A5031535803- Genetic Mapping and Diversity in Plants and Animals
- Aquaculture disease management and microbiota
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Aquaculture Nutrition and Growth
- Chromosomal and Genetic Variations
- Physiological and biochemical adaptations
- Immune Response and Inflammation
- interferon and immune responses
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Invertebrate Immune Response Mechanisms
- Molecular Biology Techniques and Applications
- Milk Quality and Mastitis in Dairy Cows
- NF-κB Signaling Pathways
- T-cell and Retrovirus Studies
- Congenital heart defects research
- Fish Ecology and Management Studies
- Plant Disease Resistance and Genetics
- Reproductive biology and impacts on aquatic species
- Wheat and Barley Genetics and Pathology
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Single-cell and spatial transcriptomics
- MicroRNA in disease regulation
Research Institute for Farm Animal Biology (FBN)
2015-2024
University of Rostock
2019-2024
Leibniz Association
1999-2015
Leibniz Institute for Neurobiology
2010-2013
Institute of Molecular Biology
2013
Research Institute of Organic Agriculture
2009
Chungbuk National University
2005
Australian National University
2005
Texas A&M University
2001-2004
Medizinische Hochschule Hannover
2004
ABSTRACT Coordination of the primary defense mechanisms against pathogens relies on appropriate expression pathogen recognition receptors (PRRs) triggering early release effector molecules innate immune system. To analyze impact this system counteraction infections mammary gland (mastitis), we characterized bovine gene encoding key PRR Toll-like receptor 9 (TLR9) and mapped its precise position chromosome BTA22. The sequence information was used to establish real-time PCR quantification...
Several studies in a variety of breeds have reported at least two QTL for milk production traits, including fat synthesis on bovine chromosome 6 (BTA6), comprising region that comparatively has been mapped to equivalent syntenic intervals human, pig, and mouse harboring loci associated with type II diabetes obesity-related traits. We identified the peroxysome proliferator-activated receptor-γ coactivator-1α gene (PPARGC1A) as plausible positional functional candidate previously described...
A second-generation 5000 rad radiation hybrid (RH) map of the cattle genome was constructed primarily using ESTs that were targeted to gaps in existing cattle–human comparative map, as well sparsely populated intervals. total 870 markers added, bringing number mapped on RH panel 1913. Of these, 1463 have significant BLASTN hits (E < e –5 ) against human sequence. created sequence coordinates paired orthologs. One-hundred and ninety-five conserved segments (defined by two or more genes)...
The recently constructed river buffalo whole-genome radiation hybrid panel (BBURH5000) has already been used to generate preliminary (RH) maps for several chromosomes, and buffalo-bovine comparative chromosome have constructed. Here, we present the first-generation whole genome RH map (WG-RH) of generated from cattle-derived markers. aligned bovine sequence assembly Btau_4.0, providing valuable mapping information both species. A total 3990 markers were typed on BBURH5000 panel, which 3072...
The pikeperch (Sander lucioperca) is a fresh and brackish water Percid fish natively inhabiting the northern hemisphere. This species emerging as promising candidate for intensive aquaculture production in Europe. Specific traits like cannibalism, growth rate meat quality require genomics based understanding, an optimal husbandry domestication process. Still, community lacking annotated genome sequence to facilitate genome-wide studies on pikeperch. Here, we report first highly contiguous...
Analyses on the cellular level are indispensable to expand our understanding of complex tissues like mammalian heart. Single-nucleus sequencing (snRNA-seq) allows for exploration composition and cell features without major hurdles single-cell sequencing. We used snRNA-seq investigate first time an entire adult quantification clustering led accurate representation types, revealing 24 distinct clusters with endothelial cells (28.8%), fibroblasts (25.3%), cardiomyocytes (22.8%) constituting...
Water temperature is doubtlessly one of the most important factors affecting well-being fish. For pikeperch Sander lucioperca an ambient water >20 °C has been suggested to achieve optimal growth in aquaculture farms, although natural habitats seldom reach such temperatures. We used multiplex qPCR arrays assess impact between 15 and 25 based on expression 38 potential biomarker genes characterising heat shock hypoxia response, immune activation, energy supply development liver gills...
Stocking density is considered as a key factor determining the productivity of fish aquaculture systems. The transcriptomic response to crowding stress is, however, still poorly investigated. We aimed identify potential biomarker genes via microarray analyses gain insight into molecular pathways modulated by density-induced in farmed rainbow trout Oncorhynchus mykiss. Transcriptome profiling liver, kidney, and gills was complemented analysis classical plasma parameters behavioural...
Abstract The creatine/phosphocreatine system is the principal energy buffer in mammals, but scarcely documented fish. We measured gene expression of major enzymes this system, glycine amidinotransferase (GATM), guanidinoacetate N-methyltransferase (GAMT) and muscle-type creatine kinase (CKM) kidney, liver, muscle tissues fish mammals. CKM was expressed strongly muscles all examined species. In contrast, GATM GAMT were tissue fish, not This indicates that synthesis usage are spatially...
Airbreathing catfish are stenohaline freshwater fish capable of withstanding various environmental conditions and farming practices, including breathing atmospheric oxygen. This unique ability has enabled them to thrive in semi-terrestrial habitats. However, the genomic mechanisms underlying their adaptation adverse ecological environments remain largely unexplored, primarily due limited availability high-quality resources. Here, we present a haplotype-resolved near telomere-to-telomere...
Interval mapping was carried out to identify quantitative trait loci (QTL) for milk production traits in five granddaughter design families of the German Holstein population. Fourteen randomly generated markers spanning whole BTA6 and six targeted microsatellite from BTA6q21–31 were included analysis. In one family a QTL with effects on fat yield protein mapped interval TGLA37‐FBN13 (3 c m proximal FBN13 , lodscore 3 ·22) middle part chromosome. Although there are several reports about...
Abstract Creatine plays an important role in the cell as energy buffer. As system is a basic element of organism it may possibly contribute to differences between rainbow trout strains selected for traits growth and robustness, respectively. The cDNA sequences creatine-related genes encoding glycine amidinotransferase ( GATM ), guanidinoacetate N-methyltransferase GAMT creatine kinase muscle-type CKM) transporter 1 (CT1, encoded by gene solute carrier family 6, member 8 SLC6A8 )) were...
The complex and still poorly understood nature of thermoregulation in various fish species complicates the determination physiological status on basis diagnostic marker genes indicative molecular pathways. present study aimed to compare impacts both gradual acute temperature rise from 18 24°C maraena whitefish aquaculture. Microarray-based transcriptome profiles liver, spleen kidney heat-stressed revealed modulation a significantly higher number those groups exposed gradually rising...