A5102987375- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Cancer-related Molecular Pathways
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Treatments and Studies
- Monoclonal and Polyclonal Antibodies Research
- BRCA gene mutations in cancer
- Advanced Breast Cancer Therapies
- Lung Cancer Treatments and Mutations
- Cancer Treatment and Pharmacology
- Cervical Cancer and HPV Research
- Peptidase Inhibition and Analysis
- Breast Lesions and Carcinomas
- Hedgehog Signaling Pathway Studies
- Growth Hormone and Insulin-like Growth Factors
- Immunotherapy and Immune Responses
- Ovarian cancer diagnosis and treatment
- Cancer Cells and Metastasis
- Hepatocellular Carcinoma Treatment and Prognosis
- Lung Cancer Research Studies
- Metabolism, Diabetes, and Cancer
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
Hellenic Cooperative Oncology Group
2013-2023
Aristotle University of Thessaloniki
2014-2023
Molecular Oncology (United States)
2014
Laboratory of Molecular Genetics
2010
Background The aim of the present study was to investigate efficacy adjuvant dose-dense sequential chemotherapy with epirubicin, paclitaxel, and CMF in subgroups patients high-risk operable breast cancer, according tumor subtypes defined by immunohistochemistry (IHC). Materials Methods Formalin-fixed paraffin-embedded (FFPE) tissue samples from 1,039 participating two phase III trials were centrally assessed micro-arrays IHC for 6 biological markers, that is, estrogen receptor (ER),...
BackgroundThe clinical relevance of mismatch repair (MMR) status in patients with nonmetastatic cancer across tumour types remains unclear. Our goal was to investigate the prognostic role MMR deficiency stage I-III colorectal and endometrial cancer.MethodsPatients tissue available for analysis were identified through Hellenic Cooperative Oncology Group (HeCOG)'s repository. Patients had been referred Departments Medical affiliated HeCOG. protein expression evaluated by immunohistochemistry....
Background-Aim To evaluate the prognostic role of elaborate molecular clusters encompassing cyclin D1, E1, p21, p27 and p53 in context various breast cancer subtypes. Methods Cyclin p53, p21 were evaluated with immunohistochemistry 1077 formalin-fixed paraffin-embedded tissues from patients who had been treated within clinical trials. Jaccard distances computed for markers resulted matrix was used conducting unsupervised hierarchical clustering, order to identify distinct groups correlating...
The polymorphism of codon 72 in the p53 tumour suppressor gene has been associated last decade with risk developing various neoplasias. An influence this on ovarian and endometrial cancer also suggested. We examined genotype frequency archival samples from 56 patients neoplasias 51 Cervical smears 30 healthy, human papillomavirus (HPV)-negative women normal cytology colposcopy, served as control sample. Women neoplasias, especially adenocarcinomas, had Arg/Arg more often than healthy...
The Androgen Receptor (AR) is a potential prognostic marker and therapeutic target in breast cancer. We evaluated AR protein expression high-risk cancer treated the adjuvant setting. Tumors were subtyped into luminal (ER+/PgR±/AR±), molecular apocrine (MAC, [ER-/PgR-/AR+]) hormone receptor negative carcinomas (HR-negative, [ER-/PgR-/AR-]). Subtyping was with respect to prognosis taxane therapy. High histologic grade (p < 0.001) increased proliferation = more often appeared MAC HR-negative...
We explored the clinical significance of tumor genotypes and immunophenotypes in non-metastatic colorectal cancer (CRC).In primary tumors (paraffin blocks) from 412 CRC patients treated with adjuvant chemotherapy, we examined pathogenic mutations (panel NGS; 347 informative); mismatch repair (MMR) immunophenotype (360 CD8+ lymphocyte density (high - low; informative). The outcome measure was disease-free survival (DFS).We evaluated 1713 (median: 3 per tumor; range 0-49); 118/412 (28.6%)...
Epigenetic changes, including altered small non-coding RNAs, appear to be implicated in the pathogenesis of sporadic parathyroid adenomas (PAs). In this study, we investigated circular RNAs (circRNAs) expression profile PAs. Sixteen tissue samples PAs, and four normal (NPT) were investigated. Sample preparation microarray hybridization performed based on Arraystar's standard protocols, circRNAs sequences predicted by bioinformatics tools. We identified 35 that differentially expressed PAs...
