A5111494406- Nanoparticle-Based Drug Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- Avian ecology and behavior
- Polymer Surface Interaction Studies
- Photochemistry and Electron Transfer Studies
- Mass Spectrometry Techniques and Applications
- Molecular Junctions and Nanostructures
- Lipid Membrane Structure and Behavior
- Photoacoustic and Ultrasonic Imaging
- Analytical Chemistry and Sensors
- RNA Interference and Gene Delivery
- Protein Interaction Studies and Fluorescence Analysis
- Bird parasitology and diseases
- Fish Ecology and Management Studies
- Luminescence and Fluorescent Materials
- Erythrocyte Function and Pathophysiology
- Advanced Polymer Synthesis and Characterization
- Advanced Fluorescence Microscopy Techniques
- Supramolecular Self-Assembly in Materials
- Nanoplatforms for cancer theranostics
- Characterization and Applications of Magnetic Nanoparticles
- Advanced biosensing and bioanalysis techniques
- Digitalization and Economic Development in Agriculture
- Psychosomatic Disorders and Their Treatments
- Protein Structure and Dynamics
UNSW Sydney
2015-2025
ARC Centre of Excellence in Advanced Molecular Imaging
2014-2024
The University of Sydney
2022-2023
National Imaging Facility
2015-2021
Western University
2013-2017
Children's Cancer Institute Australia
2012-2014
Occupational Cancer Research Centre
2014
University of Strathclyde
2006-2008
Amyloid-β (Aβ) toxicity in Alzheimer's disease (AD) is considered to be mediated by phosphorylated tau protein. In contrast, we found that, at least early disease, site-specific phosphorylation of inhibited Aβ toxicity. This specific was the neuronal p38 mitogen-activated protein kinase p38γ and interfered with postsynaptic excitotoxic signaling complexes engaged Aβ. Accordingly, depletion exacerbated circuit aberrations, cognitive deficits, premature lethality a mouse model AD, whereas...
We describe the synthesis of iron oxide nanoparticles (IONPs) with excellent colloidal stability in both water and serum, imparted by carefully designed grafted polymer shells. The shells were built attached aldehyde functionality to enable reversible attachment doxorubicin (DOX) via imine bonds, providing a controlled release mechanism for DOX acidic environments. IONPs shown be readily taken up cell lines (MCF-7 breast cancer cells H1299 lung cells), intracellular was proven using vitro...
The human mechanosensitive ion channel PIEZO1 is gated by membrane tension and regulates essential biological processes such as vascular development erythrocyte volume homeostasis. Currently, little known about plasma localization organization. Using a PIEZO1-GFP fusion protein, we investigated whether cholesterol enrichment or depletion methyl-β-cyclodextrin (MBCD) disruption of organization dynasore affects PIEZO1-GFP’s response to mechanical force. Electrophysiological recordings in the...
Conferring biodegradability to nanoparticles is vitally important when nanomedicine applications are being targeted, as this prevents potential problems with bioaccumulation of byproducts after delivery. In work, dextran has been modified (and rendered hydrophobic) by partial acetalation. A solid state NMR method was first developed fully characterize the acetalated polymers. a subsequent synthetic step, RAFT functionality attached via residual unmodified hydroxyl groups. The groups were...
It is often assumed that a hydrophobic drug will be entrapped in the environment of micelle. Little attention usually drawn to actual location and effect on properties. In this publication, we show how chosen curcumin not only unexpectedly located shell micelle but also accumulation hydrophilic block can lead changes morphology during self-assembly. A copolymer poly(1-O-methacryloyl-β-d-fructopyranose)-b-poly(methyl methacrylate), poly(1-O-MAFru)36-b-PMMA192, was loaded with different...
ABSTRACT Flying birds depend on their feathers to undertake most activities, and maintain them in peak condition through periodic molt frequent preening. Even small exposures crude oil reduce the integrity of feathers, could impair flight performance. We trained wild western sandpipers (Calidris mauri) perform endurance flights a wind tunnel, used magnetic resonance body composition analysis measure energy expenditure after were exposed weathered MC252 from Deepwater Horizon spill. The cost...
Polymeric micelles have been extensively studied as vectors for the delivery of hydrophobic drugs treatment cancers and other diseases. Despite intensive research, few formulations provide significant benefits, even fewer clinically approved. While many traditional non-responsive excellent safety profiles, they lack ability to respond intracellular environment release their cargo in a spatiotemporally defined manner effectively deliver large doses cytotoxic into cytosol cells that overwhelm...
A novel theranostic controlled drug delivery platform that binds the to nanocarrier by utilising Schiff base bonds achieve high spatial and temporal control over release.
Developmental conditions may influence many aspects of adult phenotype, including growth and immune function. Whether poor developmental environments impair both function or induce a trade-off between the two processes is inconclusive, impact timing stress in determining this relationship has so far been overlooked. We tested hypothesis that long-term effects nutritional on growth, body composition, zebra finches (Taeniopygia guttata) are different depending whether experienced during an...
