A5076920776- Digestive system and related health
- Cancer Cells and Metastasis
- Block Copolymer Self-Assembly
- Interconnection Networks and Systems
- Pancreatic function and diabetes
- Polymer Surface Interaction Studies
- 3D Printing in Biomedical Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Parallel Computing and Optimization Techniques
- Embedded Systems Design Techniques
- VLSI and FPGA Design Techniques
- Advanced Polymer Synthesis and Characterization
- Infant Nutrition and Health
- Soil Carbon and Nitrogen Dynamics
- Surfactants and Colloidal Systems
- Diabetes Treatment and Management
- Microbial Community Ecology and Physiology
- Electrostatics and Colloid Interactions
- Lipid Membrane Structure and Behavior
- Material Dynamics and Properties
- Celiac Disease Research and Management
- Airway Management and Intubation Techniques
- Metabolism, Diabetes, and Cancer
- Diabetes and associated disorders
Wuhan No.1 Hospital
2016-2025
University of California, Los Angeles
2001-2023
The Affiliated Yongchuan Hospital of Chongqing Medical University
2023
Chongqing Medical University
2023
Henan Normal University
2023
Chongqing Three Gorges Central Hospital
2023
Alibaba Group (China)
2019-2021
Mattel Children's Hospital
2006-2020
Wuhan Third Hospital
2020
Zhejiang University
2015
Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and required for endocrine-cell development the pancreas intestine. The NEUROG3 gene therefore a candidate cause of newly discovered autosomal recessive disorder characterized by generalized malabsorption paucity enteroendocrine cells.We screened genomic DNA from three unrelated patients with sparse mutations NEUROG3. We then tested ability observed to alter function, using vitro vivo assays.The had few intestinal positive...
The intestinal crypt-niche interaction is thought to be essential the function, maintenance, and proliferation of progenitor stem cells found at bases crypts. These are constantly renewing epithelium by sending differentiated from base crypts Lieberkühn villus tips where they slough off into lumen. niche consists various cell types, extracellular matrix, growth factors surrounds cells. There have recently been advances in understanding interactions that regulate behavior there great interest...
Background: Biochar from pyrolysis of biomass amended in soils to improve nitrogen use efficiency for enhancing crop productivity and mitigate climate change agriculture has been well documented. However, application soil amendment biochar at high rates could be challenged with cost-effectiveness small-scale household farms. Results: This study, by field testing four organic/inorganic compound fertilizers biochars pyrolysed via different biowastes compared conventional chemical fertilizer a...
Intestinal epithelial stem cells (ISCs) are the focus of recent intense study. Current in vitro models rely on supplementation with Wnt agonist R-spondin1 to support robust growth, ISC self-renewal, and differentiation. subepithelial myofibroblasts (ISEMFs) important supportive within niche. We hypothesized that co-culture ISEMF enhances growth ISCs allows for their successful vivo implantation engraftment. ISC-containing small intestinal crypts, FACS-sorted single ISCs, ISEMFs were procured...
Background & Aims Intestinal microfold (M) cells are specialized epithelial that act as gatekeepers of luminal antigens in the intestinal tract. They play a critical role mucosal immune response through transport viruses, bacteria and other particles across epithelium to within Peyer's patch regions sites. Recent studies mice have demonstrated M generated from Lgr5+ stem (ISCs), infection with Salmonella enterica serovar Typhimurium increases cell formation. However, it is not known whether...
Methods for the in vitro culture of primary small intestinal epithelium have improved greatly recent years. A critical barrier translation this methodology to patient's bedside is ability grow stem cells using a well-defined extracellular matrix. Current methods rely on use Matrigel(™), proprietary basement membrane-enriched matrix gel produced mice that not approved clinical use. We demonstrate first time capacity support long-term growth murine monoculture, type I collagen. further...
