A5100710204- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Genomics and Phylogenetic Studies
- NF-κB Signaling Pathways
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Toxin Mechanisms and Immunotoxins
- Hip disorders and treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Orthopaedic implants and arthroplasty
- Plant Stress Responses and Tolerance
- HIV/AIDS Research and Interventions
Huazhong Agricultural University
2016-2025
Center of Hubei Cooperative Innovation for Emissions Trading System
2015-2025
Yunnan Agricultural University
2024-2025
Guizhou Provincial People's Hospital
2019-2025
Harbin Veterinary Research Institute
2021-2025
Chinese Academy of Agricultural Sciences
2021-2025
Guizhou University
2021-2025
Affiliated Hospital of Shaanxi University of Chinese Medicine
2025
Shaanxi University of Chinese Medicine
2025
Minzu University of China
2022-2024
ABSTRACT Porcine epidemic diarrhea virus (PEDV), a porcine enteropathogenic coronavirus, causes lethal watery in piglets and results large economic losses many Asian European countries. A large-scale outbreak of occurred China 2010, the emerged United States 2013 spread rapidly, posing significant public health concerns. Previous studies have shown that PEDV infection inhibits synthesis type I interferon (IFN), viral papain-like protease 2 has been identified as an IFN antagonist. In this...
ABSTRACT The leader proteinase (L pro ) of foot-and-mouth disease virus (FMDV) is a papain-like that plays an important role in FMDV pathogenesis. Previously, it has been shown L involved the inhibition type I interferon (IFN) response by FMDV. However, underlying mechanisms remain unclear. Here we demonstrate Lb , shorter form deubiquitinating activity. Sequence alignment and structural bioinformatics analyses revealed catalytic residues (Cys51 His148) are highly conserved all seven...
Foot-and-mouth disease is a highly contagious viral illness of wild and domestic cloven-hoofed animals. The causative agent, foot-and-mouth virus (FMDV), replicates rapidly, efficiently disseminating within the infected host being passed on to susceptible animals via direct contact or aerosol route. To survive in host, FMDV has evolved block interferon (IFN) response. Previously, we others demonstrated that leader proteinase (L(pro)) an IFN antagonist. Here, report another FMDV-encoded...
Autophagy and ER stress are involved in maintaining some well-orchestrated mechanisms aimed at either restoring cellular homeostasis or performing cell death. is a well-defined process which governs overall outcomes. Selective degradation of the mediated by autophagy occurs through specific type called ER-phagy, ensures protein homeostasis. Immunoblotting RT-PCR were used to evaluate expression ATG5 ATG7 chondrocyte. Western blotting, Flow cytometry,immunofluorescence staining confocal...
Interleukin-6 (IL-6) is a pleiotropic cytokine that plays central role in host defense. IL-6 expression can be regulated at both transcriptional and post-transcriptional level. We used combination of bioinformatics experimental techniques to demonstrate the miR-365 direct negative regulator IL-6. Overexpression mimics decreased activity luciferase reporter containing 3′-UTR led repression protein. In contrast, ectopic inhibitor elevated expression. The regulation was strictly dependent on...
The multigene family genes (MGFs) in the left variable region (LVR) of African swine fever virus (ASFV) genome have been reported to be involved viral replication primary porcine alveolar macrophages (PAMs) and virulence pigs. However, exact functions key MGFs LVR that regulate ASFV remain unclear. In this study, we identified MGF300-2R gene critical for PAMs by deleting different sets from highly virulent strain HLJ/18 (ASFV-WT). mutant lacking (Del2R) showed a 1-log reduction titer,...
African swine fever (ASF) is a highly contagious, often fatal viral disease caused by virus (ASFV) that imposes substantial economic burden on the global pig industry each year. When screening for regulation of replication genes in left variable region ASFV genome, we observed notable reduction following deletion MGF300-4L gene. However, role infection remains unexplored. In this study, found could effectively inhibit production proinflammatory cytokines IL-1β and TNF-α, which are regulated...
Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus that has been devastating the swine industry worldwide since late 1980s. To investigate impact of cellular microRNAs (miRNAs) on replication PRRSV, we screened 10 highly conserved miRNAs implicated in innate immunity or antiviral function identified miR-125b as inhibitor PRRSV replication. Virus titer western blot assays demonstrated reduced viral gene expression a dose-dependent manner both MARC-145 cell line...
ABSTRACT NEMO (NF-κB essential modulator) is a bridging adaptor indispensable for viral activation of interferon (IFN) antiviral response. Herein, we show that hepatitis A virus (HAV) 3C protease (3C pro ) cleaves at the Q304 residue, negating its signaling function and abrogating induction IFN-β synthesis via retinoic acid-inducible gene I/melanoma differentiation-associated protein 5 (RIG-I/MDA5) Toll-like receptor 3 (TLR3) pathways. cleavage IFN antagonism, however, were lost upon...
Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus that has been devastating the swine industry worldwide since late 1980s. Previous studies have reported PRRSV infection induced production of IL-1 β . However, cellular sensors signaling pathways involved in this process not elucidated yet. Here, we studied mechanisms responsible for response to highly pathogenic PRRSV. Upon primary porcine alveolar macrophages, both mRNA expression secretion were significantly...
Porcine epidemic diarrhea virus (PEDV) causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs. A large‐scale outbreak of PED occurred China 2010, PEDV emerging the United States 2013 spreading rapidly, posing significant economic public health concerns. In this study, LC–MS/MS coupled to iTRAQ labeling was used quantitatively identify differentially expressed cellular proteins PEDV‐infected Vero cells. We identified 49 proteins,...
Esophagogastric variceal bleeding (EGVB) is a serious complication of patients with decompensated cirrhosis and associated high mortality morbidity. Early diagnosis screening cirrhotic at risk for EGVB crucial. Currently, there lack noninvasive predictive models widely available in clinical practice.To develop nomogram based on variables radiomics to facilitate the prediction patients.A total 211 hospitalized between September 2017 December 2021 were included this retrospective study....
In this study, we investigated the function of OsAAI1 in yield and drought tolerance by constructing overexpression line OE-OsAAI1 mutant osaai1. Bioinformatics analysis showed that AAI gene-OsAAI1- belongs to HPS_like subfamily AAI_LTSS superfamily, was localized nucleus. The expression significantly induced ABA stress. (OE19) increased, gene (osaai1-1) repressed plant height, primary root length, lateral number, grain size rice. Moreover, physiological biochemical analyses osaai1 sensitive...
Mycoplasma, the smallest self-replicating organism with a minimal metabolism and little genomic redundancy, is expected to be close approximation set of genes needed sustain bacterial life. This study employs comparative evolutionary analysis twenty Mycoplasma genomes gain an improved understanding essential genes. By analyzing core genome mycoplasmas, we finally revealed conserved for mycoplasma survival. Further showed that has many characteristics in common experimentally identified...
Ubiquitination and deubiquitination have emerged as critical regulatory processes in the virus-triggered type I interferon (IFN) induction pathway. In this study, we carried out a targeted siRNA screen of 54 ubiquitin-specific proteases (USPs) identified USP25 negative regulator IFN signaling Overexpression inhibited virus-induced activation IFN-β, regulation factor 3 (IRF3) nuclear factor-kappa B (NF-κB), well phosphorylation IRF3 NF-κB subunit p65. Furthermore, Knockdown potentiated IFN-β....
Abstract Background Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic swine. Despite presenting with low direct mortality, EP responsible for major economic losses in pig industry. To identify virulence-associated determinants M. , we determined whole genome sequence strain 168 and its attenuated high-passage 168-L carried out comparative genomic analyses. Results We performed first comprehensive analysis hyopneumonia e made preliminary...
Viral infection triggers a series of signaling cascades, which converge to activate the transcription factors nuclear factor-κB (NF-κB) and interferon regulatory factor 3 (IRF3), thereby inducing type I interferons (IFNs). Although not fully characterized, these innate antiviral responses are fine-tuned by dynamic ubiquitination deubiquitination processes. In this study, we report ubiquitin-specific protease (USP) 15 is involved in regulation retinoic acid-inducible gene (RIG-I)-dependent...