Abstract Single chain nanoparticles (SCNPs) prepared using diphenylalanine ( L FF) as crosslinker have recently been proposed protein‐inspired drug delivery carriers. The aim of this work is to learn more about the stability these peptide‐based supramolecular systems in biological environments Förster resonance energy transfer and fluorescence lifetime imaging microscopy (FRET‐FLIM). Two self‐folding poly( N,N ‐dimethyl acrylamide) PDMA with 10 mol.% pendant FF, labeled at omega end cyanine...
Summary Migratory birds have been implicated in the spread of some zoonotic diseases, but how well infected individuals can fly remains poorly understood. We used western sandpipers Calidris mauri to experimentally test whether flight is affected when long-distance migrants are mounting an immune response and maintain defences during a wind tunnel. measured five indicators innate immunity ‘flown-healthy’ (flying tunnel without response),’flown-sick’ while acute phase response, which part...
Abstract Empowered by reticular chemistry, an ocu‐metal‐organic framework (MOF) catalyst has been created for the first time. The catalyst, SYD‐1, was rationally designed and successfully synthesised assembling 8‐c prismatic zirconium oxo‐clusters 6‐c octahedral Ru(bpy) 3 2+ ‐based metalloligands into a three‐dimensional structure. Catalytically active metal sites were then attached to coordinatively unsaturated on Zr postsynthetically via sequential practice of solvent‐assisted ligand...
Cationic polysaccharides have been extensively studied for drug delivery via the bloodstream, yet few progressed to clinical use. Endothelial cells lining blood vessel wall are coated in an anionic extracellular matrix called glycocalyx. However, we do not fully comprehend charged polysaccharide interactions with We reveal that cationic poly(acetyl, arginyl) glucosamine (PAAG) exhibits highest association endothelial glycocalyx, followed by dextran (neutral) and hyaluronan (anionic)....
Previously synthesized poly(methacrylic acid-co-cholesteryl methacrylate) P(MAA-co-CMA) copolymers were examined as potential drug delivery vehicles. fluorescently labelled and imaged in SHEP HepG2 cells. To understand their cell internalization pathway endocytic inhibition studies conducted. It was concluded that are taken up by the cells via clathrin-independent endocytosis (CIE) (both caveolae mediated cholesterol dependent endocytosis) mechanisms. The formation characterization of...
Angiogenesis plays a crucial role in development, wound healing as well tumour growth and metastasis. Although the general implication of cytoskeleton angiogenesis has been partially unravelled, little is known about specific actin isoforms this process. Herein, we aimed at deciphering function γ-actin angiogenesis.Localization β- vascular endothelial cells was investigated by co-immunofluorescence staining using monoclonal antibodies, followed functional analysis siRNA. The impact knockdown...
Myelin comprises a compactly stacked massive surface area of protein-poor thick membrane that insulates axons to allow fast signal propagation. Increasing levels the myelin protein plasmolipin (PLLP) were correlated with post-natal myelination; however, its function is unknown. Here, intracellular localization and dynamics PLLP characterized in primary glial cultured cells using fluorescently labeled antibodies against PLLP. localized recycled between plasma Golgi complex. In complex, forms...
Abstract Choroidal melanocytes (HCMs) are melanin-producing cells in the vascular uvea of human eye (iris, ciliary body and choroid). These cranial neural crest-derived migrate to populate a mesodermal microenvironment, display cellular functions extracellular interactions that biologically distinct skin melanocytes. HCMs (and melanins) important normal physiology with roles including photoprotection, regulation oxidative damage immune responses. To extend knowledge cytoplasmic melanins...
Surface functionalization of superparamagnetic iron oxide nanoparticles (IONPs) was achieved by exploiting a grafting "onto" approach simultaneously with an in situ modification the graft block copolymer. Terminal phosphonic-acid-bearing copolymers composed pendant-activated ester moieties, that is, poly(pentafluorophenyl acrylate) (P(PFPA)) and poly(oligoethylene glycol (P(OEGA)), were synthesized assembled on IONP surfaces. The assembly performed presence different primary amines to...
The authors describe the characteristics and application of a 265nm AlGaN light-emitting diode (LED) operated at 1MHz repetition rate, 1.2ns pulse duration, 1.32μW average power, 2.3mW peak ∼12nm bandwidth. LED enables fluorescence decay weakly emitting phenylalanine to be measured routinely, even in dilute solution. For pH 6–9.2, find evidence for biexponential rather than monoexponential decay, providing direct presence rotamers with photophysics closer other two fluorescent amino acids...
Nitrogen vacancy (NV) centers in fluorescent nanodiamonds (FNDs) draw widespread attention as quantum sensors due to their room-temperature luminescence, exceptional photo- and chemical stability, biocompatibility. For bioscience applications, NV FNDs offer high-spatial-resolution capabilities that are unparalleled by other solid-state nanoparticle emitters. On the hand, pursuits further improve optical properties of have reached a bottleneck, with intense debate literature over which many...
A perylene (donor–dimer)–acceptor–(donor–dimer) pentamer array is synthesized to investigate the competition between excimer formation and Förster resonance energy transfer. Using time-resolved fluorescence, we show that, upon excitation, isolated dimer forms an with a time constant of 4.3 ns. However, in array, when either two constituent dimers donate their acceptor fluorophore, trap eliminated. The macromolecule shows broad absorption reduced self-absorption, at some cost fluorescence...