A5057144402- Ocular Oncology and Treatments
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Cutaneous Melanoma Detection and Management
- Multiple Myeloma Research and Treatments
- Ocular Diseases and Behçet’s Syndrome
- melanin and skin pigmentation
- Ubiquitin and proteasome pathways
- Ocular Infections and Treatments
- Lymphoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Retinal and Optic Conditions
- CNS Lymphoma Diagnosis and Treatment
- Retinal Imaging and Analysis
- Systemic Lupus Erythematosus Research
- Ocular Surface and Contact Lens
- Ocular Disorders and Treatments
- Cancer-related Molecular Pathways
- Amino Acid Enzymes and Metabolism
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Nonmelanoma Skin Cancer Studies
- Heavy Metal Exposure and Toxicity
- Intraocular Surgery and Lenses
St Vincent's Hospital Sydney
2016-2025
UNSW Sydney
2009-2024
The University of Notre Dame Australia
2024
St Vincent's Clinic
2019-2024
St Vincent's Hospital
2017-2024
St. Vincent's Birmingham
2022
St. Vincent's Hospital
2022
The Kinghorn Cancer Centre
2019-2022
Saint Vincent's Catholic Medical Center
2022
The University of Sydney
2006-2021
To determine the sub-macular Bruch's membrane (BrM) macrophage count and choroidal BrM immunophenotype in normal eyes with early advanced age-related macular degeneration (AMD).BrM macrophages were counted 125 human (normal, aged, AMD geographical atrophy), CD68 inducible nitric oxide synthase (iNOS) immunohistochemistry was performed on 16 AMD, atrophy disciform scarring). All examined clinically ante mortem. Results correlated histopathological features, including basal laminar deposit...
To correlate basal laminar deposit (BLamD) and membranous debris, including linear (BLinD), with the evolution of early age-related macular degeneration (AMD).A clinicopathologic collection 132 eyes a continuous layer BLamD was reviewed. The thickness type sites debris deposition were correlated clinical progression disease.Two types BLamD, termed late, identified based on light microscopic appearance by using picro-Mallory stain. progressive accumulation late well increasing thickness,...
The human macula is more susceptible than the peripheral retina to developing blinding conditions such as age-related macular degeneration, diabetic retinopathy. A key difference between them may be nature of their Müller cells. We found primary cultured cells from and display significant morphological transcriptomic differences. Macular expressed phosphoglycerate dehydrogenase (PHGDH, a rate-limiting enzyme in serine synthesis) synthesis, glycolytic mitochondrial function were activated...
Cuticular drusen (CD) have been associated with manifestations of age-related macular degeneration such as atrophy and neovascularization in the macula. In this study, eyes CD were followed investigated for estimated 5-year risk progression to sequelae geographic (GA) (MNV).
Objective Toxic metals are suspected to play a role in the pathogenesis of age-related macular degeneration. However, difficulties detecting presence multiple toxic within intact human retina, and separating primary metal toxicity from secondary uptake damaged tissue, have hindered progress this field. We therefore looked for several posterior segment normal adult eyes using elemental bioimaging. Methods Paraffin sections eye seven tissue donors (age range 54–74 years) an bank were examined...
Summary Primary vitreoretinal lymphoma (PVRL) is a high‐grade extranodal non‐Hodgkin lymphoma, with limited prospective data to inform practice. High rates of central nervous system (CNS) relapse contribute its poor prognosis. This international multicentre retrospective cohort study aimed characterise real‐world contemporary practice and outcomes in PVRL (2010–2022). Sixty patients were included from 11 centres across Australia, Singapore, Canada the United Kingdom. Most had systemic...
Experimental evidence suggests human Müller glia exhibit neural progenitor properties in vitro. CD117 and CD44 are known to be expressed by stem cells, the survival of which appears depend critically on interactions with hyaluronan-rich extracellular matrix (ECM). Here, we characterise expression normal adult retina describe how it correlates hyaluronan distribution ocular ECM. By using chromogen-based immunohistochemistry, was found entire cytoplasm spanning from inner outer limiting...
Damage to the retina and optic nerve is found in some neurodegenerative disorders, but it unclear whether pathway central nervous system (CNS) are affected by same injurious agent, or damage due retrograde degeneration following CNS damage. Finding an environmental agent that could be responsible for would support hypothesis this toxicant also triggers lesions. Toxic metals have been implicated mercury has of experimentally-exposed animals. Therefore, see if exposure prenatal period one link...
