Exosomes derived from lung cancer cells confer cisplatin (DDP) resistance to other cells. However, the underlying mechanism is still unknown. A549 DDP (A549/DDP) was established. Microarray used analyze microRNA (miRNA) expression profiles of cells, A549/DDP exosomes, and exosomes. There a strong correlation miRNA between exosomes their maternal A total 11 miRNAs were significantly upregulated both in compared with contrast 31 downregulated also observed. miR-100-5p most prominent decreased...

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death. This study aimed to examine roles DHRS4-AS1/miR-224-3p signaling in stemness NSCLC. Real-time PCR showed that DHRS4-AS1 was downregulated cancerous tissues, and bioinformatics analysis revealed high expression indicated a good prognosis for NSCLC patients. Sphere colony formation assays overexpression significantly suppressed stem cell-like properties. also abrogated OCT4, SOX2, CD34, CD133, markedly inhibited...

Abstract Most of the patients with lung cancer are diagnosed at advanced stage, and they often lose opportunity surgical therapy, most whom fail to reach good prognosis after chemotherapy. Recently, a few clinical studies have confirmed role adoptive T-cell transfer in maintenance therapy patients. Here, we provided statistical insights into CIKs from three different levels, cell model (in vitro co-culture system), mice situ cancer), research progression stages). We optimized components...

Abstract Background Rupture of atherosclerotic plaque can cause acute malignant heart and cerebrovascular events, such as coronary disease, stroke so on, which seriously threaten the safety human life property. Therefore, early diagnosis inhibition progress still be a vital task. Results In this study, we presented development composite mesoporous silica nanoparticle (Ru(bpy) 3 @SiO 2 -mSiO , CMSN)-based nanomedicines (NMs) @SRT1720@AntiCD36, CMSN@SRT@Anti) for accurate treatment...

Abstract To gain an insight into the molecular mechanisms of cetuximab resistance in colorectal cancer, we generated a cetuximab‐resistant cell line (H508/ CR ) and performed RNA sequencing to identify differential expression patterns noncoding s (nc s) mRNA between cetuximab‐sensitive resistant cells. A total 278 nc transcripts 1,059 were dysregulated The levels nine selected long (lnc validated using quantitative real‐time PCR . Functional analysis revealed that several groups lnc might be...

Object: To identify genes of prognostic value which associated with tumor microenvironment (TME) in acute myeloid leukemia (AML). Methods and materials: Level 3 AML patients gene transcriptome profiles were downloaded from The Cancer Genome Atlas (TCGA) database. Clinical characteristics survival data extracted the Genomic Data Commons (GDC) tool. Then, limma package was utilized for normalization processing. ESTIMATE algorithm used calculating immune, stromal scores. We examined...

Lung cancer is one of the most fatal malignancies and leading cause death worldwide. β-Elemene, a well-known anticancer drug, has drawn great deal attention from researchers attributed to its limited side impacts. N6-Methyladenosine (m6A) modification common RNA plays vital role in pathogenesis multiple tumors. However, functional link between β-elemene m6A lung development remains unexplored. In this study, we investigated whether was responsible for impacts on cancer. Firstly, outcomes...

This study aims to evaluate the influence of rumen-protected methionine (RPM) on milk yield and compositions dairy cows by employing a meta-analysis method. The articles in publication databases between January 2010 2022 which reported various concentrations RPM supplements cow diets then monitored were searched. A total 14 studies included, covering 27 treatments with 623 cows. Comprehensive Meta-Analysis V3 was used for statistical analysis, forest map drawn standard mean difference (SMD)...

Objective: This study aimed to explore the clinical efficacy and safety of durvalumab combined with albumin-bound paclitaxel carboplatin as neoadjuvant therapy for resectable stage III Non-small Cell Lung Cancer (NSCLC). Methods: A single-arm open-label phase Ib was conducted. total 40 patients driver gene-negative NSCLC were enrolled. All received treatment in combination carboplatin. The efficacy, Major Pathological Response (MPR), Complete (pCR), assessed. Flow cytometry used detect...

Abstract Background Diagnostic methods for urothelial cancer (UC) are often invasive, while urinary cytology, a non-invasive alternative, suffers from limited sensitivity. This study aimed to identify differentially methylated markers in tumor DNA and develop diagnostic method enhance the sensitivity of UC detection. Methods Whole-genome bisulfite sequencing deep methylation were employed significantly hypermethylated UC-associated genes clinical samples public datasets. Further screening...

The objective of this study is to evaluate the predictive value quantitative parameters from spectral CT for Ki-67 expression in ovarian cancer (OC). Spectral imaging data 39 patients with by pathology, encompassing 52 lesions overall, were collected retrospectively and split into two groups based on immunohistochemical results. Tumor solid components arterial, venous, delayed phases can be measured using post-processing software obtain CT. An independent sample t-test was implemented...

