A5031457825- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Veterinary Pharmacology and Anesthesia
- Hemiptera Insect Studies
- Anesthesia and Sedative Agents
- Phytoplasmas and Hemiptera pathogens
- Environmental DNA in Biodiversity Studies
- Insect symbiosis and bacterial influences
- Research on scale insects
- Insect and Arachnid Ecology and Behavior
- Neurobiology and Insect Physiology Research
- Anesthesia and Pain Management
- Species Distribution and Climate Change
- Entomopathogenic Microorganisms in Pest Control
- Insect Pest Control Strategies
- Anesthesia and Neurotoxicity Research
- Identification and Quantification in Food
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiovascular Conditions and Treatments
- Insect behavior and control techniques
- Genetically Modified Organisms Research
- Viral Infectious Diseases and Gene Expression in Insects
- Weed Control and Herbicide Applications
- Physiological and biochemical adaptations
Brazilian Agricultural Research Corporation
2014-2024
University of Minnesota
2022
Universidade Estadual Paulista (Unesp)
1999-2016
Núcleo de Pesquisas Aplicadas (Brazil)
2015-2016
Natural History Museum
2016
American Museum of Natural History
2014
Serviço Autônomo de Água e Esgoto de Jaboticabal
2011
Faculdade Brasil
2008
Universidade Estadual de Campinas (UNICAMP)
2003-2004
University of North Carolina at Greensboro
1999
Abstract Background Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species due in part to its exceptionally high levels of polyphagy. This also nuisance overwintering human-made structures. It has caused significant agricultural losses recent years along Atlantic seaboard North America and continental Europe. Genomic resources will assist with determining molecular basis for this species’ feeding habitat traits, defining potential targets pest...
DNA methods are useful to identify ingested prey items from the gut of predators, but reliable detection is hampered by low amounts degraded DNA. PCR-based can retrieve minute starting material suffer amplification biases and cross-reactions with predator related species genomes. Here, we use PCR-free direct shotgun sequencing total isolated harlequin ladybird Harmonia axyridis at five time points after feeding on a single pea aphid Acyrthosiphon pisum. Sequence reads were matched three...
Characterizing trophic networks is fundamental to many questions in ecology, but this typically requires painstaking efforts, especially identify the diet of small generalist predators. Several attempts have been devoted develop suitable molecular tools determine predatory interactions through gut content analysis, and challenge has achieve simultaneously high taxonomic breadth resolution. General practical methods are still needed, preferably independent PCR amplification barcodes, recover...
The brown marmorated stink bug, Halyomorpha halys, is a devastating invasive species in the USA. Similar to other insects, olfaction plays an important role its survival and reproduction. As odorant-binding proteins (OBPs) are involved initial semiochemical recognition steps, we used RNA-Sequencing (RNA-Seq) identify OBPs antennae, studied their expression pattern different body parts under stimulation by either aggregation or alarm pheromone food odorants. Thirty full-length putative...
Research on non-target effects of transgenic crop plants has focused primarily bitrophic, tritrophic and indirect entomotoxins from Bacillus thuringiensis, but little work considered intergenerational transfer Cry proteins. This reports a lepidopteran (Chlosyne lacinia) taking up Bt entomotoxin when exposed to sublethal or low concentrations, transferring the eggs, having adverse first filial generation (F1) offspring. Two bioassays were conducted using concentration toxin (100.0 ng/µl...
Abstract Background A central challenge of DNA gut content analysis is to identify prey in a highly degraded community. In this study, we evaluated detection using metabarcoding and method mapping unassembled shotgun reads (Lazaro). Results mock community, did not detect any prey, probably owing primer choice and/or preferential predator amplification, while Lazaro detected with accuracy 43–71%. Gut field-collected arthropod epigeal predators (3 ants, 1 dermapteran, carabid) from...
