A5027166388- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Retinal Development and Disorders
- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Wnt/β-catenin signaling in development and cancer
- Cardiomyopathy and Myosin Studies
- Nerve injury and regeneration
- Skin and Cellular Biology Research
- Animal Behavior and Reproduction
- Invertebrate Immune Response Mechanisms
- Alzheimer's disease research and treatments
- Glycogen Storage Diseases and Myoclonus
- Sphingolipid Metabolism and Signaling
- Insect and Arachnid Ecology and Behavior
- Adenosine and Purinergic Signaling
- Connective Tissue Growth Factor Research
École Polytechnique Fédérale de Lausanne
2014-2024
Hôpital de Cery
2014-2016
King Abdullah University of Science and Technology
2014
University Hospital of Lausanne
2014
Howard Hughes Medical Institute
2009
Virginia Commonwealth University
2009
University of Lausanne
2000-2003
Morpho (United States)
2003
Significance The transfer of lactate, a product aerobic glycolysis, from astrocytes to neurons was recently shown be necessary for the establishment long-term memory and maintenance in vivo potentiation. Here, we report that lactate induces expression plasticity genes such as Arc, c-Fos, Zif268 neurons. action is mediated by modulation NMDA receptor activity downstream Erk1/2 signaling cascade, through mechanism associated with changes cellular redox state. These observations unveil an...
Granulocytes generate a "respiratory burst" of NADPH oxidase-dependent superoxide anion (O(2)(-*)) production that is required for efficient clearance bacterial pathogens. Hv1 mediates voltage-gated H(+) channel activity proposed to serve charge-balancing role in granulocytic phagocytes such as neutrophils and eosinophils. Using mice which the gene encoding replaced by beta-Geo reporter protein sequence, we show expression measurable current unstimulated phagocytes. O(2)(-*) substantially...
Neural differentiation, synaptic transmission, and action potential propagation depend on membrane sphingolipids, whose metabolism is tightly regulated. Mutations in the ceramide transporter CERT (CERT1), which involved sphingolipid biosynthesis, are associated with intellectual disability, but pathogenic mechanism remains obscure. Here, we characterize 31 individuals de novo missense variants CERT1. Several fall into a previously uncharacterized dimeric helical domain that enables...
Abstract The decline of neuronal synapses is an established feature ageing accompanied by the diminishment function, and in motor system at least, a reduction behavioural capacity. Here, we have investigated Drosophila neuron synaptic terminals during ageing. We observed cumulative fragmentation presynaptic structures both evoked miniature neurotransmission occurring tandem with reduced ability. Through discrete manipulation each modality, find that but not required to maintain architecture...
Abstract SCG10 is a membrane‐associated, microtubule‐destabilizing protein of neuronal growth cones. Using immunoelectron microscopy, we show that in the developing cortex mice, specifically localized to trans face Golgi complex and apparently associated with vesicular structures putative Consistent this, subcellular fractionation rat forebrain extracts demonstrates enriched fractions containing apparatus cone particles. In isolated particles, was found be particularly concentrated vesicle...
The decline of motor ability is a hallmark feature aging and accompanied by degeneration synaptic terminals. Consistent with this, Drosophila synapses undergo characteristic age-dependent structural fragmentation co-incident diminishing ability. Here, we show that synapse levels Trio, an evolutionarily conserved guanine nucleotide exchange factor (GEF), age. We demonstrate increasing Trio expression in adult can abrogate fragmentation, postpone the ability, maintain capacity to sustain...
The storage and use of glycogen, the main energy reserve in brain, is a metabolic feature astrocytes. Glycogen synthesis regulated by Protein Targeting to (PTG), member specific glycogen-binding subunits protein phosphatase-1 (PPP1). It positively regulates glycogen through de-phosphorylation both synthase (activation) phosphorylase (inactivation). In cultured astrocytes, PTG mRNA levels were previously shown be enhanced neurotransmitter noradrenaline. To achieve further insight into role...
Abstract Alteration of the levels, localization or post-translational processing microtubule associated protein Tau is with many neurodegenerative disorders. Here we develop adult-onset models for human (hTau) toxicity in Drosophila that enable age-dependent quantitative measurement central nervous system synapse loss and axonal degeneration, addition to effects upon lifespan, facilitate evaluation factors may contribute Tau-dependent neurodegeneration. Using these models, interrogate...
Abstract Glutamate is a principal neurotransmitter used extensively by the nervous systems of all vertebrate and invertebrate animals. It primarily an excitatory that has been implicated in system development, as well myriad brain functions from simple transmission information between neurons to more complex aspects function including synaptic plasticity, learning, memory. Identification glutamatergic their sites glutamate release are thus essential for understanding mechanisms neural...
Abstract Within the extracellular matrix, matricellular proteins are dynamically expressed nonstructural that interact with cell surface receptors, growth factors, and proteases, as well structural matrix proteins. The cellular communication network factors family of serve regulatory roles to regulate function defined by their conserved multimodular organization. Here, we characterize expression neuronal requirement for Drosophila factor member. is in nervous system throughout development...
Establishing with precision the quantity and identity of cell types brain is a prerequisite for detailed compendium gene protein expression in central nervous system (CNS). Currently, however, strict quantitation numbers has been achieved only Caenorhabditis elegans . Here, we describe development synergistic pipeline molecular genetic, imaging, computational technologies designed to allow high-throughput, precise cellular resolution reporters intact whole tissues complex constitutions such...
ABSTRACT Establishing with precision the quantity and identity of cell types brain is a prerequisite for detailed compendium gene protein expression in central nervous system. Currently however, strict quantitation numbers has been achieved only system C.elegans . Here we describe development synergistic pipeline molecular genetic, imaging, computational technologies designed to allow high-throughput, precise cellular resolution reporters intact whole tissues complex constitutions such as...
Stathmin/Op18 is a highly conserved 19 kDa cytosolic phosphoprotein. Human and chicken stathmin share 93% identity with only 11 amino acid substitutions. One of the substituted acids serine 25, which glycine in stathmin. In human stathmin, 25 main phosphorylation site for MAP kinase. this study, we have compared The proteins were expressed Sf9 cells using baculovirus expression system purified vitro assays. Phosphorylation kinase showed that was phosphorylated 10 times less than To identify...