A bstract Background: To compare the retinal autoantibody profile in individuals with and without early age‐related macular degeneration (AMD) to determine whether baseline autoantibodies are associated progression advanced AMD 5 10 years later. Methods: Western immunoblotting was used detect presence of serum 47 16 healthy controls from Blue Mountains Eye Study. Autoantibody isotype, IgG subclass distribution target antigens group were compared control group. The association between later...
<h3>Aims</h3> To describe subretinal debris found on ultrastructural examination in an eye with macular telangiectasia (MacTel) type 2 and optical coherence tomography (OCT) a subset of patients MacTel 2. <h3>Methods</h3> Blocks from the mid-periphery temporal perifovea clinically documented were examined electron microscopy (EM). Cases came Sydney centre project practices authors. <h3>Results</h3> On EM examination, was accumulation degenerate photoreceptor elements space. Despite...
To describe the clinicopathological features, mutational and chromosomal copy number analysis, 8-year follow-up of a case bilateral diffuse uveal melanocytic proliferation (BDUMP) associated with clear-cell carcinoma endometrium.Histological evaluation, multiplex ligation-dependent probe amplification (MLPA) analysis GNAQ/11 were performed in 67-year-old female patient diagnosis BDUMP.Histological evaluation revealed bland spindle cells, diffusely replacing tract, which showed index less...
Abstract Choroidal melanocytes (HCMs) are melanin-producing cells in the vascular uvea of human eye (iris, ciliary body and choroid). These cranial neural crest-derived migrate to populate a mesodermal microenvironment, display cellular functions extracellular interactions that biologically distinct skin melanocytes. HCMs (and melanins) important normal physiology with roles including photoprotection, regulation oxidative damage immune responses. To extend knowledge cytoplasmic melanins...
Several nomenclature and grading systems have been proposed for conjunctival melanocytic intraepithelial lesions (C-MIL). The fourth "WHO Classification of Eye Tumors" (WHO-EYE04) a C-MIL classification, capturing the progression noninvasive neoplastic melanocytes from low- to high-grade lesions, onto melanoma in situ (MIS), then invasive melanoma. This proposal was revised WHO-EYE05 system, which simplified C-MIL, whereby MIS subsumed into C-MIL. Our aim validate system using digitized...
To report the results of surgery in a patient with Niemann-Pick disease type B, bilateral macular halos, and full-thickness hole (FTMH) right eye.
9510 Background: Darovasertib is a protein kinase C (PKC) inhibitor with meaningful activity in metastatic uveal melanoma (UM) due to its effect on PKC delta downstream of canonical GNAQ/GNA11 mutations. To date, clinical patients localized primary disease has not been assessed either neoadjuvant or adjuvant settings. Methods: Patients planned for enucleation UM were treated an initial safety cohort darovasertib 300mg BID 1 month (n=3 patients), and then following DSMB agreement expansion up...
Routine examination of entire histological slides at cellular resolution poses a significant if not insurmountable challenge to human observers. However, high-resolution data such as the distribution proteins in tissues, e.g., those obtained following immunochemical staining, are highly desirable. Our present study extends applicability PathoFusion framework level. We illustrate our approach using detection CD276 immunoreactive cells glioblastoma an example. Following automatic...
To determine the expression of histone deacetylase enzymes in uveal melanoma tumour cells.This is an observational immunohistochemical study 16 formalin-fixed, paraffin-embedded eyes enucleated for between January 2001 and March 2002. Haematoxylin eosin paraffin sections were reviewed histopathological parameters according to American Joint Committee on Cancer 7th edition. Sections then immunohistochemically stained deacetylases 1, 2, 3, 4 6 sirtuin 2 using automated Leica Bond II platform...
We have developed a platform, termed PathoFusion, which is an integrated system for marking, training, and recognition of pathological features in whole-slide tissue sections. The platform uses bifocal convolutional neural network (BCNN) designed to simultaneously capture both index contextual feature information from shorter longer image tiles, respectively. This analogous how microscopist pathology works, identifying cancerous morphological the context using first narrow then wider focus,...
Invasive fungal sinusitis causes painful orbital apex syndrome with ophthalmoplegia and visual loss; the mechanism is unclear. We report an immunocompromised patient invasive in whom loss was due to posterior ischaemic optic neuropathy, shown on diffusion-weighted MRI, presumably from invasion of small meningeal-based arteries at apex. After intensive antifungal drugs, exenteration immune reconstitution, survived, but we were uncertain if helped. suggest that evidence acute neuropathy should...