A5102860068- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Surface Modification and Superhydrophobicity
- Fluid Dynamics and Heat Transfer
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Iron and Steelmaking Processes
- Gender Roles and Identity Studies
- Eosinophilic Esophagitis
- Protein Kinase Regulation and GTPase Signaling
- Histone Deacetylase Inhibitors Research
- Gastrointestinal Tumor Research and Treatment
- Effects and risks of endocrine disrupting chemicals
- Pancreatic and Hepatic Oncology Research
- ATP Synthase and ATPases Research
- Fire Detection and Safety Systems
- Renal and related cancers
- Melanoma and MAPK Pathways
- Soil, Finite Element Methods
- Cardiac Ischemia and Reperfusion
- Circadian rhythm and melatonin
- Esophageal and GI Pathology
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Genetic and Kidney Cyst Diseases
Sun Yat-sen University
2021-2025
Peking University
2016-2023
China National Center for Food Safety Risk Assessment
2023
State Key Laboratory of Biotherapy
2022
Sichuan University
2022
Zhongshan People's Hospital
2012-2022
The First Affiliated Hospital, Sun Yat-sen University
2021
Creative Commons
2021
Capital University of Economics and Business
2021
Weatherford College
2021
Prime editor (PE), a new genome editing tool, can generate all 12 possible base-to-base conversions, insertion, and deletion of short fragment DNA. PE has the potential to correct majority known human genetic disease-related mutations. Adeno-associated viruses (AAVs), safe vector widely used in clinics, are not capable delivering (∼6.3 kb) single because limited loading capacity (∼4.8 kb). To accommodate AAVs, we constructed four split-PE (split-PE994, split-PE1005, split-PE1024,...
Grb2-associated binder 2 (Gab2), a scaffolding adaptor protein, has recently been implicated in cancer progression. However, the role of Gab2 progression and metastasis colorectal (CRC) remains unclear. expression was assessed CRC patient specimens as well cell lines. Recombinant lentivirus vector containing gene its small interfering RNAs were constructed introduced into cells. Cell migration invasion ability evaluated by transwell assays vitro, vivo performed on nude mice model. Moreover,...
In the current study, based on national fire statistics from 2003 to 2017, we analyzed 24-hour occurrence regularity of in China study and influencing factors provide a reference for scientific effective prevention. The results show that frequency is low 0 6 at night, accounting about 13.48%, but death toll due relatively high, 39.90%. Considering strong seasonal characteristics time series monthly frequency, SARIMA model predicts frequency. According data prediction results, an optimized...
Abstract Mycobacterium tuberculosis , the pathogen of (TB), can survive in host macrophages and induce to M2 phenotype might result latent MTB infection. During phase, expression heat-shock protein 16.3 (Hsp16.3) is markedly increased among most bacterial proteins, but role Hsp16.3 macrophage polarization not clear. In this work, we found that incubated with 100 ng/ml production Arg-1, IL-10, TGF-beta, CD206. These results showed may phenotype. And interaction was depend on chemokine...
Gamma synuclein (SNCG) is a neuronal protein that also aberrantly overexpressed in various types of human cancer. SNCG overexpression promotes cancer invasion and metastasis. However, the mechanisms drive metastasis upon expression remain elusive. Elucidation underlying promotion by may help discover therapeutic avenues for SNCG-overexpressed Here, we show transforming growth factor-β (TGF-β)-induced p38 mitogen-activated kinase (MAPK) phosphorylation. Mechanistically, p38MAPK...
Β-thalassemia is one of the global health burdens. The CD41-42 (-TCTT) mutation at HBB most prevalent pathogenic β-thalassemia in both China and Southeast Asia. Previous studies focused on repairing patient-specific induced pluripotent stem cells, which were subsequently differentiated into hematopoietic progenitor cells (HSPCs) for transplantation. In this study, we directly applied CRISPR/Cas9-based gene editing therapy to correct patient-derived HSPCs. effective by Cas9:sgRNA...