Olfaction plays a fundamental role in insect survival through resource location and intra interspecific communications. We used RNA-Seq to analyze transcriptomes for odorant-binding proteins (OBPs) from major stink bug pest species Brazil, Euschistus heros, Chinavia ubica, Dichelops melacanthus, their egg parasitoid, Telenomus podisi. identified 23 OBPs E. 25 C. 9 D. 7 T. The deduced amino acid sequences of the full-length had low intraspecific similarity, but very high similarity between...
The boll weevil (Anthonomus grandis Boheman) (Coleoptera: Curculionidae) is an introduced pest in Brazil, which 30 yr has successfully expanded to various eco-regions and became the most important of cotton (Gossypium hirsutum, Malvaceae). Given limited knowledge about adaptive mechanisms that allowed successful establishment population a tropical region, this work we studied potential Midwest weevils enter reproductive dormancy identified importance feeding source for induction dormancy. We...
Plant defense response is an elaborate biochemical process shown to depend on the plant genetic background and biological stressor. This work evaluated soybean foliar brown stink bug herbivory injury through analysis of redox metabolism proteomic 2DE profiles susceptible (BRS Silvania RR) resistant (IAC-100) varieties. The activity lipoxygenase-3, guaiacol peroxidase, catalase ascorbate peroxidase was monitored every 24 h up 96 h. In variety, caused increase in activities lipoxygenase 3 no...
Quantifying species trophic interaction strengths is crucial for understanding community dynamics and has significant implications pest management conservation. DNA-based methods to identify interactions have revolutionized these efforts, but a limitation the poor ability quantify strength of interactions, that biomass or number prey consumed. We present an improved pipeline, called Lazaro, map unassembled shotgun reads comprehensive arthropod mitogenome database show detected quantitatively...
Several methods have been published to estimate per capita predation rates from molecular gut content analysis relying on intuitive understanding of predation, but none formally derived. We provide a theoretical framework for estimating identify an accurate method and lay bare its assumptions. Per can be estimated by multiplying the prey decay rate quantity in predators. This assumes that variation is approximately normally distributed, occurs exponentially, steady state. described several...
The problem of estimating predation rates from molecular gut content data has been challenging. Previous work showed how per capita could be estimated quantitative using the average prey quantity in predator, decay rate predator and a conversion constant to convert measured into numbers or biomass. Based on this previous work, we developed illustrated method estimate relative for single species consumed by one species. This does not require estimation either constant. We describe qPCR,...
Mitogenome sequences are highly desired because they used in several biological disciplines. Their elucidation has been facilitated through the development of massive parallel sequencing, accelerating their deposition public databases. However, assembly and annotation methods might induce variability quality, raising concerns about accuracy that have deposited In this work we show different sequencing (number species pooled a library, insert size platform) generated variable completeness...
The intergenerational transfer of plant defense compounds by aposematic insects is well documented, and since 2006, has been shown for Cry toxins. toxins are proteins naturally produced the soil bacterium Bacillus thuringiensis (Bt) its genes have expressed in plants to confer insect pest resistance. In this work we tested if non-aposematic larvae a major maize pest, Spodoptera frugiperda, with resistance Cry1F, could Cry1F from genetically engineered variety their offspring. Resistant...
Constitutive expression of Odorant-Binding Proteins (OBPs) in antennae and other body parts has been examined mainly to infer their involvement insect olfaction, while regulation response semiochemical stimuli remained poorly known. Previous studies were basically done using electrophysiology, which integrates the set OBPs present an antenna or sensillum, without revealing ones might be involved. In this study we used boll weevil as a model mined its by RNA-Seq simultaneous antennal qPCR...
Abstract: The objective of this work was to determine the survival pattern cotton boll weevil during fallow in Midwestern Brazil. percentage adults that remained reproductive structures, searching for shelters, and longevity fed on pollen nectar as alternative food sources were determined. For this, four populations sampled squares bolls, totaling 11,293 from 2008 2012. emergency monitored collection structures until next season. In laboratory, newly-emerged hibiscus or Spanish needle, their...