A5059857599- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Insect symbiosis and bacterial influences
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Parasites and Host Interactions
- Bacteriophages and microbial interactions
- Parasitic Diseases Research and Treatment
- Tuberculosis Research and Epidemiology
- Infective Endocarditis Diagnosis and Management
- Advanced biosensing and bioanalysis techniques
- Vector-Borne Animal Diseases
- Genomics and Phylogenetic Studies
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Streptococcal Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
- Biochemical and Molecular Research
- Parasitic Infections and Diagnostics
- COVID-19 diagnosis using AI
- Helminth infection and control
- Viral Infections and Immunology Research
Instituut voor Tropische Geneeskunde
2015-2025
Médecins Sans Frontières
2025
Médecins Sans Frontières
2021-2023
Instituto de Medicina Tropical
2021
Universidad Nacional
2014
Rega Institute for Medical Research
2006-2012
KU Leuven
2008-2012
University of California, Santa Barbara
2012
Research Institute for Tropical Medicine
2011
Province of Antwerp
2011
Background A century after its discovery, Chagas disease still represents a major neglected tropical threat. Accurate diagnostics tools as well surrogate markers of parasitological response to treatment are research priorities in the field. The purpose this study was evaluate performance PCR methods detection Trypanosoma cruzi DNA by an external quality evaluation. Methodology/Findings An international collaborative launched expert laboratories from 16 countries. Currently used strategies...
Abstract Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) are dominated isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence ST313 sublineage II.1 Democratic Republic Congo. Sublineage exhibits extensive drug resistance, involving combination multidrug extended spectrum β-lactamase production and azithromycin resistance. lineage...
The C-terminal variants G1 and G2 of apolipoprotein L1 (APOL1) confer human resistance to the sleeping sickness parasite Trypanosoma rhodesiense, but they also increase risk kidney disease. APOL1 APOL3 are death-promoting proteins that partially associated with endoplasmic reticulum Golgi membranes. We report in podocytes, either helix truncation (APOL1Δ) or deletion (APOL3KO) induces similar actomyosin reorganization linked inhibition phosphatidylinositol-4-phosphate [PI(4)P] synthesis by...
Important control efforts have led to a significant reduction of the prevalence human African trypanosomiasis (HAT) in Côte d'Ivoire, but disease is still present several foci. The existence an animal reservoir Trypanosoma brucei gambiense may explain persistence these foci where breeding important source income (AAT) unknown. aim this study was identify trypanosome species circulating domestic animals both Bonon and Sinfra HAT endemic foci.552 (goats, pigs, cattle sheep) were included....
ABSTRACT Human African trypanosomiasis (HAT) or sleeping sickness is a neglected disease that affects poor rural populations across sub-Saharan Africa. Confirmation of diagnosis based on detection parasites in either blood lymph by microscopy. Here we present the development and first-phase evaluation simple rapid test (HAT-PCR-OC [human trypanosomiasis-PCR-oligochromatography]) for amplified Trypanosoma brucei DNA. PCR products are visualized dipstick through hybridization with...
Background. Definite diagnosis of Leishmania infections is based on demonstration the parasite by microscopic analysis tissue biopsy specimens or aspirate samples. However, microscopy generally shows low sensitivity and requires invasive sampling.
Summary Objective To develop a new PCR for Leishmania detection and to estimate its diagnostic accuracy in visceral leishmaniasis (VL) endemic area. Methods After providing the proof‐of‐concept, was estimated on blood from 247 non‐endemic control persons bone marrow 173 confirmed VL, 39 probable VL 87 non‐VL patients south‐eastern Nepal. Results The showed specificity of 99.64% [95% confidence interval (CI): 98.93–100%) controls sensitivity 92.1% (95% CI: 87.6–96.6%) 92.9% 89–96.8%)...
Background The polymerase chain reaction (PCR) has been proposed for diagnosis, staging and post-treatment follow-up of sleeping sickness but no large-scale clinical evaluations its diagnostic accuracy have taken place yet. Methodology/Principal Findings An 18S ribosomal RNA gene targeting PCR was performed on blood cerebrospinal fluid (CSF) 360 T. brucei gambiense patients 129 endemic controls from the Democratic Republic Congo. Sensitivity specificity (with 95% confidence intervals)...