Genomic patterns of nasopharyngeal carcinomas (NPCs) have as yet been studied in Southeast Asian (SEA) patients. Here, we investigated genomic locally advanced NPC European (SEE) patients treated with chemoradiotherapy. We examined 126 tumors (89% EBV positive) from Greek and Romanian massively parallel sequencing. Paired tumor‐cell‐rich (TC) infiltrating‐lymphocyte‐rich (TILs) samples were available 19 paired tumor‐germline 68 cases. Top mutated genes BRCA1 (54% all tumors); BRCA2 (29%);...
Dose-dense sequential (dds) chemotherapy has changed the clinical outcome of patients with early breast cancer (BC). To investigate impact dose intensity (DI) in adjuvant setting BC, this observational trial (HE 10/10) was conducted assessing long-term survival outcome, safety and toxicity a currently widely used chemotherapeutic regimen. In addition, prognostic significance tumor infiltrating lymphocytes (TILs) CD8
The aim of this study was to investigate the prognostic value Hedgehog (Gli, Patched-1, Shh, Smo) and Notch (Jag1, Notch2, Notch3) pathway members, in comparison a panel proteins (ER, PgR, HER2/neu, Ki67, p53, p16, PTEN MMR) previously suggested be involved pathogenesis endometrial cancer, association with clinical outcome standard clinicopathological characteristics. A total 204 patients histological diagnosis cancer treated from 2004 2013 were included. evaluation protein expression...
Background: Trastuzumab is a monoclonal antibody against HER2-positive breast cancer. Despite improving the natural history of disease, there number patients who are resistant to it, whereas all will eventually develop resistance and disease progress. Inconsistent preclinical data show that IGF-R pathway may contribute either de novo or acquired trastuzumab. Materials Methods: In total, 227 trastuzumab-treated metastatic cancer were evaluated for IGF-1, IGF-1R, GLP-1R, Akt1, Akt2 Akt3 mRNA...
The aim of the study was to determine importance genetic polymorphisms glutathione-S-transferase T1 and M1 cytochrome P1A1 genes in development cervical intraepithelial neoplasia Greek women. This a prospective, case-control conducted by Cervical Pathology Colposcopy Unit University Ob/Gyn Department from 1999 2003. smears 114 controls without any cytological and/or colposcopical evidence pathology 166 women with history (56 CIN I, 54 II 56 III) were examined polymerase chain reaction for...
Introduction We sought to determine the level of protein expression critical components insulin-like growth factor receptor (IGFR) pathway and evaluate their prognostic significance across different early breast cancer subtypes. Patients Methods Archival tumor tissue from 1,021 women with early, node positive cancer, who were prospectively evaluated within two randomized clinical trials, was used construct microarrays that stained for hormone receptors (HR), Ki67, HER2, epidermal (EGFR)...
The Notch pathway has been implicated in triple-negative breast cancer (TNBC). Herein, we studied the subcellular localization of less investigated Notch2 and Notch3 that Jagged1 (Jag1) ligand patients with operable TNBC.We applied immunohistochemistry for Notch2, Jag1 333 tumors from TNBC treated adjuvant anthracycline-based chemotherapy. We evaluated cytoplasmic (c), membranous (m) nuclear (n) protein localization.c-Notch2 (35% positive tumors), c-Notch3 (63%), c-Jag1 (43%), m-Notch3 (23%)...
Background/Aim: Early-stage colorectal cancer (CRC) carries a wide range of survival probabilities. Novel biomarkers in this setting are eagerly awaited. Cancer stem cells (CSCs) considered one the reasons for treatment failure. This study sought to determine whether activation pathways governing function CSC9s could correlate with outcomes. Materials and Methods: Tumor specimens from 325 patients were analyzed immunohistochemistry (IHC) Hedgehog Notch pathway results correlated prognosis....
The retinoblastoma (RB) gene is a tumor-suppressor that plays central role in regulating the cell cycle. Inactivation of this involved breast cancer.A total 827 patients with cancer treated taxane-based adjuvant chemotherapy were included study. Protein expression RB, phosphorylated RB (pRB), p16, cyclin D1 and p53 was evaluated by immunohistochemistry.Neither markers (RB pRB) reached statistical significance terms their association disease-free or overall survival. Nevertheless, when...
Tumor-infiltrating lymphocytes (TILs) contribute to breast cancer (BC) prognosis. We investigated the prognostic impact of CD8+ TILs in patients with early treated adjuvant chemotherapy a large observational clinical trial. Along 10 year follow-up, considering efficacy and safety, we report results translational part our study. examined patients’ tumors for total (t), stromal (s), intratumoral (i) CD8 lymphocyte density (counts/mm2) on tissue-microarray cores. The counts DFS OS, its...