A5076594924- Cervical Cancer and HPV Research
- Colorectal and Anal Carcinomas
- Viral-associated cancers and disorders
- Genital Health and Disease
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Colorectal Cancer Screening and Detection
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA and protein synthesis mechanisms
Vrije Universiteit Amsterdam
2018-2024
Amsterdam University Medical Centers
2018-2024
University of Amsterdam
2013-2024
Cancer Center Amsterdam
2022-2024
Dutch Cancer Society
2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2022
Institute of Infection and Immunity
2021
Amsterdam UMC Location University of Amsterdam
2013-2015
Abstract Background High-grade anal intraepithelial neoplasia (HGAIN; AIN2–3) is highly prevalent in HIV+ men, but only a minority of these lesions progress towards cancer. Currently, cancer progression risk cannot be established; therefore, no consensus exists on whether HGAIN should treated. This study aimed to validate previously identified host cell DNA methylation markers for detection and stratification HGAIN. Methods A large independent cross-sectional series 345 cancer, AIN3, AIN2,...
Objective: Anal cancer precursor lesions high-grade anal intraepithelial neoplasia (HGAIN) are highly prevalent among HIV+ MSM. Treatment of HGAIN is frustrated by high recurrence rates. We investigated the efficacy quadrivalent human papillomavirus (qHPV) vaccine as posttreatment adjuvant in preventing Design: Randomized, double-blind, placebo-controlled, multicentre trial. Setting: Three HIV outpatient clinics Amsterdam, Netherlands. Subjects: MSM with CD4 + cell count more than 350...
High-grade anal intraepithelial neoplasia (AIN2/3; HGAIN) is highly prevalent in human immunodeficiency virus positive (HIV+) men who have sex with (MSM), but only a minority will eventually progress to cancer. Currently, the cancer risk cannot be established, and therefore all HGAIN treated, resulting overtreatment. We assessed host cell deoxyribonucleic acid (DNA) methylation markers for detecting Tissue samples of HIV+ (n = 26), AIN3 24), AIN2 42), AIN1 22) male controls 34) were analyzed...
We surveyed trends in incidence (1995-2012) and risk factors for anal cancer the Dutch HIV-positive population. After an initial increase with a peak 2005-2006 of 114 [95% confidence interval (CI): 74 to 169] all HIV+ patients 168 (95% CI: 103 259) men who have sex (MSM), decline 72 43 113) 100 56 164), respectively, was seen 2011-2012. Low nadir CD4, alcohol use, smoking were significantly associated MSM. In conclusion, remains serious problem predominantly However, it seems that rates are...
ABSTRACT Anal squamous cell carcinoma is commonly associated with human papillomavirus (HPV) infection and preceded by low‐ high‐grade anal lesions (LSIL; HSIL). We performed a molecular comparison on paired LSIL‐ HSIL‐lesions collected in longitudinal fashion to assess their relationship. Fifty biopsies from 22 men diagnosed LSIL at baseline (T0) who developed HSIL during follow‐up (T1) were subjected comprehensive analysis: HPV‐typing HPV16 variant, cellular DNA methylation levels, copy...
Anal cancer has increasing incidence and is preceded by high-grade anal intraepithelial neoplasia (HGAIN; AIN2-3). Previously, we identified validated several methylation markers for accurate detection of HGAIN with risk in HIV-positive (HIV+) men who have sex (MSM). This study aimed to evaluate these HIV-negative groups. A cross-sectional series 176 tissue samples cancer, AIN3, AIN2, AIN1 control biopsies obtained women was tested six (ASCL1, LHX8, SST, WDR17, ZIC1 ZNF582). Accuracy AIN3...
Abstract Human papillomavirus (HPV)‐induced anal intraepithelial neoplasia (AIN, graded 1‐3) is highly prevalent in HIV‐positive (HIV+) men who have sex with (MSM), but only a minority of lesions progresses to cancer. Our study aimed characterise comprehensively tissue samples from cross‐sectional series (n = 104) HIV+ MSM and longitudinal 40) AIN2/3 progressing cancer using different biomarkers. The consisted 8 normal, 26 AIN1, 45 AIN2, 15 AIN3 10 squamous cell carcinoma. Tissue sections...
Shallow whole genome sequencing (Shallow-seq) is used to determine the copy number aberrations (CNA) in tissue samples and circulating tumor DNA. However, costs of NGS challenges small biopsies ask for an alternative untargeted approaches. The mFAST-SeqS approach, relying on LINE-1 repeat amplification, showed a good correlation with Shallow-seq detect CNA blood samples. In present study, we evaluated whether suitable assess formalin-fixed paraffin-embedded (FFPE) specimens, using vulva anal...
Introduction Anal cancer precursors, or high-grade anal intraepithelial neoplasia (HGAIN), are highly prevalent in HIV-seropositive (HIV+) men who have sex with (MSM). Around 30% of lesions regress within 1 year, but current histopathological assessment is unable to distinguish between HGAIN likely and persist progress cancer. We aim assess if host cell DNA methylation markers can predict regression HGAIN, thus determining the need for immediate treatment active surveillance. This could...
Abstract Introduction High-resolution anoscopy (HRA) to prevent anal cancer is complex and screening capacity limited. Previously, we showed that DNA methylation analysis of high-grade squamous intraepithelial lesions (HSIL) biopsies can distinguish between HSIL with an increased risk, a low in which treatment may be safely withheld. Here, assessed the performance swabs identify patients underlying risk. Methods A cross-sectional series paired 215 persons HIV 19 were tested for 6 markers....
Background Anal cancer incidence has been increasing in the combined antiretroviral therapy (cART) era HIV+ patients. In this study we surveyed trends anal between 1995 and 2012, analysed a range of potential risk factors. Methods: We retrieved data for all patients diagnosed with from Dutch HIV observational cohort (ATHENA) database. Incidence rates were calculated per 100 000 person-years follow-up (FU), whole period 18 years, 2-year blocks. Potential factors uni- multivariable Cox...