A5040476997- Kruppel-like factors research
- Head and Neck Cancer Studies
- Transplantation: Methods and Outcomes
- Lung Cancer Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Organ Transplantation Techniques and Outcomes
- Fibroblast Growth Factor Research
- Salivary Gland Tumors Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Cancer-related gene regulation
- AI in cancer detection
- Lung Cancer Treatments and Mutations
- Brain Tumor Detection and Classification
- Renal Transplantation Outcomes and Treatments
- Developmental Biology and Gene Regulation
- Neuroendocrine Tumor Research Advances
- Congenital heart defects research
- Pluripotent Stem Cells Research
- TGF-β signaling in diseases
- Medical Imaging and Pathology Studies
- Cervical Cancer and HPV Research
- Gastric Cancer Management and Outcomes
- Head and Neck Surgical Oncology
- Colorectal Cancer Screening and Detection
- Mechanical Circulatory Support Devices
Washington University in St. Louis
2015-2024
Barnes-Jewish Hospital
2015-2018
Jewish Hospital
2015
University of Vermont
2014
Massachusetts General Hospital
2014
Virginia Commonwealth University
2014
Mount Sinai Hospital
2014
University of Nebraska Medical Center
2002-2007
University of Nebraska–Lincoln
2001
Molecular testing of cancer is increasingly critical to medicine. Next-generation sequencing (NGS) provides comprehensive, unbiased, and inexpensive mutation analysis multiple genes with a single test. However, the authors' knowledge, usefulness NGS in fine-needle aspiration (FNA) specimens, which may be only specimens available, unknown. Non-small cell lung (NSCLC) an ideal model evaluate cytopathologic applications because FNA used for diagnosis staging specific molecular therapeutic...
Brain metastases can occur in nearly half of patients with early and locally advanced (stage I-III) non-small cell lung cancer (NSCLC). There are no reliable histopathologic or molecular means to identify those who likely develop brain metastases. We sought determine if deep learning (DL) could be applied routine H&E-stained primary tumor tissue sections from stage I-III NSCLC predict the development metastasis. Diagnostic slides 158 followed for at least 5 years (Met
Recent studies have identified large sets of genes in embryonic stem and embryonal carcinoma cells that are associated with the transcription factors Sox2 Oct-3/4. Other shown Oct-3/4 work together cooperatively to stimulate their own as well a network required for embryogenesis. Moreover, small changes levels Sox2:Oct-3/4 target alter fate cells. Although positive feedforward feedback loops been proposed explain activation these genes, little is known about mechanisms prevent...
BACKGROUND Rapid on‐site evaluation (ROSE) for endoscopic ultrasound‐guided fine‐needle aspiration (EUS‐FNA) biopsy of the pancreas provides immediate feedback regarding cellular adequacy to aid in obtaining a definitive diagnosis and has potential avoid repeat procedures. The objective current study was measure impact ROSE service on incidence EUS FNA METHODS Over consecutive 3‐year period, pathology database at Washington University Medical Center searched patients with both an initial...
BACKGROUND Endobronchial ultrasound guided (EBUS) fine‐needle aspiration (FNA) biopsy has become widely used to evaluate patients with thoracic abnormalities. Rapid on‐site evaluation (ROSE) can provide the bronchoscopist immediate findings during procedure. This study examines EBUS FNA procedures and without ROSE, investigates impact of ROSE service on procedure laboratory resource utilization. METHODS The cytopathology database at Washington University Medical Center, St. Louis, Missouri,...
BACKGROUND Human papillomavirus (HPV)‐related oropharyngeal squamous cell carcinoma (SCC) is a unique form of that important to identify for prognosis and treatment. Immunohistochemistry (IHC) p16 (also known as cyclin‐dependent kinase inhibitor 2A, multiple tumor suppressor 1) used surrogate marker transcriptionally active, high‐risk HPV. The primary objective this study was correlate IHC blocks from fine‐needle aspirations (FNAs) with surgical pathology specimens HPV‐related SCC. METHODS...
The development of donor-specific antibodies (DSA) after lung transplantation is common and results in adverse outcomes. In kidney transplantation, Belatacept has been associated with a lower incidence DSA, but experience limited. We conducted two-center pilot randomized controlled trial de novo immunosuppression to assess the feasibility conducting pivotal trial. Twenty-seven participants were Control (Tacrolimus, Mycophenolate Mofetil, prednisone, n = 14) or Belatacept-based Belatacept,...
