A5101777720- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Barrier Structure and Function Studies
- Acute Ischemic Stroke Management
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Atherosclerosis and Cardiovascular Diseases
- Immune Response and Inflammation
- Traumatic Brain Injury and Neurovascular Disturbances
- Nitric Oxide and Endothelin Effects
- Extracellular vesicles in disease
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neuropharmacology Research
- Intracranial Aneurysms: Treatment and Complications
- Genomics, phytochemicals, and oxidative stress
- Cerebrovascular and Carotid Artery Diseases
- Ferroptosis and cancer prognosis
- Gut microbiota and health
- Intracerebral and Subarachnoid Hemorrhage Research
- Alkaloids: synthesis and pharmacology
- Thermal Regulation in Medicine
- Tryptophan and brain disorders
- Cerebrovascular and genetic disorders
The University of Texas Health Science Center at Houston
2022-2025
Kingmed Diagnostics
2025
Louisiana State University Health Sciences Center Shreveport
2014-2024
Texas Medical Center
2024
West Virginia University
2014-2021
Blanchette Rockefeller Neurosciences Institute
2018-2020
Neurosciences Institute
2018
West Virginia University Hospitals
2015
Portland VA Medical Center
2010-2011
Oregon Health & Science University
2011
Evaluation of infarct volumes and infiltrating immune cell populations in mice after middle cerebral artery occlusion (MCAO) strongly implicates a mixture both pathogenic regulatory subsets stroke pathogenesis recovery. Our goal was to evaluate the contribution B cells development MCAO by comparing functional outcomes wild-type (WT) versus B-cell-deficient μMT<sup>−/−</sup> mice. The results clearly demonstrate larger volumes, higher mortality, more severe deficits, increased numbers...
Background and Purpose— The blood–brain barrier (BBB) is a selectively permeable cerebrovascular endothelial that maintains homeostasis between the periphery central nervous system. BBB disruption consequence of ischemic stroke permeability can be altered by infection/inflammation, but complex cellular molecular changes result in this alteration need to elucidated determine mechanisms. Methods— Infection mimic (lipopolysaccharide) challenge on infarct volume, permeability, infiltrated...
Tumor necrosis factor alpha (TNF-α) is known to exacerbate ischemic brain injury; however, the mechanism unknown. Previous studies have evaluated effects of TNF-α on neurons with long exposures high doses TNF-α, which not pathophysiologically relevant. We characterized rapid basal respiration, ATP production, and maximal respiration using relevant, post-stroke concentrations TNF-α. observed a reduction in mitochondrial function as early 1.5 h after exposure low followed by decrease cell...
The blood–brain barrier is composed of cerebrovascular endothelial cells and tight junctions, maintaining its integrity crucial for the homeostasis neuronal environment. Recently, we discovered that mitochondria play a critical role in integrity. We report first time novel mechanism underlying integrity: miR-34a mediated regulation through mitochondrial mechanism. Bioinformatics analysis suggests targets several mitochondria-associated gene candidates. demonstrated triggers breakdown cell...
Evaluation of infarct volumes and infiltrating immune cell populations in mice after middle cerebral artery occlusion strongly implicates a mixture both pathogenic regulatory subsets that affect stroke outcome. Our goal was to evaluate the contribution well-described coinhibitory pathway, programmed death (PD)-1, development occlusion.Infarct volumes, functional outcomes, effects on were compared wild-type C57BL/6 versus PD-1-deficient 60 minutes 96 hours reperfusion.The results clearly...
Oxidative stress is linked to mitochondrial dysfunction in aging and neurodegenerative conditions. The transcription factor nuclear E2–related 2 (Nrf2)–antioxidant response element (ARE) regulates intracellular antioxidative capacity combat oxidative stress. We examined the effect of <i>tert</i>-butylhydroquinone (tBHQ), an Nrf2-ARE signaling pathway inducer, on function during challenge neurons. tBHQ prevented glutamate-induced cytotoxicity HT-22 neuronal cell line even with 8-hour exposure...
