A5063081039- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Advanced Chemical Sensor Technologies
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Nitric Oxide and Endothelin Effects
- Porphyrin and Phthalocyanine Chemistry
- Nanoplatforms for cancer theranostics
- Luminescence and Fluorescent Materials
- Water Quality Monitoring and Analysis
- Electrochemical Analysis and Applications
- Biosensors and Analytical Detection
- ATP Synthase and ATPases Research
- Advanced Fluorescence Microscopy Techniques
- Adipose Tissue and Metabolism
- Meat and Animal Product Quality
- Metabolomics and Mass Spectrometry Studies
- Gas Sensing Nanomaterials and Sensors
- Optical Imaging and Spectroscopy Techniques
- Microfluidic and Capillary Electrophoresis Applications
- bioluminescence and chemiluminescence research
- Conducting polymers and applications
- Photodynamic Therapy Research Studies
University College Cork
2015-2024
Institute of Biomedical Chemistry
2007-2015
Bellingham Technical College
2015
National University of Ireland
1998-2012
In-Q-Tel
2011
Masaryk University
2010
Lomonosov Moscow State University
2009
University of Turku
1998-2002
Russian Research Center for Molecular Diagnostics and Therapy
1992-1997
University of Graz
1997
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhosphorescent Complexes of Porphyrin Ketones: Optical Properties and Application to Oxygen SensingDmitri B. Papkovsky, Gelii V. Ponomarev, Wolfgang. Trettnak, Paul. O'LearyCite this: Anal. Chem. 1995, 67, 22, 4112–4117Publication Date (Print):November 15, 1995Publication History Published online1 May 2002Published inissue 15 November 1995https://pubs.acs.org/doi/10.1021/ac00118a013https://doi.org/10.1021/ac00118a013research-articleACS...
Mitochondrial biogenesis occurs in response to increased cellular ATP demand. The mitochondrial electron transport chain requires molecular oxygen produce ATP. Thus, generation after results demand that must be matched by a corresponding increase supply. We found overexpression of peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha), which increases primary skeletal muscle cells, leads expression cohort genes known regulated the dimeric hypoxia-inducible factor...
A new intracellular O2 (icO2) sensing probe is presented, which comprises a nanoparticle (NP) formulation of cationic polymer Eudragit RL-100 and hydrophobic phosphorescent dye Pt(II)-tetrakis(pentafluorophenyl)porphyrin (PtPFPP). Using the time-resolved fluorescence (TR-F) plate reader set-up, cell loading was investigated in detail, particularly effects concentration, time, serum content medium, type, density, etc. The use fluorescent analogue conjunction with confocal microscopy flow...
Hypoxia is one of the features poorly vascularised areas solid tumours but cancer cells can survive in these despite low oxygen tension. The adaptation to hypoxia requires both biochemical and genetic responses that culminate a metabolic rearrangement counter-balance decrease energy supply from mitochondrial respiration. understanding adaptations under could reveal novel pathways that, if targeted, would lead specific death hypoxic regions. In this study, we developed metabolomic analyses...
The G2019S leucine rich repeat kinase 2 (LRRK2) mutation is the most common genetic cause of Parkinson's disease (PD), clinically and pathologically indistinguishable from idiopathic PD. Mitochondrial abnormalities are a feature in PD pathogenesis we have investigated impact mutant LRRK2 expression on mitochondrial bioenergetics. protein was detected fibroblasts lymphoblasts at levels higher than those observed mouse brain. presence did not influence fibroblasts. However, both fibroblast...
Abstract Monitoring cell and tissue oxygenation is important for the analysis of development differentiation, mitochondrial function, common (patho)physiological conditions such as ischemia, cancer, neurodegenerative disorders. A number materials sensing cellular oxygen (O 2 ) by optical means have been described in recent years, but diverse range biological models measurement tasks demands more versatile, flexible, simple O sensors. new cell‐penetrating phosphorescent nanosensor material...
High brightness, chemical and photostability, tunable characteristics, spectral surface properties are important attributes for nanoparticle probes designed live cell imaging. We describe a class of nanoparticles high-resolution imaging O2 that consists substituted conjugated polymer (polyfluorene or poly(fluorene-alt-benzothiadiazole)) acting as FRET antenna fluorescent reference with covalently bound phosphorescent metalloporphyrin (PtTFPP, PtTPTBPF). The prepared from such copolymers by...
The gastrointestinal microbiota is emerging as a unique and inexhaustible source for metabolites with potential to modulate G-protein coupled receptors (GPCRs). ghrelin receptor [growth hormone secretagogue (GHSR)-1a] GPCR expressed throughout both the gut brain plays crucial role in maintaining energy balance, metabolism, central modulation of food intake, motivation, reward, mood. To date, few studies have investigated its signaling. Here we investigate ability short-chain fatty acids...
Oxygen and glucose metabolism play pivotal roles in many (patho)physiological conditions. In particular, oxygen deprivation (OGD) during ischemia stroke results extensive tissue injury cell death. Using time-resolved ribosome profiling, we assess gene expression levels a neural line, PC12, the first hour of OGD. The most substantial alterations are seen to occur within 20 minutes While transcription only 100 genes is significantly altered one OGD, translation response affects approximately...
PtPFPP-carbohydrate conjugates are promising O<sub>2</sub>probes for 3D PLIM imaging of live spheroids and brain explants.
Stem cells and the niche in which they reside feature a complex microenvironment with tightly regulated homeostasis, cell-cell interactions dynamic regulation of metabolism. A significant number organoid models has been described over last decade, yet few methodologies can enable single cell level resolution analysis stem metabolic demands, real-time without perturbing integrity. Here, we studied redox metabolism Lgr5-GFP intestinal organoids by two emerging microscopy approaches based on...
Mitochondrial dysfunction is a common mechanism of drug-induced toxicity. Early identification new chemical entities (NCEs) that perturb mitochondrial function significant importance to avoid attrition in later stages drug development. One the most informative ways assessing by measuring oxygen consumption. However, conventional polarographic method consumption not amenable high sample throughput or automation. We present an alternative, low-bulk, high-throughput approach analysis...
Mutations in the gene encoding Krebs cycle enzyme fumarate hydratase (FH) predispose to hereditary leiomyomatosis and renal cell cancer affected individuals. FH-associated neoplasia is characterized by defective mitochondrial function upregulation of transcriptional pathways mediated hypoxia-inducible factor (HIF), although whether what means these processes are linked has been disputed. We analysed HIF pathway Fh1−/− mouse embryonic fibroblasts (MEFs), FH-defective neoplastic tissues MEFs...
The supply of oxygen (O(2)) to respiring tissue, cells, and mitochondria regulates metabolism, gene expression, cell fate. Depending on the type mitochondrial function, O(2) gradients between extra- intracellular compartments may vary play important physiological roles such as regulation activity prolyl hydroxylases adaptive responses hypoxia. Here we present a new methodology for analysis localized in cultures adherent using three phosphorescent Pt-porphyrin based probes with different...
Abstract Ir III –porphyrins are a relatively new group of phosphorescent dyes that have potential for oxygen sensing and labeling biomolecules. The requirement two axial ligands the ion permits simple linkage biomolecules by one‐step ligand‐exchange reaction, example, using precursor carbonyl chloride complexes peptides containing histidine residue(s). Using this approach, we produced three –octaethylporphyrin with cell‐penetrating (Ir1 Ir2) tumor‐targeting (Ir3) studied their photophysical...