A5022190748- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Pneumonia and Respiratory Infections
- Aquaculture disease management and microbiota
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Streptococcal Infections and Treatments
- Viral Infections and Immunology Research
- Rabies epidemiology and control
- Virology and Viral Diseases
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Blood groups and transfusion
- Genomics and Phylogenetic Studies
- Toxoplasma gondii Research Studies
- Immunotherapy and Immune Responses
- Vector-Borne Animal Diseases
- Inhalation and Respiratory Drug Delivery
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Viral Infections and Vectors
- Perovskite Materials and Applications
- Biosensors and Analytical Detection
- Reproductive tract infections research
Jiangsu Academy of Agricultural Sciences
2016-2025
Ministry of Agriculture and Rural Affairs
2020-2025
Nanjing Agricultural University
2021-2025
ARC Centre of Excellence in Advanced Molecular Imaging
2024
UNSW Sydney
2024
Nanjing Normal University
2024
University of KwaZulu-Natal
2022
Jiangsu University
2021-2022
Tibet University
2022
Ministry of Agriculture
2016
Mycoplasma, the smallest self-replicating organism with a minimal metabolism and little genomic redundancy, is expected to be close approximation set of genes needed sustain bacterial life. This study employs comparative evolutionary analysis twenty Mycoplasma genomes gain an improved understanding essential genes. By analyzing core genome mycoplasmas, we finally revealed conserved for mycoplasma survival. Further showed that has many characteristics in common experimentally identified...
Abstract Background Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic swine. Despite presenting with low direct mortality, EP responsible for major economic losses in pig industry. To identify virulence-associated determinants M. , we determined whole genome sequence strain 168 and its attenuated high-passage 168-L carried out comparative genomic analyses. Results We performed first comprehensive analysis hyopneumonia e made preliminary...
Mycoplasma hyopneumoniae is a colonizing respiratory pathogen that can cause great economic losses to the pig industry worldwide. Although putative virulence factors have been reported, pathogenesis of this species remains unclear. Here, we used virulent M. strain 168 infect swine tracheal epithelial cells (STEC) identify infection-associated by two-dimensional electrophoresis (2-DE). Whole proteins were obtained and compared with samples cultured in broth. Six differentially expressed an...
Air-liquid interface (Ali) systems allow the establishment of a culture environment more representative that in vivo than other systems. They are useful for performing mechanistic studies respiratory epithelial cells as drug permeation barriers and can be used to study interactions between hosts pathogens. However, there have been few concerning Ali cultures primary swine tracheal (STECs) an immortalized STEC line, differences these two remain poorly defined. In this study, we established...
Accurate, ultrasensitive, and point-of-care (POC) diagnosis of the African swine fever virus (ASFV) remains imperative to prevent its spread limit losses incurred. Herein, we propose a CRISPR-Cas12a-assisted triplex amplified colorimetric assay for ASFV DNA detection with ultrahigh sensitivity specificity. The specific recognition recombinase aided amplification (RAA)-amplified could activate Cas12a/crRNA/ASFV complex, leading digestion linker (bio-L1) on magnetic beads (MBs), thereby...
Abstract Mycoplasma hyorhinis infects pigs causing polyserositis and polyarthritis, has also been reported in a variety of human tumor tissues. The occurrence disease is often linked with the systemic invasion pathogen. Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), one key enzymes glycolysis, was as surface multifunctional molecule several bacteria. Here, we investigated whether GAPDH could manifest binary functions; an adhesin to promote colonization well plasminogen receptor...
ABSTRACT Mycoplasma hyopneumoniae strain 168, a pathogenic prevalent in China, was isolated 1974. Although this has been widespread for long time, the genome sequence had not determined. Here, we announce complete of M. 168.
