A5081216407- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Multiple Myeloma Research and Treatments
- Hematopoietic Stem Cell Transplantation
- Peptidase Inhibition and Analysis
- CAR-T cell therapy research
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Gastroesophageal reflux and treatments
- Phagocytosis and Immune Regulation
- Renal Transplantation Outcomes and Treatments
- MicroRNA in disease regulation
- Biomarkers in Disease Mechanisms
- Gut microbiota and health
- Reproductive tract infections research
- Cervical Cancer and HPV Research
- Acute Myeloid Leukemia Research
- Glycosylation and Glycoproteins Research
- Gastrointestinal motility and disorders
- Phytochemical compounds biological activities
- Helicobacter pylori-related gastroenterology studies
- Circular RNAs in diseases
Aristotle University of Thessaloniki
2018-2023
Centre for Research and Technology Hellas
2019-2023
AHEPA University Hospital
2021
Epigenetic changes, including altered small non-coding RNAs, appear to be implicated in the pathogenesis of sporadic parathyroid adenomas (PAs). In this study, we investigated circular RNAs (circRNAs) expression profile PAs. Sixteen tissue samples PAs, and four normal (NPT) were investigated. Sample preparation microarray hybridization performed based on Arraystar's standard protocols, circRNAs sequences predicted by bioinformatics tools. We identified 35 that differentially expressed PAs...
Background Microenvironmental interactions of the malignant clone with T cells are critical throughout natural history chronic lymphocytic leukemia (CLL). Indeed, clonal expansions and shared clonotypes exist between different CLL patients, strongly implying selection by antigens. Moreover, immunogenic neoepitopes have been isolated from clonotypic B cell receptor immunoglobulin sequences, offering a rationale for immunotherapeutic approaches. Here, we interrogated (TR) gene repertoire...
Abstract Subset #201 is a clinically indolent subgroup of patients with chronic lymphocytic leukemia defined by the expression stereotyped, mutated IGHV4-34/IGLV1-44 BCR Ig. characterized recurrent somatic hypermutations (SHMs) that frequently lead to creation and/or disruption N-glycosylation sites within Ig H and L chain variable domains. To understand relevance this observation, using next-generation sequencing, we studied how SHM shapes subclonal architecture repertoire in subset #201,...
Increasing evidence supports a role for the vaginal microbiome (VM) in severity of HPV infection and its potential link to cervical intraepithelial neoplasia. However, lot remains unclear regarding precise certain bacteria context positivity persistence infection. Here, using next generation sequencing (NGS), we comprehensively profiled VM series 877 women who tested positive at least one high risk (hrHPV) type with COBAS® 4,800 assay, after self-collection cervico-vaginal sample. Starting...
Classification of patients with chronic lymphocytic leukemia (CLL) based on the somatic hypermutation (SHM) status clonotypic immunoglobulin heavy variable (IGHV) gene has established predictive and prognostic relevance. The SHM is assessed number mutations within IG domain sequence, albeit only over rearranged IGHV excluding complementarity determining region 3 (VH CDR3). This may lead to an underestimation actual impact SHM, in fact overlooking most critical for antigen-antibody...
Background: CLL subset #4 is the largest stereotyped in IGHV-mutated (M-CLL). The clonotypic B cell receptor immunoglobulin (BcR IG) #4, encoded by IGHV4-34/IGKV3-20 gene pair, displays long heavy complementarity determining region 3 (VH CDR3), enriched positively charged residues; ubiquitous expression of gamma chain isotype; distinctive imprint somatic hypermutation (SHM), characterized frequent introduction acidic residues and pronounced intraclonal diversification. These features are...
Background:In vivo Class Switch recombination (CSR) has previously been reported for a fraction of patients with chronic lymphocytic leukemia (CLL) expressing unmutated IGHV genes (U-CLL) and high mRNA levels activation-induced cytidine deaminase compared to mutated (M-CLL). This observation is certainly intriguing, however inherently limited due the low-sensitive, Sanger-based approach used immunogenetic characterization. Aims: Here, we sought overcome aforementioned limitation obtain more...