Guolong Zuo
A5108894425- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Nicotinic Acetylcholine Receptors Study
- Fullerene Chemistry and Applications
- Atherosclerosis and Cardiovascular Diseases
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Photoreceptor and optogenetics research
University of California, San Francisco
2024-2025
Broad Center
2024-2025
Center for Excellence in Brain Science and Intelligence Technology
2023-2025
University of Chinese Academy of Sciences
2023-2025
University of San Francisco
2024
The development of the human neocortex is highly dynamic, involving complex cellular trajectories controlled by gene regulation1. Here we collected paired single-nucleus chromatin accessibility and transcriptome data from 38 neocortical samples encompassing both prefrontal cortex primary visual cortex. These span five main developmental stages, ranging first trimester to adolescence. In parallel, performed spatial transcriptomic analysis on a subset illustrate organization intercellular...
The development of the human neocortex is a highly dynamic process and involves complex cellular trajectories controlled by cell-type-specific gene regulation1. Here, we collected paired single-nucleus chromatin accessibility transcriptome data from 38 neocortical samples encompassing both prefrontal cortex primary visual cortex. These span five main developmental stages, ranging first trimester to adolescence. In parallel, performed spatial transcriptomic analysis on subset illustrate...
Prenatal nicotine exposure impairs fetal cortical grey matter volume, but the precise cellular mechanisms remain poorly understood. This study elucidates role of nicotinic acetylcholine receptors (nAChRs) in progenitor cells and radial glia (RG) during human development. We identify two nAChR subunits, CHRNA7 human-specific CHRFAM7A expressed SOX2+ progenitors neurons, with particularly enriched along RG endfeet. activation organotypic slices dissociated cultures increases proliferation...
Abstract A comprehensive atlas of genes, cell types, and their spatial distribution across a whole mammalian brain is fundamental for understanding function the brain. Here, using snRNA-seq Stereo-seq techniques, we generated mouse with information 308 clusters single-cell resolution involving over 6 million cells as well 29,655 genes. We have identified new astrocyte clusters, demonstrated that distinct exhibit preference cortical subregions. In addition, 155 genes exhibiting regional...