A5033778353- Mesenchymal stem cell research
- Esophageal Cancer Research and Treatment
- Gastric Cancer Management and Outcomes
- Esophageal and GI Pathology
- Reconstructive Facial Surgery Techniques
- Tissue Engineering and Regenerative Medicine
- Metastasis and carcinoma case studies
- Animal Behavior and Welfare Studies
- Facial Rejuvenation and Surgery Techniques
- Body Contouring and Surgery
- Reconstructive Surgery and Microvascular Techniques
- Wound Healing and Treatments
- Orthopedic Surgery and Rehabilitation
- Bone fractures and treatments
- Cancer Cells and Metastasis
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Gastroesophageal reflux and treatments
- Periodontal Regeneration and Treatments
- Eosinophilic Esophagitis
- Adipose Tissue and Metabolism
- Lung Cancer Treatments and Mutations
- Dysphagia Assessment and Management
- Animal Nutrition and Physiology
- Genetic and phenotypic traits in livestock
Ospedale San Carlo
1996-2023
Manchester Metropolitan University
2019-2021
University of Milan
1994-2020
Ospedale San Paolo
1998-2010
University of Padua
1983-2006
AOL (United States)
2005
Azienda Ospedaliera Ospedale Civile di Legnano
1994-1998
Ospedale Maggiore
1994-1997
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
1994-1997
Società Italiana di Cardiologia
1997
Adipose tissue contains multipotent elements with phenotypic and gene expression profiles similar to human mesenchymal stem cells (hMSCs) pericytes. The chance of clinical translation the multilineage potential these is delayed by poor/negligible cell survival within cryopreserved lipoaspirates, difficulty ex vivo expansion, complexity current Good Manufacturing Practice (cGMP) requirements for expanded cells. Hence, availability a minimally manipulated, autologous, hMSC/pericyte-enriched...
Autologous adipose tissue is used for repletion and/or regeneration as an intact lipoaspirate or enzymatically derived stromal vascular fraction (SVF), which may be first cultured into mesenchymal stem cells (MSCs). Alternatively, transplant of autologous mechanically fragmented submillimeter clusters has recently showed remarkable efficacy in diverse therapeutic indications. To document the biologic basis regenerative potential microfragmented tissue, we analyzed distribution perivascular...
Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for advanced cell-based therapies. They routinely obtained enzymatically from fat lipoaspirate (LP) as SVF, and may undergo prolonged ex vivo expansion, with significant senescence decline in multipotency. Besides, these techniques have complex regulatory issues, thus incurring the compelling requirements of GMP guidelines. Hence, availability minimally manipulated, autologous adipose tissue would remarkable biomedical...
Human adipose-derived stem cells (hASCs) have been recently proposed as a suitable tool for regenerative therapies their simple isolation procedure and high proliferative capability in culture. Although hASCs can be committed into different lineages vitro, the differentiation is low-yield often incomplete process. We developed novel nonenzymatic method device, named Lipogems, to obtain fat tissue derivative highly enriched pericytes/mesenchymal by mild mechanical forces from human...
The subcutaneous adipose tissue provides a clear advantage over other mesenchymal stem cell sources due to the ease with which it can be accessed, as well of isolating residing cells. Human adipose-derived cells (hADSCs), localized in stromal–vascular portion, isolated ex vivo using combination washing steps and enzymatic digestion. In this study, we report that microfragmented human lipoaspirated is better source compared normal tissue. structural composition microfragments comparable...
Over the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in closed system device, has been successfully used aesthetic medicine as well orthopedic general surgery. Interestingly, diseases, this ready-to-use cell derivative seems to have prolonged time efficacy even upon single shot injection into osteoarthritic tissues. Here, we investigated...
The anatomic and clinical bases for a reliable versatile homodigital flap covering difficult distal dorsal dorsolateral wounds of the long fingers are presented. This one-step procedure, which uses skin first or second phalanx transposed as reverse island flap, preserving collateral nerve artery to fingertip, has proved valuable in salvaging severely injured digits nine patients (for total 12 flaps). It would seem be relatively simple way achieving early recovery because it does not require...
