A5101851126- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- Cancer-related gene regulation
- Viral Infections and Vectors
- Galectins and Cancer Biology
- Circular RNAs in diseases
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- Gallbladder and Bile Duct Disorders
- Platelet Disorders and Treatments
- Gut microbiota and health
- Cervical Cancer and HPV Research
- Immune Cell Function and Interaction
- Prenatal Screening and Diagnostics
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Complement system in diseases
- Peptidase Inhibition and Analysis
- Genital Health and Disease
- Biomarkers in Disease Mechanisms
Shandong Provincial Hospital
2022-2025
Shandong University
2009-2025
Sun Yat-sen University
2025
Qilu Hospital of Shandong University
2008-2021
Nantong University
2017
University of Louisville
2012
Institute of Immunology
2009
T-cell immunoglobulin- and mucin domain-3-containing molecule 3 (TIM-3) is a membrane protein expressed in various kinds of immune cells plays pivotal role regulation. Recently, TIM-3 was reported to be aberrantly melanoma cells, contributing the low adhesion ability tumor promoting survival cells. We investigated expression non-small cell lung cancers (NSCLCs), further analyzed whether aberrant related prognosis for patients with cancer. Tumor tissue samples from 30 NSCLC were involved....
Syncytin-1 is a protein coded by human endogenous retrovirus (HERV) gene of the HERV-W family (HERVWE1). Syncytin- 1 mediates formation syncytiotrophoblasts through fusion cytotrophoblasts, hallmark terminal differentiation placental trophoblast linage. also possesses nonfusogenic functions and regulates cell cycle progression. While decreased syncytin-1 expression syncytium deficiency are considered important pathological changes in preeclampsia, molecular mechanism(s) underlying...
We investigated the number of intratumoral tumor-infiltrating lymphocytes (TILs), including CD4(+), CD8(+), and CD25(+) T cells, in nonsmall cell lung cancers (NSCLC) their correlation with patient survival time. Tumor specimens from 30 NSCLC patients were consecutively obtained during surgery. Patient status was monitored. Based on time, divided into 2 groups: 5-year group nonsurvival group. cells that infiltrated tumors detected counted by immunohistochemistry. Patients a lower TILs CD8(+)...
Colorectal cancer (CRC) is the third most common malignancy in world. The risk of death closely correlated to stage CRC at time primary diagnosis. Therefore, there a compelling need for identification blood biomarkers that can enable early detection CRC. We used quantitative proteomic approach with isobaric labeling (iTRAQ) examine changes plasma proteome 10 patients compared healthy volunteers. Enzyme-Linked Immunosorbnent Assay (ELISA) and Western blot were further validation. In our...
Bmi-1 is overexpressed in uterine cervical cancer (UCC) and found to be associated with adverse clinical characteristics poor prognosis. However, little information available on the status of circulating mRNA UCC. Because cell-free nucleic acids have emerged as a novel class markers for detection, our research aims address this question by detecting assess its diagnostic prognostic potential Reverse transcription quantitative real-time PCR method was established detect plasma 109 patients...
To evaluate the balance of interleukin IL18 and its endogenous antagonist binding protein (IL18BP) in patients with idiopathic thrombocytopenic purpura (ITP), plasma IL18, IL18BP, interferon gamma (IFNG) IL4 levels, as well platelet counts were measured active ITP (n = 23), remission 21) healthy subjects 24) by enzyme linked immunosorbent assay (ELISA). Using real-time quantitative polymerase chain reaction, mRNA expression IFNG, IL4, T-box (TBX21) GATA-binding 3(GATA3) studied all subjects....
Abstract Syncytin 1 is considered as an oncogene in various malignant tumors, but its effect on non‐small cell lung cancer (NSCLC) has not been reported. We investigated the specific role of NSCLC through transfection knockdown or overexpression plamids A549 cells. Our results proved that inhibited proliferation, and blocked cycle G1 phase by inhibiting expression Nusap1, Cyclin D1, CDK6, CDK4. Cell arrest also leaded to increased apoptosis Suppression migration invasion, well expressions...
Syncytin-1 is a human endogenous retroviral (HERVW) envelope protein, which has been implicated in trophoblast and cancer cell fusions as well immunomodulatory functions. We investigated syncytin-1 expression promoter methylation non-small lung (NSCLC) the adjacent, para-carcinoma tissues. In addition, correlation to patient survival differentiation of between 5-year death group was analyzed.Survival ratio calculated by Kaplan-Meier curve. Death risk assessment executed Cox regression model....