Abstract Acute stroke causes complex, pathological, and systemic responses that have not been treatable by any single medication. In this study, using a murine transient middle cerebral artery occlusion model, novel therapeutic strategy is proposed, where blood replacement (BR) robustly reduces infarctions improves neurological deficits in mice. Our analyses of immune cell subsets suggest BR therapy substantially decreases neutrophils following stroke. Electrochemiluminescence detection...
A third of ischemic stroke cases have no traditional underlying causes such as hypertension, diabetes, atherosclerosis, obesity, or age. Moreover, thirty to forty percent strokes occur during acutely after an active infection and the incidence increases flu season. We others shown that combination a minor bacterial mimic, 100 μg/kg lipopolysaccharide (LPS) prior stroke—30 min transient middle cerebral artery occlusion (tMCAO)—exacerbates infarct volume in mouse model. Thus, experimental...
It is generally accepted that gestational xenobiotic exposures result in systemic consequences the adult F1 generation. However, data on detailed behavioral and cognitive remain limited. Using our whole-body nanoparticle inhalation facility, pregnant Sprague-Dawley rats (gestational day [GD] 7) were exposed 4 d/wk to either filtered air (control) or nano-titanium dioxide aerosols (nano-TiO2; count median aerodynamic diameter of 170.9 ± 6.4 nm, 10.4 0.4 mg/m3, 5 h/d) for 7.8 0.5 d remaining...
Abstract Blood-brain barrier (BBB) dysfunction occurs in cerebrovascular diseases and neurodegenerative disorders such as stroke. Opening of the BBB during a stroke has negative impact on acute outcomes. We have recently demonstrated that miR-34a regulates by targeting cytochrome c (CYC) vitro . To investigate role stroke, we purified primary endothelial cells (pCECs) from mouse brains following 1 h transient middle cerebral artery occlusion (tMCAO) measured real-time PCR to detect levels....
Aneurysmal subarachnoid hemorrhage (aSAH) causes systemic changes that contribute to delayed cerebral ischemia (DCI) and morbidity. Circulating metabolites reflecting underlying pathophysiological mechanisms warrant investigation as biomarker candidates. Blood samples, prospectively collected within 24 hours (T1) of admission 7-days (T2) post ictus, from patients with acute aSAH two tertiary care centers were retrospectively analyzed. Samples healthy subjects non-neurologic critical illness...
Rationale: Mass vaccination, low cost of immunoglobulins, and new drugs led to the emergence new, unusual patterns hepatitis B serum markers. This study reported a rare case with all 5 positive markers, including HBsAg, HBsAb, HBeAg, HBeAb, HBcAb. Patient concerns: A 30-year-old female patient was admitted due abnormal liver function. The markers were (+), After antiviral therapy, she discharged normal function decreased virus-DNA levels, but still positive. Regular follow-up conducted every...
INTRODUCTION: Delayed cerebral ischemia (DCI) is a devastating complication from aneurysmal subarachnoid hemorrhage (aSAH) without universally-accepted prediction model. miR34c belongs to micro-RNA family that has shown play pivotal roles in neurologic diseases, including neuroinflammatory disorders, drug-resistant epilepsy, and reperfusion injury ischemic stroke, but its association with DCI not been studied. METHODS: This prospective study based on aSAH patients admitted the neuroscience...
Abstract Introduction Stroke‐associated pneumonia (SAP) is a major cause of mortality in patients who have suffered from severe ischemic stroke. Although multifactorial nature, stroke‐induced immunosuppression plays key role the development SAP. Previous studies using murine model transient middle cerebral artery occlusion (tMCAO) shown that focal stroke induction results functional defects lymphocytes spleen, thymus, and peripheral blood, leading to spontaneous bacterial infection lungs...
Stroke is the second leading cause of death worldwide. The prognostic influence body temperature on acute stroke in patients has been recently reported; however, hypothermia confounded experimental results animal models. This work aimed to investigate how could prognose severity as well reveal a possible mitochondrial mechanism association and severity. Lipopolysaccharide (LPS) compromises oxidative phosphorylation cerebrovascular endothelial cells (CVECs) worsens murine stroke. In this...