The anatomic and clinical bases for a reliable versatile homodigital flap covering difficult distal dorsal dorsolateral wounds of the long fingers are presented. This one-step procedure, which uses skin first or second phalanx transposed as reverse island flap, preserving collateral nerve artery to fingertip, has proved valuable in salvaging severely injured digits nine patients (for total 12 flaps). It would seem be relatively simple way achieving early recovery because it does not require...
Research on mesenchymal stem cells from adipose tissue shows promising results for cell-based therapy in cartilage lesions. In these studies, have been isolated, expanded, and differentiated vitro before transplantation into the damaged or onto materials used as scaffolds to deliver impaired area. The present study employed assays investigate potential of intra-articular injection microfragmented lipoaspirate a one-step repair strategy; it aimed determine whether can act scaffold naturally...
Central nervous system (CNS) inflammation is primarily driven by microglial cells which secrete proinflammatory cytokines and undergo proliferation upon activation, as it occurs in neurodegenerative diseases. Uncontrolled or prolonged CNS potentially harmful can result cellular damage. Recently, many studies have focused on human adipose tissue an attractive source of with immunosuppressive properties that modulate inflammation. Our study aimed to evaluate if different methods collection...
Facial feminization surgery (FFS) has recently gained popularity to enhance the female facial profile and promote a real transformation of male face in transgender patients. The term involves overlapping surgical procedures devoted represents dual reversible procedure unique plastic reconstructive surgery. Indeed, FFS envisages modifications hard soft tissues is both reductive augmentative. For these reasons, full (F-FFS) used by surgeons with special expertise FFS. This study describes...
We report the use of a subcuticular purse-string suture for closure surgical skin defects, simple maneuver that we have found to be very useful in closing difficult wounds and reducing scarring. The is performed with large nonabsorbable passed intradermally left situ at least 4 weeks. This technique has been applied 196 patients total 221 sutures over period 2 years, being used close defects from x 8 11 cm many areas body. All showed, closure, number concentric redundant folds as well...
Pig cryptorchidism is a congenital anomaly, which requires surgical intervention. Pain assessment in pig farming has some limitations and consumers are increasingly concerned about suffering linked to on-farm procedures. The PGS seems be promising tool for pain piglets, although it not been investigated whether this applicable growing pigs. This study was designed determine the could useful assess pigs undergoing cryptorchidectomy. Ten mixed-breed cryptorchid were pre-medicated with...
Pericytes (PCs) are mesenchymal stromal cells (MSCs) that function as support and play a role in tissue regeneration and, particular, vascular homeostasis. PCs promote endothelial (ECs) survival which is critical for vessel stabilization, maturation, remodeling. In this study, were isolated from human micro-fragmented adipose (MFAT) obtained fat lipoaspirate characterized NG2+/PDGFRβ+/CD105+ cells. Here, we tested the fat-derived dispensability of CD146 marker with aim better understanding...
Previous research has shown that the transect walks (TW) method provide a practical and effective approach to welfare assessment in broiler turkey farms. This for is reasonable terms of time demands within minimal costs. Furthermore, TW resembles routine checks used by farmers. The overall aim this study was verify feasibility as potential tool on-farm turkeys during fattening period. A total 14 commercial farms (8 male 6 female flocks) same genetic strain (British United Turkeys [B.U.T.] -...
The use of microfragmented adipose tissue (µFAT) for the treatment musculoskeletal disorders, especially osteoarthritis (OA), is gaining popularity, following positive results reported in recent case series and clinical trials. Although these outcomes were postulated to rely on paracrine signals, date, a thorough fingerprint released molecules largely missing. purpose this study was first characterize both structure cell content unprocessed lipoaspirate (LA) µFAT, further identify frame...