Endogenous bornavirus-like nucleoprotein elements (EBLNs) have been discovered in the genomes of various animals including humans, whose functions seldom studied. To explore biological human EBLNs, we constructed a lentiviral vector expressing short-hairpin RNA against EBLN1, which successfully inhibited EBLN1 expression by above 80% infected oligodendroglia cells (OL cells). We found that silencing suppressed cell proliferation, induced G2/M phase arrest, and promoted apoptosis OL cells....

Colorectal cancer (CRC) is the fifth leading cause of cancer-related death in China. The incidence Chinese CRC has increased dramatically with changes dietary and lifestyle. However, genetic landscape colorectal mutation still poorly understood. In this study, we have performed whole exome-sequencing analysis 63 cases. We found that were hypermutated, which enriched ECM-receptor interaction, antigen processing presentation, focal adhesion. Analysis clinical characteristics indicated...

Aim: This study was designed to investigate the predictive and prognostic values of serum vascular endothelial growth factor (VEGF) level in non-small cell lung cancer (NSCLC) patients treated with platinum-based chemotherapy. Methods: Patients' peripheral blood samples were collected prior chemotherapy after 1 week third cycle combination Serum VEGF levels evaluated through Luminex multiplex technique. Between September 2011 August 2015, a total 135 consecutive advanced or recurrent...

Non-small cell lung cancer (NSCLC) is one of the leading causes cancer-related deaths, and it also most frequently diagnosed cancer. Previous studies indicate that IL-33 plays a crucial role in development NSCLC. In recent years, miRNAs has become increasingly clear. However, reports focused on relation between NSCLC have been limited.The expression miR-128-3p was detected by qPCR. MTT, EdU, colony formation assays were used to detect proliferation ability cells. Transwell assay investigate...

Chemotherapy-induced cognitive impairment (CICI), termed "chemobrain", is highly prevalent in cancer patients following the administration of chemotherapeutic agents. However, potential pathophysiological mechanisms underlying CICI remain unknown. This study aimed to explore functional changes brain and associated non-small cell lung (NSCLC) receiving different chemotherapy regimen.A total 49 NSCLC (25 pemetrexed plus carboplatin (PeCC) 24 paclitaxel (PaCC)) 61 healthy controls (HCs) were...

To understand the mechanism involved in gefitinib resistance, we established gefitinib-resistant human HCC827/GR-8-1 cell line from parental HCC827 line. We compared micro-RNA expression profiles of cells using Agilent microarrays. The micro-RNAs, such as miR-149-5p, were up- or downregulated and associated with acquired resistance. Quantitative real-time polymerase chain reaction was then performed to verify patterns different micro-RNAs. result showed that miR-149-5p upregulated...

Emotional distress frequently occur in cancer patients following diagnosis. Previous neuroimaging studies have demonstrated that depression and anxiety are associated with functional structural brain abnormalities. However, little is known about the cancer-associated changes of emotional network non-small cell lung (NSCLC) patients. The aim this study was to assess topological features emotions non-nervous system metastatic NSCLC prior chemotherapy.Twenty-four treatment-naïve 25 healthy...

Cancer and chemotherapy are correlated with brain functional structural changes in cancer patients, which may lead to cognitive dysfunction. However, little is known about the abnormalities of patients non-small cell lung (NSCLC). The aim this study was explore topological properties within white matter network NSCLC prior chemotherapy.To neurobiological biomarkers NSCLC, magnetic resonance imaging (MRI) data were acquired 24 non-nervous system metastatic 25 matched healthy controls....

The recent research shows that the inhibition of nuclear factor-κB (NF-κB) pathway is a promising therapeutic option for patients who progress after treatment with novel mutant-selective EGFR-TKIs. For propose to find nontoxic drug reverse acquired gefitinib resistance, we examined whether Liver X Receptors agonist GW3965 affect resistance HCC827/GR-8-2 cells. Cell viability was measured by CCK-8 assay. Levels NF-κB, p-AKT and caspases were detected Western blot analysis. Immunocytochemical...

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) are efficient in treating patients with non-small cell lung cancer (NSCLC) harboring EGFR activating mutations. Unfortunately, nearly all ultimately acquire resistance to EGFR-TKI treatment. Liver X receptors (LXRs) can regulate tumor various lines. The present study indicated that LXR agonist combined gefitinib weakened Akt-nuclear (NF)-κB activation and inhibited the expression levels of apoptosis-related proteins...

Epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) is effective in lung cancer patients carrying sensitive EGFR mutations. In this study, we investigated if liver X receptor (LXR) agonist T0901317 could reverse the resistance of cell lines A549 and H1650 to EGFR-TKI treatment. We found that make natural EGFR-TKI-resistant human cells treatment was dependent on LXRβ expression. However, does not have a similar effect another line H1650.