A5037691658- Pluripotent Stem Cells Research
- Biofuel production and bioconversion
- Lignin and Wood Chemistry
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Ionic liquids properties and applications
- Thermochemical Biomass Conversion Processes
- CRISPR and Genetic Engineering
- Carbon Dioxide Capture Technologies
- Osteoarthritis Treatment and Mechanisms
- Catalysis for Biomass Conversion
- Advanced biosensing and bioanalysis techniques
- Silk-based biomaterials and applications
- Mesenchymal stem cell research
- CO2 Reduction Techniques and Catalysts
- Educational Reforms and Innovations
- Carbon dioxide utilization in catalysis
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Proteoglycans and glycosaminoglycans research
- Supramolecular Self-Assembly in Materials
Shenzhen Institutes of Advanced Technology
2025
Shenzhen University
2025
Anhui University of Technology
2017-2023
Zhengzhou University
2023
Institute of Process Engineering
2011-2021
Northwestern Polytechnical University
2020
Chinese Academy of Sciences
2011-2018
University of Oxford
2003-2014
Science Oxford
2003-2014
Institute of Biomedical Science
2014
Abstract Optical density (OD) is widely used to estimate the of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and challenging relate actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, highly accessible OD protocols across 244 laboratories, applied eight strains constitutive GFP-expressing E. coli . Based on our results, we recommend calibrating estimated count using serial...
Abstract Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails protect a prerequisite developing efficient and successful methods hES lines banks. We investigated stimuli from result in apoptosis, which causes low The level of reactive oxygen species (ROS) significantly increased, F‐actin content...
Abstract In cartilage tissue engineering, the determination of most appropriate cell/tissue culture conditions to maximize extracellular matrix synthesis is major importance. The pH plays an important role in affecting energy metabolism and by chondrocytes. this study, chondrocytes were isolated from bovine articular cartilage, embedded agarose gel, cultured at varied levels (7.3–6.6). Rate lactate production, total glycosaminoglycan (GAG) collagen synthesis, as well cell numbers viability...
Breast cancer is one of the most common cancers. Oridonin, a traditional Chinese medicine, believed to inhibit tumor growth, but its particular effects on breast remain unknown. In this study, we examined oridonin's 4T1, MCF-7, and MDAMB-231 cellular activity using CCK8. Scratch assays were used detect migration. Oridonin's cell cycle studied flow cytometry, expression related proteins p53, CDK2, p21 was detected Western blot assays. Metabolomics changes in small molecule metabolites...
A two-dimensional silicon nanosheet (2D Si NS) is promising as a lithium-ion battery anode. However, insufficient cycling life at high current density hampers its practical applications due to easy fragileness. Rationally engineering the micro/nanostructure address this issue. Unfortunately, precise construction of dedicated into 2D NS meets serious challenges. Herein, facile strategy developed synthesize sandwich-like honeycomb NS/solid NS/honeycomb (h/s/h-Si anode through self-assembled...
Abstract Articular cartilage has a limited capacity for self‐repair after damage. Engineered is promising treatment to replace or repair damaged tissue. The growth of engineered sensitive the extracellular culture environment. Chondrocytes were seeded into alginate beads and agarose scaffolds at 4 millions/mL, response static perfusion was examined over period up 12 days. For both types scaffolds, chondrocytes kept their differentiated morphology days in all conditions. In beads, more...
Abstract Due to widespread applications of human embryonic stem (hES) cells, it is essential establish effective protocols for cryopreservation and subsequent culture hES cells improve cell recovery. We have developed a new protocol dissociated culture. examined the effects formula freezing solution containing 7.5% dimethylsulfoxide (DMSO) (v/v %) 2.5% polyethylene glycol (PEG) (w/v on survival recovery after cryopreservation, further investigated role combination Rho‐associated kinase...
X-linked adrenoleukodystrophy (X-ALD) is a common peroxisomal disorder caused by mutations in the ABCD1 gene, leading to accumulation of very long-chain fatty acids (VLCFAs). This progressive neurodegenerative disease manifests three primary forms: childhood-acquired cerebral demyelination (CALD), adult myelopathy (AMN), and adrenal cortical insufficiency. Bone marrow transplantation effectively halts progression only early stages CALD. A thorough investigation pathophysiology X-ALD has been...
Small peptide ligands which were designed based on the interactions with human immunoglobulin G (IgG) using molecular simulations, can offer a potential alternative for mAb purification elution condition at pH 9 and 3.