A5051316793- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Advanced Neural Network Applications
- Immune cells in cancer
- Autonomous Vehicle Technology and Safety
- 3D Printing in Biomedical Research
- Cytokine Signaling Pathways and Interactions
- Neonatal and Maternal Infections
- Microfluidic and Bio-sensing Technologies
- Infective Endocarditis Diagnosis and Management
- Oral microbiology and periodontitis research
- Advanced Fluorescence Microscopy Techniques
- T-cell and B-cell Immunology
- Magnetic and Electromagnetic Effects
- Photoreceptor and optogenetics research
- Epigenetics and DNA Methylation
- Innovation in Digital Healthcare Systems
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Advanced Biosensing Techniques and Applications
- Vehicle License Plate Recognition
- Biosensors and Analytical Detection
Chan Zuckerberg Initiative (United States)
2025
Chan Zuckerberg Biohub Chicago
2025
University of Chicago
2019-2025
University of Florida
2010-2018
Streptococcus mutans regulates genetic competence through a complex network that receives inputs from number of environmental stimuli, including two signalling peptides designated as CSP and XIP. The response the downstream genes to these shows evidence stochasticity bistability has been difficult interpret. We have used microfluidic, single-cell methods study how combinations extracellular signals shape comX, an alternative sigma factor governing expression late genes. find composition...
The behavior of the NF-κB pathway as a differentiator circuit enables to track changes in cytokine dose.
Abstract Simultaneous measurement of proteins and mRNA in single cells enables quantitative understanding modeling cellular functions. Here, we present an automated microfluidic system for multi-parameter ultra-sensitive protein/mRNA measurements cells. Our technology improves the sensitivity digital proximity ligation assay by up to 55-fold, with a detection limit 2277 per cell efficiency as few 29 protein molecules. using this reveal higher mRNA/protein correlation mammalian than previous...
Infected cells communicate through secreted signaling molecules like cytokines, which carry information about pathogens. How differences in cytokine secretion affect inflammatory over space and how responding decode from propagating cytokines are not understood. By computationally experimentally studying NF-κB dynamics cocultures of signal-sending (macrophages) signal-receiving (fibroblasts), we find that signals transmitted by wave-like propagation activity create well-defined activation...
Genetic competence in Streptococcus mutans is a transient state that regulated response to multiple environmental inputs. These include extracellular pH and the concentrations of two secreted peptides, designated CSP (competence-stimulating peptide) XIP (comX-inducing peptide). The role cues regulating can be difficult disentangle from effects organism's physiological its chemical modification environment. We used microfluidics control environment study activation key gene comX. find comX...
The capacity to internalize and catabolize carbohydrates is essential for dental caries pathogens persist cause disease. expression of many virulence-related attributes by Streptococcus mutans, an organism strongly associated with human caries, influenced the peptide signaling pathways that control genetic competence. Here, we demonstrate a relationship between efficiency competence carbohydrate source. A significant increase in activity promoters comX, comS, comYA after exposure...
Stimulation of the innate immune system is crucial in both effective vaccinations and immunotherapies. This often achieved through adjuvants, molecules that usually activate pattern recognition receptors (PRRs) stimulate two signaling pathways: nuclear factor kappa-light-chain-enhancer activated B-cells pathway (NF-κB) interferon regulatory factors (IRF). Here, we demonstrate ability to alter improve adjuvant activity via addition small molecule "immunomodulators". By modulating instead...
Streptococcus mutans expresses comX (also known as sigX), which encodes a sigma factor that is required for development of genetic competence, in response to the peptide signals XIP and CSP environmental factors. (sigX inducing peptide) derived from ComS activates unimodally chemically defined media via ComRS system. (competence stimulating bimodally peptide-rich through ComDE two-component However, CSP-ComDE activation indirect involves ComRS. Therefore, bimodality CSP-dependent may arise...
Bacterial pathogens rely on chemical signaling and environmental cues to regulate disease-causing behavior in complex microenvironments. The human pathogen Streptococcus mutans employs a particularly sensing scheme genetic competence other virulence behaviors the oral biofilms it inhabits. Individual S. cells make decision enter competent state by integrating physical received from their microenvironment along with endogenously produced peptide signals. Studies at single-cell level, using...
