin-related complications. In this study, we investigated effect WSG, glucose-rich, water soluble polysaccharide derived from Ganoderma lucidum, Murine Lewis carcinoma (LLC1)...