Currently, probiotics are increasingly used as the alternative to antibiotics well preventive measures in humans. In particular, occupy a key position treatment of antibiotics-associated intestinal dysbiosis. A spore-forming microorganism lactobacillus Bacillus coagulans is one most promising probiotics. However, some its pharmacological effects remain poorly understood. This study was aimed at investigation effect B. (Laktovit Forte) on dysbiosis syndrome mice caused by streptomycin against...

The antiproliferative activity of aconitylated (AIFN) and succinylated (SIFN) derivatives recombinant interferon- alpha2b (IFN-alpha2b) was examined. Acylation IFN-alpha2b performed by succinic cis-aconitic anhydrides. Antiproliferative properties AIFN SIFN were studied in vitro on the CaOv cell line, highly sensitive to IFN, SW-480 with low sensitivity IFN-alpha2b. one lysine molecule or anhydride resulted a 3-3.5-fold increase its cells. highest acylated cells observed for both AIFNs SIFNs...

Nisin is a typical representative of class I bacteriocins belonging to subtype A1 lantibiotics. This lantibiotic produced by gram-positive bacteria the genera Lactococcus, Streptococcus, Staphylococcus, and Blautia. Currently, at least twelve natural variants nisin are known. They differ in some physicochemical properties biological activity. generally recognized as safe (GRAS) has been used food additive for seven decades. The current review presents data from scientific reports over past...

Purpose: The biological activity (direct anticancer action in vitro) of combinatorial IL-2 succinylated derivatives was examined. Acylation performed using succinic anhydride with various levels acylation, the formation a complex assembly many (self-assembled quasi-living structure).Methods: In study, we used recombinant interleukin-2 (IL-2) (Ronkoleukin, Russia) oxidizing form and other reagents from Sigma-Aldrich Fluka (USA). For matrix fluid protein concentration 0.6 mg/ml used. solution...

Bacillus coagulans has an advantage over most other bacteria used as probiotics. It occupies intermediate position between the genera Bacillusand Lactobacillus, is a spore-forming that produce lactic acid.This in spores form can tolerate well technology processes, resistant to antibiotics and antiseptics, does not collapse under influence of gastric juice bile. Getting into duodenum, germinate vegetative forms begin vegetation growth, providing probiotic effects. Bacillus coagulansrefers...

Purpose : Wound-healing dipyridamole- and papaverine-based aerosols (D1/D2) as activators of the accumulation cyclic adenosine monophosphate are promising drugs that can accelerate wound healing in processes various origins. Methods: 128 rats were used study, including 38 a pharmacological experiment on model stencil wounds 90 an studied effect spray number CD34 cells blood with chemically induced immunodeficiency. Immunodeficiency was caused by fivefold administration cyclophosphamide...

Nisin is the most well-known representative of subtype A1 lantibiotics. This small (< 5 kDa) peptide ribosomally produced by Gram-positive bacteria belonging to Lactococcus, Streptococcus, Staphylococcus and Blautia species. exhibits antibacterial activity against a broad range may be effective Gram-negative pathogens. For analytical semi-preparative purposes, gradient HPLC often used in acetonitrile concentration from 20 30 % (solvent B) retention time 50 min. In this study optimal...

Enzymatic reactions are the basis of many biotechnological manufacturing and biomedical diagnostic procedures that require effective methods monitoring over reaction course. In current paper, we present results development a new approach within differential microwave dielectrometry method for non-invasive course enzymatic based on complex permittivity changes these reactive mixture solutions in real time at fixed frequency 31.82 GHz. The dynamic studies dielectric parameters selected systems...

Introduction:The paper presents the results of a study on first synthesized benzimidazole derivatives obtained from labile nature carboxylic acids.The synthesis conditions these substances were studied, their structure was proved, and some components found to have sugar-reducing activity model alloxan diabetes in rats. Methods:The used molecular modeling methods such as docking based evolutionary (igemdock), RP_HPLC method monitor reaction, 1H NMR 13C NMR, other organic chemistry confirm...

Актуальность. Проблема лечения персистирующих эрозий роговицы (ПЭР) представляет собой значимую задачу для современной офтальмологии. Местное применение раствора инсулина в качестве дополнительной терапии может значительно улучшить эффективность стандартных методов лечения. Цель. Исследовать местного применения ПЭР сравнении со стандартным лечением. Материал и методы. Исследование проводилось на 9 кроликах (18 глаз), которых была создана модель ПЭР. Глаза были разделены контрольную (9 глаз)...

The aim of the study was to investigate in vitro antimicrobial activity against museum and circulating strains microorganisms fungi an aqueous solution generated by IOON ONE MED device containing atomic hydrogen (active) chlorine at a concentration 1 mg/L as active substances vivo on standardized model purulent peritonitis two variants – prophylactic (neutralization bacteria) therapeutic (effect advanced infection). Methods. Microbiological methods were used new microorganisms, well fungi....

In many clinical cases, the use of probiotic microorganisms is a safer alternative to antibiotic therapy. Spore-forming probiotics, due their high resistance damaging environmental factors, stand out among other beneficial and, therefore, deserve special attention. Determining conditions that enhance inhibitory potential bacilli an important step towards increasing effectiveness against pathogenic bacteria and pathobionts. The aim this study was determine whether spore-forming species:...

Subject and Purpose. This study focuses on the original waveguide-differential dielectrometer designed for complex permittivity measurements of high-loss liquids in microwave range towards determination pharmaceutical ingredient concentrations water solutions at room temperature. The suitability device effectiveness dielectrometry method are tested such ingredients as lincomycin levofloxacin over a wide concentrations. Methods Methodology. main idea consists that propagation coefficients...

Research has been conducted on the chemical and biological properties of combinatorial succinylated derivatives interferon-γ with various levels acylation, which create quasi-life, self-organizing ensembles. As a result research, it established that acylation by succinic anhydride two lysines in structure (Gammaferon) leads to both an increase its affinity cell receptors decrease time maximum effect from 48 hours 15 minutes. Moreover, treatment cells these interferon ensembles led shielding...

Introduction. It's known that some antiseptic sprays based on alcohols can provoke the formation of multi-resistant strains pathogenic microorganisms. In addition, alcoholic antiseptics has a number restrictions to use, for example, they can't be used in even presence of micro-trauma skin, their ingression into body and mucous membranes is unacceptable. Alternative natural antiseptics based colloidal silver or nanoparticles, as well ionic form. However, such low efficacy against...