A5030824582- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Cancer, Stress, Anesthesia, and Immune Response
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Brain Metastases and Treatment
- NF-κB Signaling Pathways
- Cancer Cells and Metastasis
- Neurogenesis and neuroplasticity mechanisms
- Cancer-related cognitive impairment studies
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Leaf Properties and Growth Measurement
- Neuropeptides and Animal Physiology
- HVDC Systems and Fault Protection
- Tryptophan and brain disorders
Duke University
2021-2025
University of North Carolina at Chapel Hill
2016-2019
Plasmodium falciparum is an obligate human parasite of the phylum Apicomplexa and causative agent most lethal form malaria. Although N6-methyladenosine modification thought to be one major post-transcriptional regulatory mechanisms for stage-specific gene expression in apicomplexan parasites, precise base position m6A mRNAs or noncoding RNAs these parasites remains unknown. Here, we report global nucleotide-resolution mapping residues P. using DART-seq technology, which quantitatively...
The Drosophila polyadenosine RNA binding protein Nab2, which is orthologous to a human lost in form of inherited intellectual disability, controls adult locomotion, axon projection, dendritic arborization, and memory through largely undefined set target RNAs. Here, we show specific role for Nab2 regulating splicing ~150 exons/introns the head transcriptome focus on retention male-specific exon sex determination factor Sex-lethal ( Sxl ) that enriched female neurons. Previous studies have...
Abstract Several recent publications demonstrated that DRD2-targeting antipsychotics such as thioridazine induce proliferation arrest and apoptosis in diverse cancer cell types including those derived from brain, lung, colon, breast. While most studies show 10–20 µM leads to reduced or increased apoptosis, here we lower doses of (1–2 µM) target the self-renewal basal-like breast cells, but not cells other subtypes. We also all lines tested express DRD2 mRNA protein, regardless sensitivity....
Most techniques for mapping m6A-methylated RNAs transcriptome-wide require large amounts of RNA and have been limited to bulk cells tissues. Here, we provide a detailed protocol the identification m6A sites in single HEK293T using single-cell DART-seq (scDART-seq). The details how generate cell lines with inducible expression APOBEC1-YTH transgene use important controls minimizing false positives. We also describe bioinformatic analysis identify sites. For complete on execution this...
Abstract Despite advances in early detection and treatment of breast cancers, metastatic disease remains a significant problem large cause mortality the US. Although tumors are characterized as mass uncontrolled cell division, it is thought that only small populations cells, known tumor initiating cells (TICs), primarily responsible for metastasis. TICs less differentiated than most many show an epithelial-to-mesenchymal transition (EMT). Recently, FDA-approved drug thioridazine, dopamine...
A major mechanism of post-transcriptional RNA regulation in cells is the addition chemical modifications to nucleosides, which contributes nearly every aspect life cycle. N 6 -methyladenosine (m A) a highly prevalent modification cellular mRNAs and non-coding RNAs, it plays important roles control gene expression function. Within brain, proper m critical for neurodevelopment, learning memory, response injury, dysregulation has been implicated variety neurological disorders. Thus,...