Surra, a vector borne disease caused by Trypanosoma (T.) evansi, affects the health, productivity and working capacity of camels. Since clinical signs are not pathognomonic, diagnosis must be confirmed laboratory methods. This is first study on prevalence surra in Cholistan Desert, Pakistan using broad variety diagnostic tests thereby emphasizing it as risk for dromedaries Pakistan.In cross sectional study, 1005 dromedary camels from three districts Desert were sampled to assess...
Background Bacterial bloodstream infection (bBSI) is one of the leading causes death in critically ill patients and accurate diagnosis therefore crucial. We here report a 16S metagenomics approach for diagnosing understanding bBSI. Methodology/Principal Findings The proof-of-concept was delivered 75 children (median age 15 months) with severe febrile illness Burkina Faso. Standard blood culture malaria testing were conducted at time hospital admission. done retrospectively duplicate on all...
Salmonella Typhimurium and Enteritidis are major causes of bloodstream infection in children sub-Saharan Africa. This study assessed evidence for their zoonotic versus human reservoir.Index patients were with blood culture confirmed recruited during a microbiological surveillance Nanoro, rural Burkina between May 2013 August 2014. After consent, households visited. Stool from household members livestock (pooled samples per species) as well drinking water cultured Salmonella. Isolates...
Background Non-typhoidal Salmonella (NTS) are a major cause of bloodstream infection (BSI) in sub-Saharan Africa. This study aimed to assess its longitudinal evolution as BSI, serotype distribution and antibiotic resistance pattern Kisantu, DR Congo. Methods As part national surveillance network, blood cultures were sampled patients with suspected BSI admitted Kisantu referral hospital from 2015–2017. Blood worked-up according international standards. Results compared similar data 2007...
Background PCR has evolved into one of the most promising tools for T. cruzi detection in diagnosis and control Chagas disease. However, general use technique is hampered by its complexity lack standardization. Methodology We here present development phase I evaluation OligoC-TesT, a simple standardized dipstick format amplified DNA. The specificity sensitivity assay were evaluated on blood samples from 60 non-endemic 48 endemic persons biological 33 patients, 7 reservoir animals, 14 vectors...
Non-malaria febrile illnesses such as bacterial bloodstream infections (BSI) are a leading cause of disease and mortality in the tropics. However, there no reliable, simple diagnostic tests for identifying BSI or other severe non-malaria illnesses. We hypothesized that different infectious agents responsible illness would impact on host metabolome ways, investigated potential plasma metabolites diagnosis illness.We conducted comprehensive mass-spectrometry based metabolomics analysis 61...
Trypanosomes cause a variety of diseases in man and domestic animals Africa, Latin America, Asia. In the Trypanozoon subgenus, Trypanosoma brucei gambiense rhodesiense human African trypanosomiasis, whereas brucei, evansi, equiperdum are responsible for nagana, surra, dourine animals, respectively. The genetic relationships between T. evansi other species remain unclear because majority phylogenetic analyses has been based on only few genes. this study, we have conducted analysis genome-wide...
Salmonella enterica subsp. contains more than 2,600 serovars of which four are major medical relevance for humans. While the typhoidal (Typhi and Paratyphi A) human-restricted cause enteric fever, non-typhoidal (Typhimurium Enteritidis) have a broad host range predominantly gastroenteritis.We compared core proteomes Typhi, A, Typhimurium Enteritidis using contemporary proteomics. For each serovar, five clinical isolates (covering different geographical origins) one reference strain were...
Background The GeneXpert diagnostic platform from the US based company Cepheid is an automated molecular device that performs sample preparation and pathogen detection within a single cartridge-based assay. devices can enable diagnosis at district level without need for fully equipped clinical laboratories, are simple to use, offer rapid results. Due these characteristics, now widely used in low- middle-income countries of diseases such as TB HIV. Assays SARS-CoV-2 also being rolled out. We...