A5068776649- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Renal cell carcinoma treatment
- Diet and metabolism studies
- 3D Printing in Biomedical Research
- Digestive system and related health
- Amino Acid Enzymes and Metabolism
- Renal and related cancers
- Glutathione Transferases and Polymorphisms
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Prostate Cancer Diagnosis and Treatment
- Genetics, Aging, and Longevity in Model Organisms
- Sexual function and dysfunction studies
- Prostate Cancer Treatment and Research
- Urinary Bladder and Prostate Research
- Gastrointestinal Tumor Research and Treatment
- Urinary Tract Infections Management
- Multiple Myeloma Research and Treatments
- Proteoglycans and glycosaminoglycans research
- Bioinformatics and Genomic Networks
Tulane University
2023-2024
University of Alabama at Birmingham
2017-2024
Mattel Children's Hospital
2014-2016
University of California, Los Angeles
2013-2016
The heparan sulfate-degrading enzyme heparanase promotes the progression of many cancers by driving tumor cell proliferation, metastasis and angiogenesis. Heparanase accomplishes this via multiple mechanisms including its recently described effect on enhancing biogenesis exosomes. Because we discovered that expression is upregulated in myeloma cells survive chemotherapy, were prompted to investigate impact anti-myeloma drugs exosome biogenesis. When exposed commonly utilized bortezomib,...
Background We previously reported in vitro maintenance and proliferation of human small intestinal epithelium using Matrigel, a proprietary basement membrane product. There are concerns over the applicability Matrigel-based methods for future therapies. investigated type I collagen as an alternative culture epithelial cells. Methods Human intestine was procured from fresh surgical pathology specimens. Small crypts were isolated EDTA chelation. Intestinal subepithelial myofibroblasts...
Intestinal epithelial stem cells (ISCs) are the focus of recent intense study. Current in vitro models rely on supplementation with Wnt agonist R-spondin1 to support robust growth, ISC self-renewal, and differentiation. subepithelial myofibroblasts (ISEMFs) important supportive within niche. We hypothesized that co-culture ISEMF enhances growth ISCs allows for their successful vivo implantation engraftment. ISC-containing small intestinal crypts, FACS-sorted single ISCs, ISEMFs were procured...
Methods for the in vitro culture of primary small intestinal epithelium have improved greatly recent years. A critical barrier translation this methodology to patient's bedside is ability grow stem cells using a well-defined extracellular matrix. Current methods rely on use Matrigel(™), proprietary basement membrane-enriched matrix gel produced mice that not approved clinical use. We demonstrate first time capacity support long-term growth murine monoculture, type I collagen. further...
Elevation of L-2-hydroxylgutarate (L-2-HG) in renal cell carcinoma (RCC) is due part to reduced expression L-2-HG dehydrogenase (L2HGDH). However, the contribution carcinogenesis and insight into biochemistry targets this small molecule remains be elucidated.Genetic pharmacologic approaches modulate levels were assessed for effects on vitro vivo phenotypes. Metabolomics was used dissect biochemical mechanisms that promote accumulation RCC cells. Transcriptomic analysis utilized identify...
Analysis of transcriptomic data demonstrates extensive epigenetic gene silencing the transcription factor PRDM16 in renal cancer. We show that restoration RCC cells suppresses vivo tumor growth. RNaseq analysis reveals imparts a predominantly repressive effect on transcriptome including suppression encoding semaphorin 5B (SEMA5B). SEMA5B is HIF target highly expressed promotes Functional studies demonstrate PRDM16's properties, mediated by physical interaction with transcriptional...
Tumor cells are known to undergo considerable metabolic reprogramming meet their unique demands and drive tumor growth. At the same time, this may come at a cost with resultant vulnerabilities. The small molecule L-2-hdroxyglutarate (L-2HG) is elevated in most common histology of renal cancer. Similar other oncometabolites, L-2HG has potential profoundly impact gene expression. Here, we demonstrate that remodels amino acid metabolism cancer through combined effects on histone methylation RNA...