Many bacterial species use the MecA/ClpCP proteolytic system to block entry into genetic competence. In Streptococcus mutans, degrades ComX (also called SigX), an alternative sigma factor for comY operon and other late competence genes. Although mechanism of has been studied in multiple species, its role within noisy pathways is poorly understood. S. mutans can be triggered by two different peptides, CSP XIP, but it not known whether acts similarly both stimuli, how affects heterogeneity,...
자율주행에서 카메라를 통한 상황인지는 필수 동작 중에 하나이다. 특히 도로주행을 위한 차선인식 시스템은 완전 자율주행 뿐 아니라 ADAS (Advanced Driving Assistance System)를 LKS (Lane Keeping System) 에서도 기능 중 하지만, 이런 도로 위의 다양한 상황들 (광학 반사, 날씨, 밤시간, 가림, 곡선 차선 등)에서 정확하고 실시간으로 동작해야 한다. 본 논문에서는 한국 도로환경에서 밤시간에도 동작할 수 있는 딥러닝 기반 시스템에 대해 고찰하고 성능 최적화를 전략들을 제시하였다. 이를 위해 기존 외국 지형 낮 시간대의 훈련 데이터인 TuSimple 데이터 셋에 더하여 시간대 및 밤 셋을 구축하고 증식 기법들을 추가하여 훈련을 시켰으며 NVIDIA 2080ti 단일 GPU에서 그 정확도 실시간 속도를 측정하였다. 추가 데이터셋 구축 훈련의 결과 Ultra Fast Lane Detection 모델을 기준으로 시간대에 76.9%의...
For autonomous driving, it is imperative to perform various high-computation image recognition tasks with high accuracy, utilizing diverse sensors perceive the surrounding environment. Specifically, cameras are used lane detection, object and segmentation, and, in absence of lidar, extend inferring 3D information through depth estimation, reconstruction, SLAM. However, accurately processing all these operations real-time for driving under constrained hardware conditions practically...
In this paper, lane detection technology used as an essential image recognition for the autonomous driving has been dealt with. particular, possibility of real-time operation during night time in Korean road environments were studied. To end, addition to existing foreign terrain daytime training data, additional was performed by constructing dataset and nighttime environments. As a result with dataset, it possible achieve accuracy improvement 92.4 % from 76.9 roads 10–14 ms latency.
Various sensor-based visual recognition tasks are essential for autonomous driving to perceive the surrounding environment. Using cameras, such as lane detection, object and image segmentation performed. In cases without LiDAR, like 3D distance estimation, restoration, SLAM conducted. However, due hardware constraints demand rapid safe driving, processing all these computations is challenging. this study, a solution achieving under given conditions was investigated by considering accuracy...
Abstract Cells receive a wide range of dynamic signaling inputs during immune regulation, but how gene regulatory networks measure and interpret such is not understood. Here, we used microfluidic live-cell analysis mathematical modeling to study NF-κB pathway in single-cells responds time-varying as increasing, decreasing or fluctuating cytokine signals. Surprisingly, found that acts differentiator, responding strictly the absolute difference concentration, concentration itself. Our analyses...
Abstract Many scenarios in cellular communication requires cells to interpret multiple dynamic signals. It is unclear how exposure immune stimuli alters transcriptional responses subsequent stimulus under inflammatory conditions. Using high-throughput microfluidic live cell analysis, we systematically profiled the NF-κB response different signal sequences single cells. We found that dynamics stores history of signals received by cells: depending on dose and type prior pathogenic cytokine...
Summary Infected cells communicate through secreted signaling molecules like cytokines, which inform nearby about the type, severity and location of pathogens. How differences in cytokine secretion affect inflammatory over space time, how responding decode information from propagating signals are not understood. By computationally experimentally studying NF-κB dynamics co-cultures signal sending (macrophages) receiving (fibroblasts), we found that transmitted by wave-like propagation...