We aimed to investigate the effect of male masturbator on prolonged masturbation duration in general population. 24 single men with normal erectile function participated this experiment. The entire protocol consisted a 4 weeks training using masturbator, corresponding time and round-trip frequency recorded vibration sensor. After training, average assisted increased from 6.00 ± 2.04 min 11.05 4.11 min, an increase about 5 compared that bare hands. However, hands before after has only...
The cytosine base editor (CBE) has shown promise as a gene editing tool for therapy, it can convert cytidine to thymidine. Adeno-associated virus (AAV) been widely used in vivo but its limited 4.7 kb packing capacity presents challenges delivering CBE by single AAV. To address this, one feasible solution is split into two sections dual-AAV delivery. In this study, we utilized BE3 an example and constructed 22 potential split-BE3 pairs with the combination of 11 splitting sites split-inteins...
Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder caused primarily by mutations in the PKD1 gene, resulting formation of numerous cysts and eventually failure. However, there are currently no gene therapy studies aimed at correcting mutations. In this study, we identified two mutation sites associated with ADPKD, c.1198 (C > T) c.8311 (G A), which could potentially be corrected adenine base editor (ABE). The correction efficiencies different ABE variants were tested...
Objective. To analyze the efficacy and safety of submucosal tunnel endoscopic resection (STER) for treatment masses in esophageal muscularis propria. Method. A total 272 patients with propria diagnosed treated our hospital from February 2019 to January 2022 were randomly selected study then divided into STER group ( <math xmlns="http://www.w3.org/1998/Math/MathML" id="M1"> <mi>n</mi> <mo>=</mo> <mn>136</mn> </math> ) mucosal dissection (ESD) id="M2"> according random number table method....
The impact of droplets on solid surfaces is a crucial fluid phenomenon in the additive industry, biotechnology, and chemistry, where controlling dynamics duration essential. While extensive research has focused flat substrates, our understanding curved remains limited. This study seeks to establish phase diagrams for process droplet spheres further quantitatively describe effect curvature through theoretical analysis. It aims determine critical conditions between different outcomes also...
Abstract Rationale Groove pancreatitis (GP) is a rare form of chronic pancreatitis. Since GP presents with nonspecific symptoms, it can be challenging to diagnose. Duodenal obstruction often caused by malignant diseases; however, when associated acute pancreatitis, rarely induced groove Patient's concerns A 56-year-old man who presented complained recurrent upper abdominal discomfort. His concomitant symptoms included pain, postprandial nausea, and vomiting. Contrast-enhanced computed...
Abstract Lanthanum (La) is widely used in modern industry and agriculture because of its unique physicochemical properties broadly exposed the population. Some studies have shown that La may some effects on adipogenesis, but there a lack related vivo evidence. In this study, La(NO 3 ) adipogenesis associated mechanism were studied using C57BL/6J mouse model. The results showed exposure caused decrease body weight percentage fat content mice. addition, adipose marker molecules specific...
This paper presents a comprehensive investigation into the collision dynamics of equal and unequal-sized nanodroplets on flat surface using molecular simulations, revealing new insights scaling laws energy dissipation mechanisms. The conducted with Large-Scale Atomic/Molecular Massively Parallel Simulator software, involved an initially stationary droplet suspended varying diameter ratios (λ) impact velocities. results show that at low Weber numbers (We &lt; 24.15), droplets tend to...
Achalasia (AC) is an esophageal motility disorder clinically manifested as dysphagia.To investigate the effective factors of peroral endoscopic myotomy (POEM) for AC treatment.A total 182 patients treated between August 2019 and September 2020 were enrolled to receive POEM. They assigned group (n = 143) ineffective 39). Their clinical data recorded. The biochemical indices determined.Compared with group, Eckardt score incidence reflux 1 year after operation lower in rate 6 months was higher...
[Objective]To compare the therapeutic effects of different endoscopic interventions on acute nonvariceal upper gastrointestinal bleeding (ANUGB).[Methods]140 patients in our department from September 2009 to 2011 were selected.These divided into three groups depending interventions,50 local spray/injection adrenalin group,50 argon plasma coagulation (APC) group and 40 titanium clips (ETC) group.The clinical 3 analyzed compared.[Results]APC ETC had higher rate initial hemostasis than that (P＜...