A5012550371- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Plant Micronutrient Interactions and Effects
- Polyamine Metabolism and Applications
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Vitamin C and Antioxidants Research
- Amino Acid Enzymes and Metabolism
- Cassava research and cyanide
- Plant Gene Expression Analysis
- Cellular transport and secretion
- Plant tissue culture and regeneration
- Lipid metabolism and biosynthesis
- Plant responses to elevated CO2
- Postharvest Quality and Shelf Life Management
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Graphite, nuclear technology, radiation studies
- Endoplasmic Reticulum Stress and Disease
- Plant and Biological Electrophysiology Studies
- Allelopathy and phytotoxic interactions
- Nuclear Structure and Function
Consejo Superior de Investigaciones Científicas
2006-2024
Instituto de Biología Molecular y Celular de Plantas
2020-2024
Universitat Politècnica de València
2020-2024
Centro Nacional de Biotecnología
2006-2020
Aristotle University of Thessaloniki
2000-2020
Universidad Autónoma de Madrid
2006-2012
Instituto de la Grasa
2010
University of Crete
2008
Joint Center for Structural Genomics
2005
University of California, Riverside
2005
Humans are unable to synthesise L-ascorbic acid (L-AA, ascorbate, vitamin C), and thus entirely dependent upon dietary sources meet needs. In both plant animal metabolism, the biological functions of centred around antioxidant properties this molecule. Considerable evidence has been accruing in last two decades importance L-AA protecting not only from oxidative stress, but also mammals various chronic diseases that have their origins stress. Evidence suggests plasma levels large sections...
In contrast to animals, where polyamine (PA) catabolism efficiently converts spermine (Spm) putrescine (Put), plants have been considered possess a PA catabolic pathway producing 1,3-diaminopropane, Delta(1)-pyrroline, the corresponding aldehyde, and hydrogen peroxide but unable back-convert Spm Put. Arabidopsis (Arabidopsis thaliana) genome contains at least five putative oxidase (PAO) members with yet-unknown localization physiological role(s). AtPAO1 was recently identified as an enzyme...
The protein storage vacuole (PSV) is a plant-specific organelle that accumulates reserve proteins, one of the main agricultural products obtained from crops. Despite importance this process, cellular machinery required for transport and accumulation proteins remains largely unknown. Interfering with to PSVs has been shown result in secretion cargo. Therefore, suitable marker could be used as an assay identify mutants pathway. CLV3, negative regulator shoot stem cell proliferation,...
One of the drawbacks in improving aroma properties tomato (Solanum lycopersicum) fruit is complexity this organoleptic trait, with a great variety volatiles contributing to determine specific quality features. It well established that oxylipins hexanal and (Z)-hex-3-enal, synthesized through lipoxygenase pathway, are among most important compounds impart correct proportion some unique fresh notes tomato. Here, we confirm all enzymes responsible for synthesis these C6 present active fruit....
Summary C is ‐(+)‐12‐oxo‐phytodienoic acid ( OPDA ) likely to play signaling roles in plant defense that do not depend on its further conversion the phytohormone jasmonic acid. To elucidate role of S olanum lycopersicum (tomato) defense, we have silenced 12‐oxophytodienoate reductase 3 OPR gene. Two independent transgenic tomato lines Si 3‐1 and 3‐2 showed significantly reduced expression upon infection with necrotrophic pathogen B otrytis cinerea . Moreover, plants are more susceptible this...
Humans are unable to synthesise L-ascorbic acid (L-AA, ascorbate, vitamin C), and thus entirely dependent upon dietary sources meet needs. In both plant animal metabolism, the biological functions of centred around antioxidant properties this molecule. Considerable evidence has been accruing in last two decades importance L-AA protecting not only from oxidative stress, but also mammals various chronic diseases that have their origins stress. Evidence suggests plasma levels large sections...