Mycoplasmas persist in the host for a long time, suggesting that they possess mechanisms immune evasion. Factor H is negative regulator of complement system, which binds to cells avoid unexpected activation. In this study, we revealed many mycoplasmas, such as Mycoplasma hyopneumoniae, hyorhinis, hyosynoviae, gallisepticum, pneumoniae, genitalium, flocculare, and bovis could hijack factor present themselves tissue thus escape from attack. Furthermore, mechanism recruiting was identified M....
Mycoplasma hyorhinis may cause systemic inflammation of pigs, typically polyserositis and arthritis, is also associated with several types human cancer. However, the pathogenesis M. colonizing breaching respiratory barrier to establish infection poorly understood. Glycolytic enzymes are important moonlighting proteins virulence-related factors in various bacteria. In this study, we investigated functions a glycolytic critical enzyme, enolase spread hyorhinis. Bacterial surface localization...
The demonstrated link between the emergence of H3N2 variant (H3N2v) influenza A viruses (IAVs) and swine exposure at agricultural fairs has raised concerns about human health risk posed by IAV-infected swine. Understanding antigenic profiles IAVs circulating in pigs is critical to developing effective prevention control strategies. Here, 68 IAV isolates recovered from Ohio (2009 2011) were antigenically characterized. These compared with other H3 commercial swine, wild birds, canines, along...
Mycoplasma hyopneumoniae is an important respiratory pathogen that causes great economic losses to the pig industry worldwide. Although some putative virulence factors have been reported, pathogenesis remains poorly understood. Herein, we evaluated relative abundance of proteins in virulent 168 (F107) and attenuated 168L (F380) M. strains identify virulence-associated by two-dimensional electrophoresis (2-DE). Seven were found be ≥ 1.5-fold more abundant 168, protein–protein interaction...
To determine whether, and to what extent, influenza A subtype H3 viruses were present in feral swine the United States, we conducted serologic virologic surveillance during October 2011-September 2012. These animals periodically exposed infected with A(H3N2) viruses, suggesting they may threaten human animal health.
Mycoplasma hyorhinis is a common pathogen of swine and also associated with various human tumors. It causes systemic inflammation, typically polyserositis polyarthritis, in some infected pigs. However, the pathogenic mechanism M. remains unclear. DnaK highly conserved protein belonging to heat-shock 70 family molecular chaperones, which plays important roles as moonlighting bacteria. In present study, we identified surface exposure DnaK. Two virulent strains expressed more on their than...
Cumulative evidence suggests that bacterial pneumonia may contribute to the dysbiosis of gut microbiota and other gastrointestinal symptoms. Our experiment has demonstrated lung inflammation induced by M. hyorhinis infection was associated with intestinal barrier dysfunction, which provide a theoretical basis for exploring gut-lung axis based on infection.
Abstract Further improvements in photocurrent are essential to unlock higher efficiencies inverted (p‐i‐n) perovskite solar cells (PSCs). While the use of textured substrates has proven successful normal structure (n‐i‐p) devices improve photocurrent, applying same approach p‐i‐n architecture is challenging due difficulties depositing ultra‐thin self‐assembled monolayers (SAMs) on uneven surfaces. To overcome this limitation, a rubidium‐based ammonia treatment for nickel oxide seed layers...
The increasing antibiotic resistance of mycoplasma underscores the need for new therapeutic strategies, particularly those targeting alveolar macrophages (AMs). AMs are crucial pulmonary immunity but suffer from impaired immune and endocytic functions during infection due to TNF-α-induced necroptosis. This study demonstrates that icariin (ICA), a bioactive flavonoid with anti-inflammatory properties, reduces necroptosis TNF-α secretion in infected AMs, mitigating lung inflammation. However,...
Abstract Swine mycoplasma pneumonia caused by Mycoplasma hyopneumoniae is the most prevalent and frequently occurring chronic respiratory disease in pigs worldwide. Pig breeds China are more susceptible to , understanding reasons for their susceptibility crucial prevention control of swine pneumonia, as well promoting healthy development industry. has limited biosynthesis metabolism, harsh requirements nutrients, thus it necessary add approximately 15%-20% porcine serum medium during vitro...