A5053496877- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- interferon and immune responses
- Herpesvirus Infections and Treatments
- Plant Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Virology and Viral Diseases
- Vector-Borne Animal Diseases
- RNA Interference and Gene Delivery
- Insect and Pesticide Research
- COVID-19 Impact on Reproduction
- RNA Research and Splicing
- RNA regulation and disease
- Transgenic Plants and Applications
- Mosquito-borne diseases and control
- Carbon and Quantum Dots Applications
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Respiratory viral infections research
Huazhong Agricultural University
2016-2025
Center of Hubei Cooperative Innovation for Emissions Trading System
2016-2025
State Key Laboratory of Agricultural Microbiology
2012-2018
Sustainable Innovation (Sweden)
2015
Wuhan College
2015
Universidade Nova de Lisboa
2015
University of Lisbon
2015
Jiangsu Academy of Agricultural Sciences
2012
With the gradual usage of carbon dots (CDs) in area antiviral research, attempts have been stepped up to develop new CDs with high biocompatibility and effects. In this study, a kind highly biocompatible (Gly-CDs) is synthesized from active ingredient (glycyrrhizic acid) Chinese herbal medicine by hydrothermal method. Using porcine reproductive respiratory syndrome virus (PRRSV) as model, it found that Gly-CDs inhibit PRRSV proliferation 5 orders viral titers. Detailed investigations reveal...
The research of carbon-based antivirals is still in its infancy, and their development into safe effective carbon dots (CDs) with antiviral activity at multiple points the life cycle virus remains to be explored. Here, we report a one-step method apply curcumin order prepare uniform stable cationic (CCM-CDs) properties. inhibitory effect CCM-CDs on viral replication was studied by using porcine epidemic diarrhea (PEDV) as coronavirus model. PEDV applied model study as-prepared replication....
ABSTRACT Porcine epidemic diarrhea virus (PEDV), a porcine enteropathogenic coronavirus, causes lethal watery in piglets and results large economic losses many Asian European countries. A large-scale outbreak of occurred China 2010, the emerged United States 2013 spread rapidly, posing significant public health concerns. Previous studies have shown that PEDV infection inhibits synthesis type I interferon (IFN), viral papain-like protease 2 has been identified as an IFN antagonist. In this...
ABSTRACT The leader proteinase (L pro ) of foot-and-mouth disease virus (FMDV) is a papain-like that plays an important role in FMDV pathogenesis. Previously, it has been shown L involved the inhibition type I interferon (IFN) response by FMDV. However, underlying mechanisms remain unclear. Here we demonstrate Lb , shorter form deubiquitinating activity. Sequence alignment and structural bioinformatics analyses revealed catalytic residues (Cys51 His148) are highly conserved all seven...
ABSTRACT Porcine epidemic diarrhea virus (PEDV) is an enteropathogenic coronavirus causing lethal watery in piglets. Since 2010, a PEDV variant has spread rapidly China, and it emerged the United States 2013, posing significant economic public health concerns. The ability to circumvent interferon (IFN) antiviral response, as suggested for PEDV, promotes viral survival regulates pathogenesis of infections, but underlying mechanisms remain obscure. Here, we show that PEDV-encoded 3C-like...
Development of novel antiviral reagents is great importance for the control virus spread. Here, Ag2S nanoclusters (NCs) were proved first time to possess highly efficient activity by using porcine epidemic diarrhea (PEDV) as a model coronavirus. Analyses titers showed that NCs significantly suppressed infection PEDV about 3 orders magnitude at noncytotoxic concentration 12 h postinfection, which was further confirmed expression viral proteins. Mechanism investigations indicated treatment...
Foot-and-mouth disease is a highly contagious viral illness of wild and domestic cloven-hoofed animals. The causative agent, foot-and-mouth virus (FMDV), replicates rapidly, efficiently disseminating within the infected host being passed on to susceptible animals via direct contact or aerosol route. To survive in host, FMDV has evolved block interferon (IFN) response. Previously, we others demonstrated that leader proteinase (L(pro)) an IFN antagonist. Here, report another FMDV-encoded...
