The CBC count and leukocyte differential (LDC) are among the most frequently requested clinical laboratory tests. These analyses highly automated, correct interpretation of results requires extensive knowledge analytic performance instruments significance they provide. In this review, we analyze state art regarding traditional new parameters with emphasis on applications quality. problems some count, such as platelet counts, components LDC monocyte basophil other commonly used indices red...

The evaluation of nutritional status is one the primary components multidimensional geriatric assessment. We investigated relationship between some markers malnutrition and modifications in functional a sample older disabled residents living nursing homes.Ninety-eight subjects who were independent at least two activities daily (ADLs) enrolled 2-year longitudinal study. Anthropometric, nutritional, metabolic parameters, as well body composition, measured baseline after 2 years.Deteriorating...

To diagnose iron deficiency in patients undergoing hemodialysis, the percentage of hypochromic RBCs (with cellular hemoglobin concentration <280 g/L [HYPO%]) and mean reticulocyte content (CHret) provided by Siemens ADVIA 120 2120 analyzers (Siemens Diagnostic Solutions, Tarrytown, NY) were proposed as alternatives to biochemical tests. Sysmex, with its XE-5000 analyzer (Sysmex, Kobe, Japan), also erythrocytes lower than 17 pg (%Hypo-He) equivalent (Ret-He) similar clinical applications. Our...

Iron deficiency represents the most frequent cause of anemia. To diagnose iron some biochemical tests such as serum ferritin and transferring saturation percent (TSAT%) are usually used. Recently, hematological parameters mean reticulocyte hemoglobin content (CHr or Ret-He) percentage hypochromic RBCs (Hypo% %Hypo-He) were proposed alternative to tests. In this study, analytic performance diagnostic efficiency these two provided by Sysmex XE5000 analyzer on patients with without anemia (IDA...

The authors evaluated the performance of four modern, commercially available hematology analyzers for imprecision and inaccuracy in determining leukocyte differential count. evaluation was performed according to International Committee Standardization Haematology protocols National Clinical Laboratory Standards H20-T standard, using same group patients simultaneously. Imprecision very low among all neutrophils lymphocytes (coefficient variation maximum = 4.12%), whereas other populations it...

To verify their clinical usefulness in diagnosis and early response to therapy of sideropenic anemia, we compared the behavior reticulocyte parameter (RET-Y), a raw measure dependent on size content cell, generated by Sysmex XE 2100, with mean volume (MCVr) hemoglobin (CHr) from Bayer ADVIA 120 healthy subjects patients iron deficiency anemia. Correlations were high (r = 0.88 r 0.94, respectively). All parameters varied significantly as 48 hours after start intravenous (mean differences...

We performed a parallel evaluation of 5 automated reticulocyte analyzers. The guidelines were those proposed by the National Committee for Clinical Laboratory Standards and International Council Standardisation in Haematology. Duplicate analyses 225 healthy subjects 115 patients affected various diseases. reference intervals different each method (ADVIA 120, 27-125 x 103/μL [27-125 109/L]; CELL DYN 4000, 25-108 [25-108 GEN-S, 20-85 [20-85 SE 9500 RET, 23-95 [23-95 VEGA RETIC, 30-130 [30-130...

To verify their clinical usefulness in diagnosis and early response to therapy of sideropenic anemia, we compared the behavior reticulocyte parameter (RET-Y), a raw measure dependent on size content cell, generated by Sysmex XE 2100, with mean volume (MCVr) hemoglobin (CHr) from Bayer ADVIA 120 healthy subjects patients iron deficiency anemia. Correlations were high (r = 0.88 r 0.94, respectively). All parameters varied significantly as 48 hours after start intravenous (mean differences...

Abstract Introduction In this study, analytic performance (imprecision, carryover, time stability) and diagnostic efficiency of Mindray BC‐6800 analyzer to quantify reticulocytes extended reticulocyte parameters was evaluated. Moreover, reference intervals on adult population were determined. Results compared with those obtained by Sysmex XE‐5000 analyzer. Methods One hundred eighty‐four healthy adults both sexes, 368 subjects affected various pathologic conditions (nutritional anemias...

We performed a parallel evaluation of 5 automated reticulocyte counters to produce the immature fraction (IRF). analyzed 225 samples from healthy control subjects, 115 patients with various diseases, 38 advanced aplasia, and 22 in early erythropoietic recovery after chemotherapy or bone marrow transplantation. The reference intervals were different for each instrument (ADVIA 120, 0.04-0.25; CELL DYN 4000, 0.15-0.35; GEN-S, 0.20-0.37; SE 9500 RET, 0.05-0.21; VEGA RETIC: 0.06-0.23)....

Abstract Background: The study endpoint was to analyze the effect of preanalytical (time, temperature, anticoagulant) and analytical (imprecision, correlation between volume platelet concentration) variables on mean (MPV). A further aim calculate in an adult population reference intervals using Sysmex XE-5000 analyzer. critical evaluation also made clinical utility these parameters. Methods: Analyses above values were performed duplicate 170 healthy adults both sexes: (1) within 30 min from...

We evaluated the performance (ie, imprecision, inaccuracy, and analytic sensitivity) of Sysmex SE-9000 commercial hematology analyzer (TOA Medical Electronics, Kobe, Japan) on differential leukocyte counts according to National Committee for Clinical Laboratory Standards H20-A protocol. The results obtained were compared with those from Bayer H6000 H3 (Bayer Diagnostic Division, Tarrytown, NY), Coulter MAXM (Miami, Fla), microscopic method. Altogether, samples 462 subjects analyzed. show a...

In this study the ability of Coulter MAXM analyzer to quantify reticulocytes was evaluated. The results were compared with those obtained by a microscopic method according NCCLS H44-P recommendations and from automated Sysmex R-1000. Duplicate samples 330 patients analyzed. reference intervals three methods were: 0.37-1.80%, median 0.83%; manual 0.40-2.30%, 1.00%; R-1000 0.60-1.95%, 1.06%. imprecision (CV) at all concentrations is lower than (low 16.1% vs 67%; normal 16.9% 28.9%; high 9.5%...