A5064042195- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolites in Food Biotechnology
- T-cell and B-cell Immunology
- Biological Research and Disease Studies
- Advanced Proteomics Techniques and Applications
- Chemical Synthesis and Analysis
- Cell Adhesion Molecules Research
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Genomics and Phylogenetic Studies
- Mosquito-borne diseases and control
- Chronic Lymphocytic Leukemia Research
- Proteoglycans and glycosaminoglycans research
University of Maryland, College Park
2016-2021
Johns Hopkins Medicine
2019
Johns Hopkins University
2018-2019
University of Maryland, Baltimore
2012
University of Amsterdam
1990
The fine structures of Fc N-glycans can modulate the effector functions IgG antibodies. It has been demonstrated that lack core fucose on leads to drastic enhancement antibody-dependent cellular cytotoxicity (ADCC), while terminal α2,6-sialylation glycan plays a critical role for anti-inflammatory activity human intravenous immunoglobulin (IVIG). We describe in this paper highly efficient chemoenzymatic method site-selective glycoengineering intact monoclonal antibody and IVIG. Two new...
Abstract CTLA-4 is an important regulator of T-cell function. Here, we report that expression this immune-regulator in mouse B-1a cells has a critical function maintaining self-tolerance by regulating these early-developing B express repertoire enriched for auto-reactivity. Selective deletion from results mice spontaneously develop autoantibodies, T follicular helper (Tfh) and germinal centers (GCs) the spleen, autoimmune pathology later life. This impaired immune homeostasis cell...
Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement M6P ligand-receptor recognition has not been fully understood due difficulties in obtaining homogeneous M6P-containing glycoproteins. We describe here a chemoenzymatic synthesis phosphoglycoproteins carrying natural N-glycans. The method includes chemical glycan oxazolines with varied...
A convergent chemoenzymatic approach for sequential installation of different N-glycans in a polypeptide is described. The method includes introduction distinguishably protected GlcNAc-Asn building blocks during automated solid phase peptide synthesis (SPPS), followed by orthogonal deprotection the GlcNAc primers and site-selective extension sugar chains through glycosynthase-catalyzed transglycosylation reactions. It was observed that protecting groups on one neighboring moiety have an...
A class of new glycan-reactive broadly neutralizing antibodies represented by PGT121, 10–1074, and PGT128 has recently been discovered that targets specific N-glycans the peptide region around V3 domain. However, glycan specificity fine epitopes these bNAbs remain to be further defined. We report here a systematic chemoenzymatic synthesis homogeneous glycopeptides derived from HIV-1 JR-FL strain carrying defined at N332, N301, N295 sites. Antibody binding studies revealed both nature site...
High-mannose-type N-glycans are an important component of neutralizing epitopes on HIV-1 envelope glycoprotein gp120. They also serve as signals for protein folding, trafficking, and degradation in quality control. A number lectins antibodies recognize high-mannose-type N-glycans, glycan array technology has provided avenue to probe these oligomannose-specific proteins. We describe this paper a top-down chemoenzymatic approach synthesize library high-mannose related neoglycoproteins...
Collagen is a potent agonist for platelet activation, presenting itself as key contributor to coagulation via interactions with glycoproteins. The fine details dictating platelet–collagen are poorly understood. In particular, glycosylation could be determinant in the interaction. Here, we report an affinity purification coupled mass spectrometry-based approach elucidate function of N-glycans interactions. By integrative proteomic and glycoproteomic analysis collagen–platelet interactive...
Synthetic bi- and trivalent HIV V3 glycopeptides show a significantly enhanced affinity for HIV-neutralizing antibody 10-1074.
The broadly neutralizing antibody PG9 recognizes a unique glycopeptide epitope in the V1V2 domain of HIV-1 gp120 envelope glycoprotein. present study describes design, synthesis, and antibody-binding analysis glycopeptide-Qβ conjugates as mimic proposed PG9. glycopeptides were synthesized using highly efficient chemoenzymatic method. alkyne-tagged then conjugated to recombinant bacteriophage (Qβ), virus-like nanoparticle, through click reaction. Antibody-binding indicated that synthetic...
Pepsinogen A (PGA) isozymogen patterns in urine and gastric mucosa can be visualised non-denatured polyacrylamide gel electrophoresis by showing proteolytic activity after the conversion of pepsinogen into pepsin acid. This method is not suitable for visualising PGA serum due to low concentrations. To obtain a more sensitive visualisation an immunoblotting technique was developed. from sonified specimens obtained were identical with those staining. The immunostaining at least 50 times...
Abstract Collagen is a potent agonist for platelet activation, presenting itself as key contributor to coagulation via interactions with glycoproteins. The fine-details dictating platelet-collagen are poorly understood. In particular, glycosylation could be determinant in the interaction. Here we report an affinity purification coupled mass spectrometry-based approach elucidate function of N-glycans interactions. By integrative proteomic and glycoproteomic analysis collagen-platelet...
Abstract It has been previously demonstrated that glycogenes can function as important pleiotropic regulators of tumorigenesis. In this study, we investigated the endoplasmic reticulum mannosyl-oligosaccharide 1, 2-alpha-mannosidase (MAN1B1) on behavior prostate cancer cells and found overexpression MAN1B1 in androgen-independent PC3 reduced cellular migration. We extended analysis to an androgen dependent cell line, LNCaP, another independent LNCaP-AI, observing a similar migratory...