Microphase separation of binary mixed A/B polymer brushes exposed to different solvents is studied using Single-Chain-in-Mean-Field simulations. Effects solvent quality and selectivity, grafting density, composition, chain-length asymmetry are systematically investigated, diagrams morphologies in various constructed as a function density composition or asymmetry. The structure the microphase segregated lacks long-range periodic order, it analyzed quantitatively Minkowski measures.
Abstract The ability to vary, adjust, and control hydrophobic interactions is crucial in manipulating between biological objects the surface of synthetic materials aqueous environment. To this end a grafted polymer layer (multi‐component mixed brush) synthesized that capable reversibly exposing nanometer‐sized fragments at its hydrophilic tuning, turning on, off interactions. reversible switching occurs response changes environment alters strength range attractive or amphiphilic probes...
The Na + -glucose cotransporter ( SGLT1) is expressed primarily by small intestinal epithelial cells and transports the monosaccharides glucose galactose across apical membrane. Here we describe isolation characterization of 5.3 kb 5′-flanking region SGLT1 gene transiently transfecting reporter constructs into a variety cell lines. A fragment (nt −235 to +22) promoter showed strong activity in line Caco-2 but was inactive nonintestinal (Chinese hamster ovary). Within this region, three...
Microphase separation of A-b-B diblock copolymer brushes in selective solvents is investigated by single-chain-in-mean-field (SCMF) simulations. Depending on the solvent selectivity blocks, fraction (f) A block, and grafting density (σ), perpendicular segregation, lateral or their combination occurs. Using extensive simulations, we obtain morphology diagrams terms f σ for block different solvents. The morphologies nanoscopic domains lack long-range order, they are analyzed using integral...
While microbial nitrogen transformations in soils had been known to be affected by heavy metal pollution, changes abundance and community structure of the mediating populations not yet well characterized polluted rice soils. Here, using prevailing molecular fingerprinting enzyme activity assays comparisons adjacent non-polluted soils, we examined ammonia oxidizing denitrifying communities paddies two sites with different accumulation situation under long-term pollution from mining smelter...
Congenital diarrhea disorders are a group of genetically diverse and typically autosomal recessive that have yet to be well characterized phenotypically or molecularly. Diagnostic assessments generally limited nutritional challenges histologic evaluation, many subjects eventually require prolonged course intravenous nutrition. Here we describe next-generation sequencing techniques investigate child with perplexing congenital malabsorptive other presumably unrelated clinical problems; this...
Abstract Remifentanil-induced hyperalgesia (RIH) is a common clinical phenomenon that limits the use of opioids in pain management. Esketamine, non-competitive N -methyl- d -aspartate (NMDA) receptor antagonist, has been shown to prevent and treat RIH. However, underlying effect mechanism esketamine on RIH remains unclear. This study aimed investigate role preventing treating based NMDA receptor–CaMKIIα pathway. In this study, an experimental animal model was used determine therapeutic...
Taking a nucleation perspective, we study the nature of disordered micelles in highly asymmetric, sphere-forming diblock copolymer melts using self-consistent-field theory. The are shown to correspond strong, activated, localized composition fluctuations state due finite molecular weights. By taking into account translational entropy micelles, obtain concentration and free energy micelles. critical micelle temperature (in terms familiar combination χN), operationally defined onset sufficient...
Fluorescence-activated cell sorting (FACS) is an essential tool for studies requiring isolation of distinct intestinal epithelial populations. Inconsistent or lack reporting the critical parameters associated with FACS methodologies has complicated interpretation, comparison, and reproduction important findings. To address this problem a comprehensive multicenter study was designed to develop guidelines that limit experimental data variability provide foundation accurate comparison between...
Numerous genes expressed by intestinal epithelial cells are developmentally regulated, and the influence that adaptive (AI) passive (PI) immunity have in controlling their expression has not been evaluated. In this study, we tested hypothesis both PI AI influenced enterocyte gene developing a breeding scheme used T B cell-deficient recombination-activating (RAG) mice. RNA was isolated from liver proximal/distal small intestine at various ages, steady-state levels of six different transcripts...