A5064344271- PARP inhibition in cancer therapy
- Sirtuins and Resveratrol in Medicine
- Protease and Inhibitor Mechanisms
- Mitochondrial Function and Pathology
- Biochemical effects in animals
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical Acid Research Studies
- Adipose Tissue and Metabolism
- Calcium signaling and nucleotide metabolism
- RNA regulation and disease
- Adipokines, Inflammation, and Metabolic Diseases
- Autophagy in Disease and Therapy
- Blood properties and coagulation
- Neuroscience and Neuropharmacology Research
- interferon and immune responses
- Periodontal Regeneration and Treatments
- Phytochemicals and Medicinal Plants
- Immune cells in cancer
- Tendon Structure and Treatment
- Luminescence and Fluorescent Materials
- Mesenchymal stem cell research
- Diabetes Treatment and Management
- Metabolism and Genetic Disorders
- Carbon and Quantum Dots Applications
- Vitamin C and Antioxidants Research
Karolinska Institutet
2024
Palladin Institute of Biochemistry
2012-2023
National Academy of Sciences of Ukraine
2016-2023
Institute of Physics
2017
Luminescent organic dots (O-dots) were synthesized via a one-pot, solvent-free thermolysis of citric acid in urea melt. The influence the ratio precursors and duration process on properties O-dots was established mechanism their formation hypothesized. multicolour luminescence tunability toxicity extensively studied. possible applications for alive/fixed cell staining labelling layer-by-layer polyelectrolyte microcapsules evaluated.
Beneficial effects of nicotinamide (NAm) and its derivates have been earlier shown in animal models diabetes mellitus (DM), but the mechanisms their neuroprotective activities are still largely unknown. The aim present study was to investigate if NAm conjugate nicotinic acid with gamma-aminobutyric (N-GABA) able modulate expression levels apoptosis regulators, angiogenesis-related molecules, specific cytoskeletal proteins diabetic rat brain.After six weeks streptozotocin induced type 1 DM,...
Abstract Objectives. The present study was designed to assess whether apoptosis-related genes as parp-1 and bax could be targets for treatment of diabetes mellitus vitamin D may exert beneficial effects. Methods. Vitamin 3 4 weeks, starting after weeks the duration. expression estimated on mRNA levels using real time quantitative polymerase chain reaction. Results. After 8 diabetic rats had weight loss, while blood glucose increased about 4.9-fold compared control group. administration...
The molecular pathogenesis of diabetic encephalopathy (De), one the serious complications diabetes mellitus, is complex.In this study, we examined whether expression levels SIrT1 and SIrT2 were key for development brain dysfunctions PARP-1 inhibitors could affect these proteins prevention De in rats with type 1 diabetes.after 10 weeks streptozotocin-induced mellitus (70 mg/kg), Wistar male treated by i.p. injection ParP-1 inhibitors, 1.5-isoquinolinediol (ISo) or nicotinamide (Nam) (3 100...
Summary. Pericellular plasmin generation triggers apoptosis/anoikis in normal adherent cells. However, cancer cells are notoriously resistant to anoikis, enabling metastasis and new tumor growth beyond their original environment. Autophagy can be a major contributor anoikis resistance cancer. Aim: To investigate if protective autophagy induced lung adenocarcinoma response plasminogen treatment. Materials Methods: Human A549 were incubated with Glu-plasminogen (0.1–1.0 µM) for 24 h. activity...
Fibrosis of the injured muscles is a problem recovery from trauma and denervation. The aim work was to investigate interconnection matrix metalloproteinase-9 (ММР-9) activity in denervated with fibrosis estimate its role nerve restoration by epineurial suture, fibrin-based glue, polyethylene glycol hydrogel. metalloproteinases estimated gelatin zymography. Collagen density determined histochemically. An increased level active MMP-9 associated fibrous changes skeletal after an suture. use...
Plasminogen/plasmin system is known for its ability to support hemostatic balance of blood.however, plasminogen may be considered as an adhesive ligand and in this way could affect the functioning blood cells.We showed that exogenous Lys-plasminogen, but not Glu-form, inhibited platelet aggregation suppressed α-granule secretion.The aim work was investigate influence Glu-and Lysform on formation procoagulant surface using phosphatidylserine exposure a marker.human platelets were obtained...
Connective tissue is highly susceptible to imbalances induced by diabetes. Diabetes-related osteopenia, decreased bone strength etc. may be associated with altered metabolism of various collagens: Although it assumed that alterations in collagen amino acids (AA) strongly affect protein properties andphysiological functions, however, very limited evidences are present at the moment regarding AA composition type I and its relevance abnormal availability vitamins which necessary for synthesis...
The effect of nicotinamide, acetyl-L-carnitine and α-lipoic acid on the separate links carbohydrate metabolism in brain, heart liver rats for type 2 diabetes it was investigated. Combined administration caused partial reduction hyperglycemia rats. By action investigated compounds activity hexokinase glucokinase, which were reduced under by 62 84 %, respectively, as compared to control partially normalized. NAD contents diabetic decreased 46, 52 36 but elevated compounds, especially liver. It...
Abstract Ras-GTPase-activating protein SH3-domain-binding proteins (G3BP) are multifunctional RNA-binding proteins, pivotal in the initiation of stress granules (SGs). SARS-CoV-2 nucleocapsid (N) exhibits strong binding affinity for G3BP and inhibition SG formation. However, pro-viral role(s) G3BP-N interaction have remained unclear. Here, we comprehensively examined importance infection both vitro vivo. Using reverse genetics, constructed a viral mutant, RATA, which stronger more persistent...
Abstract Ras-GTPase-activating protein SH3-domain-binding proteins (G3BP) are critical for the formation of stress granules (SGs) through their RNA- and ribosome-binding properties. SARS-CoV-2 nucleocapsid (N) exhibits strong binding affinity G3BP inhibits infection-induced SG soon after infection. To study impact G3BP-N interaction on viral replication pathogenesis in detail, we generated a mutant (RATA) that specifically lacks G3BP-binding motif N protein. RATA triggers stronger more...
Aim.To investigate reactive changes of GFAP-positive astrocytes as a marker brain response following hemorrhagic stroke and HSV-I.Methods.The experiments were performed on 110 Balb/c mice weighing 18-20 g.The animals infected with HSV type I, 30 day was simulated in determined by immunohistochemistry. Gliosis confirmed density arborization astrocytes.Results.The results immunohistochemical studies increased the hippocampus I well sharp increase hypertrophy simulation animals.Conclusions.The...
Introduction. The theme of metal hypersensitivity (MHS) reactions is among the most controversial and complex issues in orthopedic trauma practice. MHS diagnoses are diagnostically challenging because they exclusionary diagnoses. It currently uncertain which biomarkers can reliably predict a potential pathological response to implants. aim this research investigate implants containing nickel (Ni). Materials methods. This was conducted on female Wistar rats accordance with standards...
Objective. The study was performed to elucidate whether nicotinamide (NAm) can attenuate the diabetes-induced liver damage by correction of ammonia detoxifying function and disbalance NAD-dependent processes in diabetic rats. Methods. After four weeks streptozotocin-induced diabetes, Wistar male rats were treated for two with or without NAm. Urea concentration, arginase, glutamine synthetase activities, NAD+ levels, NAD+/NADH ratio measured cytosolic extracts. Expression parp-1 gene...