A5038847694- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Adipose Tissue and Metabolism
- Plant-Microbe Interactions and Immunity
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- ATP Synthase and ATPases Research
- Silicon Effects in Agriculture
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Machine Learning in Bioinformatics
- Insect-Plant Interactions and Control
- Arsenic contamination and mitigation
- Genomics, phytochemicals, and oxidative stress
- Genetics, Aging, and Longevity in Model Organisms
- Adipokines, Inflammation, and Metabolic Diseases
- Neurobiology and Insect Physiology Research
- Microbial Metabolic Engineering and Bioproduction
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Protein Tyrosine Phosphatases
- Protein Degradation and Inhibitors
University of Missouri
2022-2024
University of Stavanger
2011-2023
University of Calgary
2018-2023
Organogenesis (United States)
2015
Norwegian University of Science and Technology
2014
Mediterranean Agronomic Institute of Chania
2008-2009
Abstract In the postgenomic era, accurate prediction tools are essential for identification of proteomes cell organelles. Prediction methods have been developed peroxisome-targeted proteins in animals and fungi but missing specifically plants. For development a predictor plant carrying peroxisome targeting signals type 1 (PTS1), we assembled more than 2500 homologous sequences, mainly from EST databases. We applied discriminative machine learning approach to derive two different methods,...
Abstract The eukaryotic, highly conserved serine (Ser)/threonine-specific protein phosphatase 2A (PP2A) functions as a heterotrimeric complex composed of catalytic (C), scaffolding (A), and regulatory (B) subunit. In Arabidopsis (Arabidopsis thaliana), five, three, 17 genes encode different C, A, B subunits, respectively. We previously found that subunit, B′θ, localized to peroxisomes due its C-terminal targeting signal Ser-Ser-leucine. This work shows PP2A C2, C5, andA2 subunits interact...
Abstract Background High-accuracy prediction tools are essential in the post-genomic era to define organellar proteomes their full complexity. We recently applied a discriminative machine learning approach predict plant proteins carrying peroxisome targeting signals (PTS) type 1 from genome sequences. For Arabidopsis thaliana 392 gene models were predicted be peroxisome-targeted. The predictions extensively tested vivo , resulting high experimental verification rate of previously not known...
This work reveals information about new peroxisomal targeting signals type 1 and identifies trehalose-6-phosphate phosphatase I as multitargeted is implicated in plant development, reproduction, stress response. A putative, non-canonical signal (PTS1) Pro-Arg-Met > was identified the extreme C-terminus of (TPP)I. TPP catalyzes final step trehalose synthesis, enzyme previously characterized to be nuclear only (Krasensky et al. Antioxid Redox Signal 21(9):1289-1304, 2014). Here we show that...
Protein phosphatase 2A (PP2A) is a heterotrimeric complex comprising catalytic, scaffolding, and regulatory subunit. The subunits are essential for substrate specificity localization of the classified into B/B55, B', B" non-related families in higher plants. In Arabidopsis thaliana, close paralogs B'η, B'θ, B'γ, B'ζ were further subfamily B' called B'η. Here we present results that consolidate evidence role B'η regulation innate immunity, energy metabolism flowering time. Proliferation...
Tomato chlorosis virus (ToCV) is a whitefly‐transmitted crinivirus with bipartite RNA genome inducing yellowing diseases in greenhouse and outdoor tomato plants. In this study, both genomic components of Greek isolate (Gr‐535) ToCV were sequenced. They contained 8594 nucleotides (nt) 8242 nt shared 97% 99% sequence identity Spanish American isolates, respectively. Phylogenetic analysis pairwise nucleotide amino acid comparisons showed that the clustered together isolate. Nevertheless,...
Protein phosphatase 2A catalytic subunit (PP2A-C) has a terminal leucine subjected to methylation, regulatory mechanism conserved from yeast mammals and plants. Two enzymes, LCMT1 PME1, methylate demethylate PP2A-C, respectively. The physiological importance of these posttranslational modifications is still enigmatic. We investigated processes in Arabidopsis thaliana by mutant phenotyping, global expression analysis, monitoring methylation status PP2A-C under different environmental...
Eukaryotic protein phosphatase 4 (PP4) is a PP2A-type that part of conserved complex with regulatory factors PSY2 and PP4R2. Various lines Arabidopsis thaliana mutated PP4 subunit genes were constructed to study the so far completely unknown functions in plants. Mutants knocked out putative functional homolog LIKE (PSY2L) gene dwarf bushy, while plants PP4R2 (PP4R2L) looked very similar WT. The psy2l seedlings had short roots disorganized morphology impaired meristem. Seedling growth was...
We recently developed the first algorithms specifically for plants to predict proteins carrying peroxisome targeting signals type 1 (PTS1) from genome sequences.Citation1 As validated experimentally, prediction methods are able correctly unknown peroxisomal Arabidopsis and infer novel PTS1 tripeptides. The high performance is primarily determined by large number sequence diversity of underlying positive example sequences, which mainly derived EST databases. However, a few constructs remained...
ABSTRACT Heteromeric acetyl-CoA carboxylase (ACCase) catalyzes the ATP-dependent carboxylation of to produce malonyl-CoA, committed step for de novo fatty acid synthesis. In plants, ACCase activity is controlled at multiple levels, including negative regulation by biotin attachment domain-containing (BADC) proteins, which badc1/3 double mutant leads increased seed triacylglycerol accumulation. Unexpectedly, Arabidopsis also accumulates more protein. The metabolic consequences from both...
Peroxisomes are eukaryotic specific organelles that perform diverse metabolic functions including fatty acid β-oxidation, reactive species metabolism, photorespiration, and responses to stress. However, the potential regulation of these by post-translational modifications, protein phosphorylation, has had limited study. Recently, we identified catalogued a large number peroxisomal phosphorylated proteins, implicating presence kinases in this organelle. Here, employed available prediction...
Abstract Protein phosphatase 2A (PP2A) is a heterotrimeric conserved serine/threonine complex including catalytic, scaffolding, and regulatory subunit. Three A Subunits, 17 B subunits, five C subunits are encoded by the Arabidopsis genome, allowing 255 possible PP2A holoenzyme combinations. The crucial for substrate specificity localization classified into B, B’, B’’ non-related families in land plants. In , close homologs B’η, B’θ, B’γ, B’ζ further subfamily of B’ called B’η. Previous...
Protein phosphatase 2A (PP2A) is a heterotrimeric conserved serine/threonine complex that includes catalytic, scaffolding, and regulatory subunits. The 3 A subunits, 17 B 5 C subunits are encoded by the Arabidopsis genome allow 255 possible PP2A holoenzyme combinations. crucial for substrate specificity localization classified into B, B’, B” non-related families in land plants. In Arabidopsis, close homologs B’η, B’θ, B’γ, B’ζ further subfamily of B’ called B’η. Previous studies have...
Members of the calcium-dependent protein kinase (CDPK/CPK) and SNF-related (SnRK) superfamilies are commonly found in plants some protists. Our knowledge client specificity members this superfamily is fragmentary. As family represented by over 30 Arabidopsis thaliana, identification kinase-specific overlapping relationships crucial to our understanding nuances large kinases as directed towards signal transduction pathways. Herein, we used (KiC) assay—a relative, quantitative, high-throughput...