Ten-eleven translocation-2 (TET2) is a member of the methylcytosine dioxygenase family enzymes and has been implicated in cancer aging because its role as global epigenetic modifier. TET2 large N-terminal domain catalytic C-terminal region. Previous reports have demonstrated that remains active independently domain. As such, function N terminus this protein poorly characterized. Here, using yeast two-hybrid screening, co-immunoprecipitation, several biochemical assays, we found isoforms...
Mounting evidence points to alterations in mitochondrial metabolism renal cell carcinoma (RCC). However, the mechanisms that regulate TCA cycle RCC remain uncharacterized. Here, we demonstrate loss of enzyme expression is retained metastatic tissues. Moreover, proteomic analysis demonstrates reduced far more pronounced relative other tumor types. Loss correlated with transcription factor PGC-1α, which also lost PGC-1α reexpression cells restores enzymes vitro and vivo leads enhanced glucose...
ABSTRACT L-2-hydroxyglutarate (L-2HG) is an oncometabolite found elevated in renal tumors. However, this molecule might have physiological roles that extend beyond its association with cancer, as L-2HG levels are response to hypoxia and during Drosophila larval development. known be metabolized by dehydrogenase (L2HGDH), loss of L2HGDH leads levels. Despite being highly expressed the kidney, role metabolism has not been explored. Here, we report our findings utilizing a novel CRISPR/Cas9...
The mitochondrial enzyme L-2-hydroxyglutarate dehydrogenase (L2HGDH) regulates the abundance of (L-2HG), a potent signaling metabolite capable influencing chromatin architecture, metabolism, and cell fate decisions. Loss L2hgdh activity in humans induces ectopic L-2HG accumulation, resulting neurodevelopmental defects, altered immune function, enhanced growth clear renal carcinomas. To better understand molecular mechanisms that underlie these disease pathologies, we used fruit fly...
The decision whether to undergo prostate biopsy must be carefully weighed. Nomograms have widely been utilized as risk calculators improve the identification of cancer by weighing several clinical factors. recent inclusion multiparametric magnetic resonance imaging (mpMRI) findings into nomograms has drastically improved their nomogram's accuracy at identifying clinically significant cancer. Several novel incorporated mpMRI aid in decision-making process proceeding with a patients who are...
ABSTRACT The Warburg effect is one of most-well studied metabolic phenomenon in cancer cells. For the most part, these studies have focused on enhanced rates glycolysis observed various models. presumption has been that mitochondrial metabolism suppressed. However, recent indicate extent far more heterogeneous tumors than originally presumed. One tumor type with suppression renal cell carcinoma (RCC). Prior suppressed TCA cycle enzyme mRNA expression associated aggressive RCC. Yet,...
Abstract Introduction An iteration of artificial intelligence (AI), large language models (LLMs), such as ChatGPT, have recently gained popularity a potential educational resource for patients, students, and researchers. LLMs utilize deep learning algorithms to predict the next word in sequence based on data library 300 billion words. Recent studies shown that while provide useful information, they are limited due producing false nonsensical information. A focus has been utility ChatGPT an...
You have accessJournal of UrologySexual Function/Dysfunction: Peyronie's Disease (MP28)1 May 2024MP28-14 NOVEL IN VIVO PEYRONIE'S PATIENT-DERIVED XENOGRAFT MODEL RECAPITULATES HUMAN CURVATURE IMMUNOCOMPROMISED RATS Garrett Brinkley, Jacob W. Greenberg, Danish Singh, Asim B. Abdel-Mageed, and Wayne J. G. Hellstrom BrinkleyGarrett Brinkley , GreenbergJacob Greenberg SinghDanish Singh Abdel-MageedAsim Abdel-Mageed HellstromWayne View All Author...
ABSTRACT The mitochondrial enzyme L-2-hydroxyglutarate dehydrogenase (L2HGDH) regulates the abundance of (L-2HG), a potent signaling metabolite capable influencing chromatin architecture, metabolism, and cell fate decisions. Loss L2hgdh activity in humans induces ectopic L-2HG accumulation, resulting neurodevelopmental defects, altered immune function, enhanced growth clear renal carcinomas. To better understand molecular mechanisms that underlie these disease pathologies, we used fruit fly...