A5055777341- Enzyme Structure and Function
- Protein Structure and Dynamics
- Tuberculosis Research and Epidemiology
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Metalloenzymes and iron-sulfur proteins
- Lipid Membrane Structure and Behavior
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- ATP Synthase and ATPases Research
- Machine Learning in Bioinformatics
- Trace Elements in Health
- Carbohydrate Chemistry and Synthesis
- Fibroblast Growth Factor Research
- Biochemical and Structural Characterization
- Hippo pathway signaling and YAP/TAZ
- Chromium effects and bioremediation
- Bioinformatics and Genomic Networks
- Forensic and Genetic Research
Anna University, Chennai
2009-2021
Nitric oxide (NO) promotes angiogenesis by activating endothelial cells. Thalidomide arrests interacting with the NO pathway, but its putative targets are not known. Here, we have attempted to identify these targets.Cell-based assays (wound healing of monolayers and tube formation in ECV304, EAhy926 bovine arterial cells), along ex vivo assays, were used explore interactions between thalidomide NO. We also carried out silico homology modelling docking studies elucidate possible molecular...
purpose. To study some functional candidate genes in cataract families of Indian descent. methods. Nine families, clinically documented to have congenital/childhood cataracts, were screened for mutations such as CRYG (A→D), CRYBB2, and GJA8 by PCR analyses sequencing. Genomic DNA samples either probands or any representative affected member each family amplified sequenced commercially. Documentation single nucleotide polymorphisms (SNPs) was done through BLAST SEARCH...
Pseudomonas aeruginosa lipase is a 29-kDa protein that, following the determination of its crystal structure, was postulated to have lid that stretched between residues 125 and 148. In this paper, using molecular dynamics simulations, we propose there exists, in addition above-mentioned lid, novel second lipase. We further show covering 210-222, acts as triggering for movement first. also investigate role hydrophobicity lids two residues, Phe214 Ala217, play important roles movement. To our...
Mammalian gastric lipases are stable and active under acidic conditions also in the duodenal lumen. There has been considerable interest acid owing to their potential application treatment of pancreatic exocrine insufficiency. In order gain insights into domain movements these enzymes, molecular dynamics simulations human lipase was performed at an pH neutral conditions. For comparative studies, simulation dog pH. Analyses show, that addition lid region, there is another region high mobility...
Abstract Metal ion binding domains are found in proteins that mediate transport, buffering or detoxification of metal ions. In this study, we have performed an silico analysis and identified putative motifs for the ions cadmium, cobalt, zinc, arsenic, mercury, magnesium, manganese, molybdenum nickel. A pattern search against UniProtKB/Swiss-Prot UniProtKB/TrEMBL databases yielded true positives each case showing high-specificity motifs. Motifs were also validated PDB structures site directed...
Abstract Background Proteins having similar functions from different sources can be identified by the occurrence in their sequences, a conserved cluster of amino acids referred to as pattern, motif, signature or fingerprint. The wide usage protein sequence analysis par with growth databases signifies importance using patterns signatures retrieve out related sequences. Blue copper proteins are found electron transport chain prokaryotes and eukaryotes. already existing like type 1 blue,...
Thalidomide is an infamous teratogen and it continuously being explored for its anticancer properties. Fibroblast growth factor receptors (FGFRs) are implicated in embryo development cancer pathophysiology. With striking similarities observed between FGFR conditions thalidomide embryopathy, we hypothesized targets FGFRs. We utilized three different cell lines chicken model to investigate the effects of analogs on expression. performed molecular docking, KINOMEscan analysis, kinase activity...
Abstract Profilin is one of the actin‐binding proteins that regulate dynamics actin polymerization. It plays a key role in cell motility and invasion. also interacts with several other notably through its poly‐L‐proline (PLP) binding site. apicomplexa characterized by unique mini‐domain consisting large β‐hairpin extension an acidic loop which relatively longer Plasmodium species. essential for invasive blood stages falciparum . In current study, unbound profilins from ( Pf ), Toxoplasma...
A novel methodology to predict the local conformational changes in a protein as consequence of missense mutations is proposed. pentapeptide at locus mutation plays dominant role and it analyzed terms tripeptides. measure for spatial temporal fluctuations devised validated. The method does not involve any prior knowledge structural templates from sequence homology studies. Structural deformations can be predicted with about 70–80% reliability protein. Disease causing benign have been...
Metal-ion binding proteins play a vital role in biological processes. Identifying putative metal-ion is through knowledge-based methods. These involve the identification of specific motifs that characterize class protein. have been identified for common metal ions. A robust global fingerprint useful identifying protein from non-metal-ion has not devised. Such method will help novel and do possess canonical motif. We used set physico-chemical parameters encoded by genes CzcA, CzcB CzcD as...