Cytology is the study of whole cells in diagnostic pathology. Unlike standard histologic thinly sliced specimens, cytologic preparations consist where commonly cluster and aggregate. As such, cytology are generally much thicker than slides, resulting large patches defocus when examined under microscope. A aggregate often cannot be viewed focus together, requiring pathologists to continually manipulate focal plane, complicating task accurately assessing entire cellular thus making a...
Background. Chronic lung allograft dysfunction (CLAD) is the leading cause of death beyond first year after transplantation. The development donor-specific antibodies (DSA) a recognized risk factor for CLAD. Based on experience in kidney transplantation, we hypothesized that belatacept, selective T-cell costimulatory blocker, would reduce incidence DSA which may ameliorate Methods. We conducted pilot randomized controlled trial (RCT) at 2 sites to assess feasibility and inform design...
Early-onset colorectal cancer has been on the rise in Western populations. Here, we compare patient characteristics between those with early- (<50 years) vs. late-onset (≥50 disease a large multinational cohort of patients (n = 2193). We calculated descriptive statistics and assessed associations clinicodemographic factors age onset using mutually-adjusted logistic regression models. Patients were average 60 years old, BMI 29 kg/m2, 52% colon cancers, 21% early-onset, presented stage II or...
Distal enhancers commonly regulate gene expression. However, the mechanisms of transcriptional mediation by distal remain largely unknown. To better understand enhancer-mediated transcription, we examined regulation theFGF-4 gene. The FGF-4 is regulated during early development a powerful enhancer located downstream promoter in exon 3. Sox-2 and Oct-3 bind to are required for activation Previously, implicated co-activator p300 as mediator Sox-2/Oct-3 synergistic heterologous promoter,...
Specific subclassification of pulmonary non-small cell carcinoma (NSCCA) is clinically necessary, and the aim this study to examine utilization p40 (ΔNp63) in fine-needle aspiration (FNA) biopsy for lung NSCCA.Database files Washington University Medical Center were searched. Patients who underwent endobronchial ultrasound CT FNA a primary neoplasia selected immunohistochemistry (IHC) was performed. A panel markers utilized, including p40, p63, cytokeratin (CK) 5/6, thyroid transcription...
NF-Y is a bifunctional transcription factor capable of activating or repressing transcription. specifically recognizes CCAAT box motifs present in many eukaryotic promoters. The mechanisms involved regulating its activity are poorly understood. Previous studies have shown that the FGF-4 promoter regulated positively by and embryonal carcinoma (EC) cells where distal enhancer gene active. Here, we demonstrate functions as negative cis-regulatory element when elements disrupted, after EC...
Abstract Transcription factors Oct‐3/4 and Sox2 behave as global regulators during mammalian embryogenesis. They work together by binding co‐operatively to closely spaced HMG POU motifs ( / cassettes). Recently, it was suggested that a critical Sox2:Oct‐3/4 target gene, FGF‐4 , is expressed at lower levels in P19 than F9 embryonal carcinoma (EC) cells, due of cells. We tested this possibility better understand how expression modulated development. Although we found EC cells express ∼10‐fold...
Abstract Previous studies have demonstrated that differentiation of murine embryonal carcinoma (EC) cells leads to the appearance high affinity receptors for transforming growth factor‐β (TGF‐β). Subsequently, it was F9 EC increases in transcription type II TGF‐β‐receptor gene ( TβR‐II ) and significant steady‐state levels mRNA. Analysis human promoter F9‐differentiated identified several cis ‐regulatory elements influence activity promoter, including a CRE/ATF site CCAAT box motif. In work...
Abstract Previous studies have shown that transcription of the fibroblast growth factor‐4 ( FGF‐4 ) gene by early embryonic cells is dependent upon a powerful distal enhancer located 3 kb downstream start site within untranslated region last exon. The factors Sox‐2 and Oct‐3 cooperatively bind to critical cis ‐regulatory elements synergistically activate transcription. Moreover, co‐activator p300 can mediate synergistic activity Oct‐3, associates with in vivo. Embryonal carcinoma (EC) been...