ABSTRACT Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus. The first outbreak of PDCoV was announced from the United States in 2014, followed by reports Asia. nonstructural protein nsp5 a 3C-like protease coronavirus, and our previous study showed that inhibits type I interferon (IFN) production. In this study, we found significantly inhibited IFN-stimulated response element (ISRE) promoter activity transcription genes (ISGs), suggesting also suppresses IFN...
Porcine reproductive and respiratory syndrome virus (PRRSV) transmissible gastroenteritis (TGEV) are two highly infectious lethal viruses causing major economic losses to pig production. Here, we report generation of double-gene-knockout (DKO) pigs harboring edited knockout alleles for known receptor proteins CD163 pAPN show that DKO completely resistant genotype 2 PRRSV TGEV. We found no differences in meat-production or reproductive-performance traits between wild-type pigs, but detected...
Interleukin-6 (IL-6) is a pleiotropic cytokine that plays central role in host defense. IL-6 expression can be regulated at both transcriptional and post-transcriptional level. We used combination of bioinformatics experimental techniques to demonstrate the miR-365 direct negative regulator IL-6. Overexpression mimics decreased activity luciferase reporter containing 3′-UTR led repression protein. In contrast, ectopic inhibitor elevated expression. The regulation was strictly dependent on...
Porcine epidemic diarrhea virus (PEDV), which re-emerged in China October 2010, has spread rapidly worldwide. Detailed analyses of the complete genomes different PEDV strains are essential to understand relationships among re-emerging and historic Here, we analysed 409 from countries, were classified into five subgroup (i.e., GI-a, GI-b, GII-a, GII-b, GII-c). Phylogenetic study genes revealed that newly discovered GII-c exhibited inconsistent topologies between spike gene other genes....
Developing nanomaterials-based antimicrobial agents has shown a widespread promise. In this study, silver nanoparticle-modified graphene oxide (GO-AgNPs) nanocomposites were self-assembled via interfacial electrostatic force. By using the porcine reproductive and respiratory syndrome virus (PRRSV) as pattern, antiviral effect of as-prepared GO-AgNPs on replication was investigated. The results indicated that exposure with could obviously suppress PRRSV infection. It found exhibited better...
ABSTRACT Porcine deltacoronavirus (PDCoV) has recently emerged as an enteric pathogen that can cause serious vomiting and diarrhea in suckling piglets. The first outbreak of PDCoV occurred the United States 2014 was followed by reports South Korea, China, Thailand, Lao People's Democratic Republic, Vietnam, leading to economic losses for pig farms posing a considerable threat swine industry worldwide. Our previous studies have shown encodes three accessory proteins, NS6, NS7, NS7a, but...
Porcine reproductive and respiratory syndrome virus (PRRSV) is a typical immunosuppressive devastating the global swine industry. DEAD-box helicases (DDXs) are family of ATP-dependent RNA that predominantly implicated in modulating cellular metabolism. Meanwhile, growing number studies have suggested some DDXs associated with innate immunity infection, so they considered potential antiviral targets. Herein, we screened 40 found ectopic expression DDX10 exhibited significant anti-PRRSV...
Since it was first reported in 2013, the NADC30-like PRRSV has been epidemic China. Hubei Province is known as China’s key hog-exporting region. To understand prevalence and genetic variation of PRRSV, herein, we detected analyzed 317 lung tissue samples from pigs with respiratory disease Province, demonstrated that strain second-most predominant during 2017–2018, following highly pathogenic (HP-PRRSV). Additionally, isolated a new strain, named CHN-HB-2018, which could be stably passaged...
Abstract Porcine epidemic diarrhea virus (PEDV) is a highly infectious intestinal coronavirus that poses significant threat to the pig industry because of its strong virulence and propensity for mutation recombination. PEDV classified into three major genotypes based on spike (S) gene sequence: G1 (classical), G2 (variant), S-INDEL (characterized by nucleotide insertions/deletions in S gene). Both have been frequently detected China, whereas strain has rarely reported or isolated. In this...
Em2007, a porcine reproductive and respiratory syndrome virus (PRRSV) variant with unique 68 aa deletion in Nsp2, was recently isolated China. Phylogenetic molecular evolutionary analyses indicated that Em2007 is natural recombinant between vaccine strain of PRRSV circulating virus. We also tested its pathogenicity piglets.