A5084653285- Viral Infectious Diseases and Gene Expression in Insects
- Microfluidic and Bio-sensing Technologies
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Membrane Separation Technologies
Boehringer Ingelheim (United States)
2017-2018
Tangential flow filtration (TFF) and alternating tangential (ATF) technologies using hollow fiber membranes are commonly utilized in perfusion cell culture for the production of monoclonal antibodies; however, product retention remains a known common problem with these systems. To address this issue, commercially available fibers ranging from several hundred kilo-Daltons (kDa) to 0.65 μm nominal pore size tested all demonstrated undergo moderate severe retention. Further investigation...
Perfusion processes typically require removal of a continuous or semi-continuous volume cell culture in order to maintain desired target density. For fast growing lines, the product loss from this stream can be upwards 35%, significantly reducing overall process yield. As removed is directly proportional growth, ability modulate growth during perfusion production thus becomes crucial. Leveraging existing media components achieve such control without introducing additional supplements most...