A5063474837- Geomagnetism and Paleomagnetism Studies
- Magnetic and Electromagnetic Effects
- Calcium Carbonate Crystallization and Inhibition
- Fungal and yeast genetics research
- Viral Infectious Diseases and Gene Expression in Insects
- Characterization and Applications of Magnetic Nanoparticles
- Polymer Surface Interaction Studies
- Drilling and Well Engineering
- Tunneling and Rock Mechanics
- Electrochemical Analysis and Applications
- Forensic Toxicology and Drug Analysis
- Iron Metabolism and Disorders
- Geoscience and Mining Technology
- Electrospun Nanofibers in Biomedical Applications
- Sphingolipid Metabolism and Signaling
- Polyamine Metabolism and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Rock Mechanics and Modeling
- Pharmacological Effects and Assays
- Neonatal Health and Biochemistry
- Diatoms and Algae Research
- Electrochemical sensors and biosensors
- Fungal Biology and Applications
- Attention Deficit Hyperactivity Disorder
- Advanced Computational Techniques and Applications
Iowa State University
2013-2022
Ames National Laboratory
2013-2022
Tianjin Children's Hospital
2020-2021
Roy J. Carver Charitable Trust
2016-2019
Nankai University
2008-2012
Institute of Rock and Soil Mechanics
2004
Hunan Hydro&Power Design Institute
2004
Hydrophobins are a group of low-molecular-mass, cysteine-rich proteins that have unusual biophysical properties. They highly surface-active and can self-assemble at hydrophobic–hydrophilic interfaces, forming surface layers able to reverse the hydropathy surfaces. Here we describe novel hydrophobin from edible mushroom Grifola frondosa, which was named HGFI belongs class I. The gene identified during sequencing random clones cDNA library, corresponding protein isolated as hot SDS-insoluble...
A common feature of biomineralization proteins is their self-assembly to produce a surface consistent in size with the inorganic crystals that they produce. Mms6, small protein 60 amino acids from Magnetospirillum magneticum strain AMB-1 promotes vitro growth superparamagnetic magnetite nanocrystals, assembles aqueous solution form spherical micelles could be visualized by TEM and AFM. The results reported here are view N C-terminal domains interact each other within one polypeptide chain...
Magnetotactic bacteria that produce magnetic nanocrystals of uniform size and well-defined morphologies have inspired the use biomineralization protein Mms6 to promote formation in vitro. Small angle X-ray scattering (SAXS) studies physiological solutions reveal forms compact globular three-dimensional (3D) micelles (approximately 10 nm diameter) are, a large extent, independent concentration. In presence iron ions solutions, general micellar morphology is preserved, however, with...
Hydrophobins are small fungal proteins that self-assemble spontaneously at hydrophilic-hydrophobic interfaces and change the polar nature of surfaces to which they attach. A new hydrophobin gene hgfI was identified recently from edible mushroom Grifola frondosa. In this paper, cloning, expression, purification, polyclonal antibody preparation HGFI were described. The cloned into pET-28a expression plasmid EcoRI NdeI restriction sites then transformed Escherichia coli BL21 strain. SDSPAGE...
Surface-sensitive X-ray scattering and spectroscopy techniques reveal significant adsorption of iron ions iron-hydroxide (Fe(III)) complexes to a charge-neutral zwitterionic template phosphatidylcholine (PC). The PC is formed by Langmuir monolayer dipalmitoyl-PC (DPPC) that spread on the surface 2 40 μM FeCl3 solutions at physiological levels KCl (100 mM). At Fe(III) as many ∼3 atoms are associated with each group. Grazing incidence diffraction measurements indicate disruption in in-plane...
Magnetosomes of magnetotactic bacteria consist magnetic nanocrystals with defined morphologies enclosed in vesicles originated from cytoplasmic membrane invaginations. Although many proteins are involved creating magnetosomes, a single magnetosome protein, Mms6 Magnetospirillum magneticum strain AMB-1, can direct the crystallization magnetite nanoparticles vitro. The vivo role formation is debated, and observation that binds Fe3+ more tightly than Fe2+ raises question how, environment...
Magnetotactic bacteria produce magnetic nanocrystals with uniform shapes and sizes in nature, which has inspired vitro synthesis of uniformly sized magnetite under mild conditions. Mms6, a biomineralization protein from magnetotactic hydrophobic N-terminal domain hydrophilic C-terminal domain, can promote formation well-defined shape size gels Here we investigate the role surface hydrophobicity on ability Mms6 to template nanoparticle surfaces. Our results confirmed that form network...
Pattern generation process for growth of magnetite nanoparticles (MNP), using patterns octadecane thiol and poly(ethylene glycol) to selectively immobilize the biomineralization protein Mms6 form on immobilized Mms6.
Ninhydrin-based fluorometric quantification of phenylalanine is one the most widely used methods for hyperphenylalaninemia (HPA) screening in neonates due to its high sensitivity, accuracy, and low cost. Here we report an increase false positive cases neonatal HPA with this method, caused by contamination blood specimen collection devices during printing process. Through multiple steps verification, contaminants were identified from ink circles printed on indicate positions sizes drops....
Magnetosomes of magnetotactic bacteria consist magnetic nanocrystals with defined morphologies enclosed in vesicles originated from cytoplasmic membrane invaginations. Although many proteins are involved creating magnetosomes, a single magnetosome protein, Mms6, can direct the crystallization magnetite nanoparticles vitro. The vivo role Mms6 formation is debated and observation that binds ferric not ferrous iron raises question how could promote magnetite, which contains both iron. Here we...