Abstract In contrast to gene-for-gene disease resistance, nonhost resistance governs defense responses a broad range of potential pathogen species. To identify specific genes involved in the signal transduction cascade associated with we used virus-induced gene-silencing screen Nicotiana benthamiana, and identified peroxisomal enzyme glycolate oxidase (GOX) as an essential component resistance. GOX-silenced N. benthamiana Arabidopsis thaliana GOX T-DNA insertion mutants are compromised for...

SUMMARY We have developed a compendium and interactive platform, named Stress Combinations their Interactions in Plants Database (SCIPDb; http://www.nipgr.ac.in/scipdb.php ), which offers information on morpho‐physio‐biochemical (phenome) molecular (transcriptome metabolome) responses of plants to different stress combinations. SCIPDb is plant informatics hub for data mining phenome, transcriptome, trait‐gene ontology, data‐driven research advancing mechanistic understanding combined...

Abstract Drought dynamically influences the interactions between plants and pathogens, thereby affecting disease outbreaks. Understanding intricate mechanistic aspects of multiscale among plants, environment—known as triangle—is paramount for enhancing climate resilience crop plants. In this review, we systematically compile comprehensively analyse current knowledge on influence drought severity plant diseases. We emphasise that studying these stresses in isolation is not sufficient to...

Abstract Bacterial pathogens colonize a host plant by growing between the cells utilizing nutrients present in apoplastic space. While successful manipulate cell membrane to retrieve more from cell, counteracting defense mechanism against nonhost restrict nutrient efflux into apoplast is not clear. To identify genes involved resistance bacterial pathogens, we developed virus-induced gene-silencing-based fast-forward genetics screen Nicotiana benthamiana. Silencing of N. benthamiana SQUALENE...

Abstract Drought stress and pathogen infection simultaneously occur in the field. In this study, interaction of these two stresses with chickpea, their individual combined effect net impact on plant growth yield traits were systematically assessed under field confined pot experiments. The experiments conducted for four consecutive years from 2014–15 to 2017–18 at different locations India. Different irrigation regimes maintained impose mild severe drought stress, natural incidence was...

Global transcriptome studies demonstrated the existence of unique plant responses under combined stress which are otherwise not seen during individual stresses. In order to combat plants use signaling pathways and 'cross talk' mediated by hormones involved in growth related processes. However, interactions among hormones' stressed yet known. Here we studied dynamics different drought pathogen infection Arabidopsis thaliana liquid chromatography-mass spectrometry (LC-MS) based profiling. Our...

Virus-induced gene silencing (VIGS) is one of the commonly used RNA methods in plant functional genomics. It widely known that VIGS can occur for about 3 weeks. A few reports show duration be prolonged up to months. Increasing endogenous and developing a method nonintegration-based persistent progeny seedlings will widen application VIGS. We three marker genes provoke visible phenotypes plants upon study persistence transmittance two species, Nicotiana benthamiana tomato. Tobacco rattle...

ABSTRACT Non‐host disease resistance involves the production of hypersensitive response (HR), a programmed cell death (PCD) that occurs at site pathogen infection. Plant mitochondrial reactive oxygen species (ROS) and red‐ox changes play major role in regulating such death. Proline catabolism reactions, especially pyrroline‐5‐carboxylate (P5C) accumulation, are known to produce ROS contribute Here we studied important genes related proline synthesis defence against host non‐host strains...

Finger millet is susceptible to abiotic stresses, especially drought and salinity stress, in the field during seed germination early stages of seedling development. Therefore developing stress tolerant finger plants combating drought, associated oxidative these two growth important. Cellular protection through osmotic adjustment efficient free radical scavenging ability are important components tolerance mechanisms plants. Mannitol, an osmolyte, known scavenge hydroxyl radicals generated...

Acclimation of plants with an abiotic stress can impart tolerance to some biotic stresses. Such a priming response has not been widely studied. In particular, little is known about enhanced defense capacity drought acclimated fungal and bacterial pathogens. Here we show that prior acclimation in Nicotiana benthamiana imparts necrotrophic fungus, Sclerotinia sclerotiorum, also hemi-biotrophic pathogen, Pseudomonas syringae pv. tabaci. S. sclerotiorum inoculation on N. lead less...

Abstract Drought transcriptome analysis of finger millet ( Eleusine coracana ) by cDNA subtraction identified drought responsive genes that have a potential role in tolerance. Through virus-induced gene silencing (VIGS) related crop species, maize Zea mays ), several genes, including G-BOX BINDING FACTOR 3 GBF3 were as candidate stress response and the tolerance was studied Arabidopsis thaliana . Overexpression both EcGBF3 AtGBF3 A. resulted improved to osmotic stress, salinity addition...

Characterizing the molecular mechanism involved in nonhost disease resistance is important to understand adaptations of plant-pathogen interactions. In this study, virus-induced gene silencing (VIGS)-based forward genetics screen was utilized identify genes Nicotiana benthamiana. Genes encoding ribosomal proteins, RPL12 and RPL19, were identified screening. These when silenced N. benthamiana caused a delay bacteria induced hypersensitive response (HR) with concurrent increase bacterial...

Virus-induced gene silencing (VIGS) is a rapid and robust method for determining studying the function of plant genes or expressed sequence tags (ESTs). However, only few species are amenable to VIGS. There need systematic study identify VIGS-efficient determine extent homology required between heterologous their endogenous orthologs silencing. Two approaches were used. First, phytoene desaturase (PDS) was studied in various Solanaceous using Nicotiana benthamiana NbPDS sequences. In second...

Peanut, found to be relatively drought tolerant crop, has been the choice of study characterize genes expressed under gradual water deficit stress. Nearly 700 were identified enriched in subtractive cDNA library from process stress adaptation. Further, expression inducible related various signaling components and gene sets involved protecting cellular function described based on dot blot experiments. Fifty (25 regulators 25 functional genes) selected experiments tested for their...

NAC (NAM, ATAF1-2, and CUC2) proteins constitute one of the largest families plant-specific transcription factors have been shown to be involved in diverse plant processes including growth, development, stress-tolerance. In this study, a stress-responsive gene, EcNAC1, was isolated from subtracted stress cDNA library generated drought adapted crop, finger millet, characterized for its role The expression analysis showed that EcNAC1 highly induced during water-deficit salt stress. shares high...