Since March 2020, the pandemic caused by SARS-CoV-2 has affected nearly all aspects of daily life. In this study, we investigated age-stratified prevalence and genotype distribution human papillomavirus (HPV) among females in Shandong province (eastern China) aimed to provide guidance on HPV-based cervical cancer screening vaccination. The HPV genotypes was analyzed using PCR-Reverse Dot Hybridization. overall infection rate 16.4%, which dominated high-risk genotypes. most prevalent HPV16...
Cervical cancer(CCa) remains a significant global public health concern, with early diagnosis and treatment being crucial. Moreover, the molecular mechanisms underlying its pathogenesis remain incompletely elucidated. F2RL1 is closely associated various tumors. However, relationship CCa poorly understood. We accessed data from 309 patients diagnosed TCGA database. The Limma package facilitated differential expression analysis to identify differentially expressed mRNAs (DEmRNAs). Survival ROC...
The current diagnostic methods for biliary tract cancer (BTC) have limitations in sensitivity and specificity. This study aims to explore the use of volatile organic compounds (VOCs) serum distinguish BTC benign diseases (BBD). We collected 158 samples from BBD patients, used gas chromatography ion mobility spectrometry (GC-IMS) VOCs detection. Six machine learning (RF, SVM, LDA, KNN, LASSO, XGBoost) were construct evaluate prediction models. detected a total 40 which 14 statistically...
Abstract Background Long noncoding RNAs (lncRNAs) OGFRP1 is up-regulated in endometrial cancer and cervical carcinoma, suppression inhibits the malignant behavior of cells. Here, we evaluated expression pattern, biological function potential mechanism non-small cell lung (NSCLC). Methods The target genes 25 pairs clinically collected NSCLC normal tissue samples was detected by qRT-PCR or western blot. We screened siRNA (siOGFRP1) to down-regulate A549 H1299 cells were examined CCK8, wound...
This study aimed to investigate the expression and clinical significance of syncytin-1 in serum exosomes hepatocellular carcinoma (HCC) patients. Serum samples were collected from 61 patients with newly diagnosed HCC healthy individuals. Exosomes extracted identified using transmission electron microscopy Western blot. The relative levels determined by real-time quantitative PCR. protein alpha-fetoprotein detected enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed...
The expression of T-cell immunoglobulin domain, mucin domain-3 (Tim-3) in unexplained recurrent spontaneous abortion (URSA) was investigated. Tim-3 mRNA peripheral blood mononuclear cells (PBMCs) URSA and control groups assayed by fluorescent quantitative real-time polymerase chain reaction. protein intensity localization placental villi uterine decidua were determined using immunohistochemical assay. CD4+Tim-3+/CD4+ cell ratio PBMCs flow cytometry. significantly higher than normal controls...
Background Recent studies have revealed changes in microbiota constitution and metabolites associated with tumor progression, however, no causal relation between or diffuse large B-cell lymphoma (DLBCL) has yet been reported. Methods We download a dataset from the MiBioGen study, Canadian Longitudinal Study on Aging (CLSA) DLBCL Integrative Epidemiology Unit Open genome-wide association study (GWAS) project. Mendelian randomization (MR) analysis was conducted using R packages, TwoSampleMR...
<title>Abstract</title> <bold>Background</bold>: Bile circulating free DNA (cfDNA) as a novel liquid biopsy method for the early clinical diagnosis of cholangiocarcinoma (CCA) has been less reported. <bold>Methods</bold>: We collected bile samples from 51 patients with CCA and 48 gallstones at Shandong Provincial third Hospital September 2022 to December 2023. quantified cfDNA syncytin-1 SLC7A11,calculated correlation bySpearman rank correlation, plotted Receiver Operating Characteristic...
BackgroundCervical cancer is one of the most common malignancies in women worldwide. As a RING type ubiquitin ligase, SIAH2 has been reported to promote progression variety tumors by interacting with and targeting multiple chaperones substrates. The aim this study was further identify role related molecular mechanisms involved cervical carcinogenesis.Methods ResultsCellular assays vitro showed that knockdown inhibited proliferation, migration invasion human cells C33A SiHa, induced...
The exact triggers of gallstone formation remain incompletely understood, but research indicates that microbial infection is a significant factor and can interfere with treatment. There no consensus on the bile culture profiles in previous studies, determining profile could aid targeted prevention primary aim this study to investigate differences communities cultured from specimens patients gallstones.
Background: Liquid biopsy has been receiving attention as an emerging detection technology in the clinical application of non-small-cell lung cancer (NSCLC). Methods: We quantified serum circulating free DNA (cfDNA) syncytin-1 126 patients and 106 controls, analyzed correlation level with pathological parameters explored diagnostic utility. Results: The cfDNA levels NSCLC were higher than healthy controls (p < 0.0001). These associated smoking history = 0.0393). area under curve was 0.802,...