A5048922044- Immune Cell Function and Interaction
- Intracranial Aneurysms: Treatment and Complications
- CAR-T cell therapy research
- Cerebrovascular and Carotid Artery Diseases
- Acute Ischemic Stroke Management
- Glioma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Vascular Malformations Diagnosis and Treatment
- Brain Metastases and Treatment
- Neurosurgical Procedures and Complications
- T-cell and B-cell Immunology
- Moyamoya disease diagnosis and treatment
- Cerebrospinal fluid and hydrocephalus
- Intracerebral and Subarachnoid Hemorrhage Research
- Venous Thromboembolism Diagnosis and Management
- Inflammatory Biomarkers in Disease Prognosis
- Cardiovascular Health and Disease Prevention
- Anesthesia and Pain Management
- Immunotherapy and Immune Responses
- Traumatic Brain Injury and Neurovascular Disturbances
- Spinal Fractures and Fixation Techniques
- Meningioma and schwannoma management
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Peripheral Artery Disease Management
Nara Medical University
2019-2025
Nara Medical University Hospital
2023
Ohnishi Neurological Center
2015-2022
Cell-based immunotherapy is categorized as a regenerative therapy under the Regenerative Medicine Safety Act in Japan. Natural killer (NK) cell-based considered promising strategy for treating cancer, including glioblastoma (GBM). We previously reported an expansion method highly purified human peripheral blood-derived NK cells using cytokine cocktail. Here, we aimed to establish more efficient cell compared our method. T cell-depleted blood mononuclear (PBMCs) were isolated from three...
Abstract Background Glioblastoma (GBM) is the most common malignant brain tumor and has “immunologically cold” features. Changing GBM to an hot” requires a strong trigger that induces initial immune responses in GBM. Allogeneic natural killer cells (NKCs) have gained considerable attention as promising immunotherapeutic tools against cancer, where gene-edited NKCs would result effective anti-cancer treatment. The present study focused on checkpoint molecule cytokine-inducible SH2-containing...
ABSTRACT This study aimed to evaluate the prognostic value of pre-treatment blood cell counts in patients with brain metastasis (BM) from non-small lung cancer (NSCLC) who were treated using linear accelerator (linac)-based stereotactic radiosurgery (SRS) and fractionated radiotherapy (fSRT) a micro-multileaf collimator. Between January 2011 November 2022, 271 consecutive underwent linac-based SRS/fSRT for BM NSCLC. Thirty insufficient test data during this period excluded analysis....
Background Carotid lipid core plaques (LCPs) have been detected during assessment of carotid artery stenosis using catheter-based near-infrared spectroscopy (NIRS). Treatment with stenting (CAS) dual-layered stents might cause plaque protrusion, and subsequent thromboembolic complications. Objective We aimed to evaluate whether the telescopic (TCS) method suppresses protrusion in patients high LCPs as assessed by NIRS CAS. Methods Participants comprised 63 consecutive LCP, defined maximal...
Periprocedural lipid core plaque (LCP) has been detected in carotid arteries assessed by catheter-based near-infrared spectroscopy (NIRS). High LCP is associated with cerebral embolism after artery stenting (CAS) using a first-generation stent. We aimed to evaluate whether dual-layered stents reduce embolic infarcts patients high and change of signal as NIRS during CAS.Participants comprised 210 consecutive undergoing CAS. The study was divided into two distinct periods, closed-cell used the...
Enhancing immune cell functions in tumors remains a major challenge cancer immunotherapy. Natural killer cells (NK) are innate effector with broad cytotoxicity against tumors. Accordingly, NK ideal candidates for immunotherapy, including glioblastoma (GBM). Hypoxia is common feature of solid tumors, and tumor normal adapt to the microenvironment by upregulating transcription factor hypoxia-inducible (HIF)-1α, which can be detrimental anti-tumor function, that cells. We knocked out HIF-1α...
