A5013755695- Genomic variations and chromosomal abnormalities
- Genomics and Rare Diseases
- Multicomponent Synthesis of Heterocycles
- Genetics and Neurodevelopmental Disorders
- Synthesis and biological activity
- Prenatal Screening and Diagnostics
- Dermatology and Skin Diseases
- Food Allergy and Anaphylaxis Research
- Neurogenetic and Muscular Disorders Research
- Skin and Cellular Biology Research
- Reproductive System and Pregnancy
- Congenital heart defects research
- Quinazolinone synthesis and applications
- Neurological disorders and treatments
- HIV Research and Treatment
- RNA modifications and cancer
- Synthesis and Biological Evaluation
- Phytase and its Applications
- Pediatric Hepatobiliary Diseases and Treatments
- Neurological diseases and metabolism
- HIV/AIDS drug development and treatment
- Autoimmune Bullous Skin Diseases
- Hemophilia Treatment and Research
- Erythrocyte Function and Pathophysiology
- Transgenic Plants and Applications
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2015-2025
Central Forensic Science Laboratory
2022
Rutgers, The State University of New Jersey
2001-2016
Central Drug Research Institute
2003-2014
Dartmouth–Hitchcock Medical Center
2012
Guru Gobind Singh Indraprastha University
2011
Maulana Azad Medical College
2010
Rutgers New Jersey Medical School
2009
University of Delhi
2001
Université de Montréal
1998
Neonatal sepsis due to intestinal bacterial translocation is a major cause of morbidity and mortality. Understanding microbial colonisation the gut in prematurity may predict risk guide future strategies manipulate microbiome.Prospective longitudinal study premature infants. Stool samples were obtained weekly. DNA was extracted V6 hypervariable region 16S rRNA amplified followed by high throughput pyrosequencing, comparing subjects with without sepsis.Six neonates 24-27 weeks gestation at...
Eukaryotic translation initiation factor eIF5A has been implicated in HIV-1 replication. This protein contains the apparently unique amino acid hypusine that is formed by post-translational modification of a lysine residue catalyzed deoxyhypusine synthase and hydroxylase (DOHH). DOHH activity inhibited two clinically used drugs, topical fungicide ciclopirox systemic medicinal iron chelator deferiprone. Deferiprone reported to inhibit replication tissue culture.Ciclopirox deferiprone blocked...
The first ZnO-NP catalyzed Ugi type three-component (AB2C) reaction has been developed for the synthesis of 2-arylamino-2-phenylacetimidamide from an aldehyde, amine and isocyanide in aqueous media. This nanoparticle catalysed (NPCR) is high yielding good atom economy as well efficiency. synthesized phenylacetimidamide yielded 2-amino-2-phenylacetamide on hydrolysis with I2–SDS–water, whereas alkaline afforded N-substituted α-amino acid.
An efficient micelle promoted one-pot synthesis of 3-amino alkylated indoles has been developed via a three-component Mannich-type reaction from secondary amines, aldehydes and in water under mild conditions. In this reaction, micelles stabilize iminium ions, which undergo nucleophilic addition the indole to give very good yields.
HIV-1 blocks apoptosis, programmed cell death, an innate defense of cells against viral invasion. However, apoptosis can be selectively reactivated in HIV-infected by chemical agents that interfere with gene expression. We studied two globally used medicines, the topical antifungal ciclopirox and iron chelator deferiprone, for their effect on H9 peripheral blood mononuclear infected clinical isolates. Both medicines activated preferentially cells, suggesting drugs mediate escape from...
Antiretrovirals suppress HIV-1 production yet spare the sites of production, DNA-harboring cells that evade immune detection and enable viral resistance on-drug rebound off-drug. Therapeutic ablation pathogenic markedly improves outcome many diseases. We extend this strategy to infection. Using drug-based lead discovery, we report concentration threshold-dependent antiretroviral action medicinal chelator deferiprone validate preclinical findings by a proof-of-concept double-blind trial. In...
Stillbirth is a major global health issue. The fetal autopsy considered one of the primal diagnostic tools to determine cause stillbirth over MRI, ultrasound, and genetic testing. It often way obtain definitive diagnosis can be helpful for families who are grieving loss pregnancy. However, its acceptance limited due cultural, emotional, logistical challenges. This study aimed evaluate role in identifying causative risk factors north India. cross-sectional was performed at tertiary care...
This study aims to evaluate higher-order aberrations (HOAs) pre- and post-cataract surgery using advanced technologies achieve an acceptable best-corrected visual outcome in favor of patients. prospective, observational involves 200 adult patients (aged 40 years or older) undergoing cataract surgery. All these have been evaluated before after surgery.The involved comprehensive measurements both Scheimpflug imaging (Sirius system) ray-tracing technology (iTrace). These were taken...