Lipid droplets (LDs) are ubiquitous organelles in plant cells, but their physiological roles largely unknown. To gain insight into the function of LDs plants, we have characterized Arabidopsis homologs SEIPIN proteins, which crucial factors for LD biogenesis yeast and animals. SEIPIN1 is expressed almost exclusively embryos, while SEIPIN2 SEIPIN3 broader expression profiles with maximal levels embryos pollen, where accumulate most abundantly. Genetic analysis demonstrates that all three...
The lipoxygenase pathway is responsible for the production of oxylipins, which are important compounds plant defence responses. Jasmonic acid, final product allene oxide synthase/allene cyclase branch pathway, regulates wound-induced gene expression. In contrast, C6 aliphatic aldehydes produced via an alternative catalysed by hydroperoxide lyase, themselves toxic to pests and pathogens. Current evidence on subcellular localization conflicting, regulation metabolic channelling between two...
Ascorbate oxidase (AO) is a cell wall-localized enzyme that uses oxygen to catalyse the oxidation of ascorbate (AA) unstable radical monodehydroascorbate (MDHA) which rapidly disproportionates yield dehydroascorbate (DHA) and AA, thus contributes regulation AA redox state. Here, it reported in vivo lowering apoplast state, through increased AO expression transgenic tobacco (Nicotiana tabacum L. cv. Xanthi), exerts no effects on levels genes involved recycling under normal growth conditions,...
Protein phosphorylation is a key molecular switch used to transmit information in biological signalling networks. The output of these circuits governed by the counteracting activities protein kinases and phosphatases that determine direction switch. Whereas many have been functionally characterized, it has difficult ascribe precise cellular roles plant phosphatases, which are encoded enlarged gene families may provide high degree genetic redundancy. In this work we analysed role planta...
In angiosperms, wound-derived signals travel through the vasculature to systemically activate defence responses throughout plant. Arabidopsis thaliana, activity of vasculature-specific Clade 3 glutamate receptor-like (GLR) channels is required for transmission electrical and cytosolic Ca
Significance Vacuoles play crucial roles in plant growth and adaptation to the environment. However, mechanisms responsible for transporting membranes contents vacuoles remain largely uncharacterized, pathways compartments involved are not fully charted. We report on characterization of 17 vacuolar trafficking mutants that define a set 13 cellular factors transport soluble proteins Arabidopsis thaliana . show two these factors, GARP tethering complex subunits VPS51 VPS54, reside an ER-...
Summary The plant cell wall constitutes an essential protection barrier against pathogen attack. In addition, cell‐wall disruption leads to accumulation of jasmonates ( JA s), which are key signaling molecules for activation inducible defense responses. However, whether s in return modulate the composition reinforce this defensive remains unknown. enzyme 13–allene oxide synthase (13– AOS ) catalyzes first committed step towards biosynthesis s. potato S olanum tuberosum ), there two putative...
The transcriptional regulator MINIYO (IYO) is essential and rate-limiting for initiating cell differentiation in Arabidopsis thaliana. Moreover, IYO moves from the cytosol into nucleus cells at meristem periphery, possibly triggering their differentiation. However, genetic mechanisms controlling nuclear accumulation were unknown, evidence that increased levels trigger remained correlative. Searching interactors, we identified RPAP2 Mate (RIMA), a homolog of yeast human proteins linked to...
The transition of stem cells from self-renewal into differentiation is tightly regulated to assure proper development the organism. Arabidopsis MINIYO (IYO) and its mammalian orthologue RNA Polymerase II Associated Protein 1 (RPAP1) are essential factors for initiating cell in plants animals. Moreover, there evidence suggesting that translocation IYO RPAP1 cytosol nucleus functions as a molecular switch initiate this fate transition. Identifying determinants subcellular localization would...
Inhibiting transcriptional elongation is a recurrent mechanism to keep cells in an undifferentiated, pluripotent state metazoans. It remains, however, unclear whether lifting the barrier acts as switch initiate differentiation those organisms. Recent results suggest that such for turning on does exist plants. We argue targeting phase of transcription may be solution adopted widely evolution allow global changes needed cellular differentiation.