Glioblastoma (GBM) is the leading malignant intracranial tumor and associated with a poor prognosis. Highly purified, activated natural killer (NK) cells, designated as genuine induced NK cells (GiNKs), represent promising immunotherapy for GBM. We evaluated anti-tumor effect of GiNKs in association programmed death 1(PD-1)/PD-ligand 1 (PD-L1) immune checkpoint pathway. determined level PD-1 expression, receptor known to down-regulate response against malignancy, on GiNKs. PD-L1 expression...
Innate immune cells are important in tumor immunotherapy. Natural killer (NKCs) also categorized as innate and can control growth metastatic spread. Glioblastoma (GBM) is the most common aggressive primary brain adults. NKC-based immunotherapy a promising treatment strategy against GBM. We previously reported feeder-free expansion system that yielded large-scale highly purified cytotoxic NKCs derived from human cord blood (CB). In present study, we performed comprehensive genomic analyses of...
Angiocentric glioma (AG) is an extremely rare tumor that often develops in adolescents. Awake surgery for AG occurring the eloquent area has not been reported to date. We report a case involving right-handed 15-year-old boy with AG. He presented first-time generalized tonic-clonic seizure and was rushed local hospital. CT of head indicated left frontal low-density mass no calcification. subsequently referred our Comparison scan obtained two years prior due mild trauma lesion showed trend...
Background: Intracranial vertebral artery dissecting aneurysm (VADA) is rare and shows high morbidity mortality rates when the ruptures. Endovascular treatment for VADA one of optimal treatments, but dominant side VA its branches or perforators need to be preserved. We report a novel successful stent-assisted coil embolization technique using low-profile visualized intraluminal support (LVIS) stent, with five technical notes in three consecutive cases unruptured (VADA). Case Description:...
Irradiated murine induced-pluripotent stem cells (iPSCs) elicit the antitumor response in vivo. However, it is unclear whether human iPSCs would effects. In present study, we investigated capability of iPSC lysate (iPSL)-pulsed dendritic (DCs) (iPSL/DCs) to induce cancer-responsive cytotoxic T lymphocytes (CTLs) vitro. and DCs were induced from peripheral blood mononuclear isolated a leukocyte antigen (HLA)-A33 homozygous donor. The iPSL was pulsed with immature DCs, which then stimulated...
Natural killer cells (NKCs) are immune that can attack cancer through the direct recognition of ligands without prior sensitization. Cord blood-derived NKCs (CBNKCs) represent a promising tool for allogenic NKC-based immunotherapy. Efficient NKC expansion and decreased T cell inclusion crucial success allogeneic immunotherapy inducing graft-versus-host reactions. We previously established an efficient ex vivo system consisting highly purified-NKCs derived from human peripheral blood. Herein,...
Stent apposition to the vessel wall and in-stent neointimal formation after stent-assisted coil embolization for intracranial aneurysm are important factors associated with postoperative thromboembolic complications. No assessment methods have been established depict 3-dimensional (3D) all-round formation.To demonstrate superiority of Dyna-3D imaging as a modality ISNF in comparison conventional two-dimensional digital subtraction angiography (2D-DSA).Consecutive patients who underwent...
Glioblastoma is a type of intracranial malignancy. Shikonin, Chinese traditional medicine, has been shown to have anti-tumor efficacy toward human glioblastoma cells in vitro. However, shikonin cannot easily cross the blood-brain barrier. To address this issue, we evaluated effects direct infusion vivo orthotopic syngeneic murine models using C57BL/6 mice.The cytotoxic against cells, SB28 and CT-2A, were reported resistance temozolomide, an allophycocyanin-conjugated annexin V propidium...
Fenestration of the middle cerebral artery (MCA) is a rare anatomic variant, and lenticulostriate arteries (LSAs) often arise from superior limb fenestrated segment. A case acute occlusion MCA that successfully underwent mechanical thrombectomy presented. Digital subtraction angiography performed for 73-year-old man with left hemiparesis showed poor visualization upper half right M1 segment maintenance antegrade peripheral circulation territory, was using stent retriever intravenous...