ABSTRACT Childhood‐onset dystonia, a clinically and genetically diverse group of disorders, can be challenging to diagnose. Information on the genotype phenotype spectrum in Indian population is limited. This study reports clinical molecular findings monogenic childhood‐onset dystonia 22 individuals from 19 families. Complex was most frequent type, followed by combined isolated forms. A total 23 variants across 17 genes were identified, including nine novel ones. These disorders include four...
Many wood-rotting fungi, including Phellinus pomaceus, produce chloromethane (CH3Cl). P. pomaceus can be cultured in undisturbed glucose mycological peptone liquid medium to high amounts of CH3Cl. The biosynthesis CH3Cl is catalyzed by a methyl chloride transferase (MCT), which appears membrane bound. enzyme labile upon removal from its natural location and storage at low temperature bound state. Various detergents failed solubilize the active form, hence it was characterized using fraction....
B(OH)3 catalyzed Ugi three-component reaction for the synthesis of 2-arylamino-2-phenylacetamide has been developed using aldehydes, amines, and isocyanides in water. The synthesized 2-arylamino-2-phenylacetamides were efficiently converted into α-amino acid via acidic hydrolysis.
<b>Background:</b> Atopic dermatitis is a distinct age-related clinical entity. Its etiopathogenesis largely insubstantial. Nevertheless, it seems to be an outcome of interplay maternal and inheritance, pregnancy/intrauterine environmental factors. Besides, immune dysregulation, nutritional supplements also play essential roles. diagnosis has been perpetuated by three or more major/minor criteria. <b>Objectives:</b> An endeavor study its demographic pattern in contemporary prospective....
Mandal, Kausik; Ray, Sayantan; Saxena, Deepti; Srivastava, Priyanka; Moirangthem, Amita; Ranganath, Prajnya; Gupta, Neerja; Mukhopadhyay, Satinath; Kabra, Madhulika; Phadke, Shubha R. Author Information
Abstract Chromosome 5q related Spinal muscular atrophy (SMA) is an autosomal recessive, progressive, neuromuscular disorder most commonly caused by homozygous deletion of exon 7 or and 8 SMN1 gene. Being the leading genetic cause infant mortality, studies its prevalence incidence are necessary. Carrier testing for common pathogenic variant SMA offered to couples visiting our tertiary care hospital in North India. Subjects were tested carrier status Multiplex Ligation‐dependent Probe...
In Saccharomyces cerevisiae, the ability to use proline as a nitrogen source requires Put3p transcriptional regulator, which turns on expression of utilization genes, PUT1 and PUT2, in presence inducer absence preferred sources. Changes target gene occur through an alteration activity DNA-bound Put3p, member Zn(II)2Cys6 binuclear cluster family proteins. Here, we report that 'on' conformation can be mimicked by insertion epitope tag several different places protein, well specific amino acid...
Atopic dermatitis is a well-recognized clinical entity, several facets of which continue to be mystified. Accordingly, its etio-pathogenesis largely elusive. It appears an outcome interplay undertones, namely: genetics, maternal factor and inheritance, pregnancy/intrauterine, environmental factors, immune dysregulation, immuno-globulins, role diet, infection. Besides, recent innovative breakthroughs consisting nutritional supplementation, the highlights were considered worthwhile take stock...
Atopic dermatitis (AD) is an intriguing clinical entity. Its connotations are varied, the updates of which required to be done periodically. An attempt bring its various facets have been made highlighting features keeping in view major and minor criteria facilitate diagnosis, differential complications, associated dermatoses. The benefit current dissertation may percolate trainees dermatology, addition revelations that atopic undertones genetic susceptibility metabolic disorder provide...
Background and Introduction: Chromosomal abnormality is found in about half of first-trimester abortions. Karyotype the gold standard to detect chromosomal abnormalities. Multiplex ligation-dependent probe amplification (MLPA) offers advantage over karyotype terms lower failure rate, faster turnaround time, much higher resolution than conventional karyotyping be 98% concordant with karyotype.We performed this study look for utility MLPA diagnosing abnormalities abortions.MLPA using...
ABSTRACT Treatment of Saccharomyces cerevisiae cells with the immunosuppressive drug rapamycin results in a variety cellular changes response to perceived nutrient deprivation. Among other effects, treatment nuclear localization global nitrogen activators Gln3p and Nil1p/Gat1p, which leads expression assimilation genes. The proline utilization (Put) pathway genes were shown be among induced by rapamycin. Having previously that Put activator Put3p is differentially phosphorylated quality...
Corrosion behavior of iron in hydrochloric acid solution was studied using weight loss as well Scanning electron microscopy study without and with clove oil. The percentage inhibition efficiency increases increasing oil concentration. All the data revel that acts an excellent inhibitor for corrosion HCl solution. Thermodynamic, kinetic parameters equilibrium constant adsorption process were calculated from experimental data. on metals found to follow Langmuir isotherm at all concentration...