A5051458400- Neonatal Health and Biochemistry
- Heme Oxygenase-1 and Carbon Monoxide
- Marine and coastal ecosystems
- Hemoglobin structure and function
- Connective tissue disorders research
- Diatoms and Algae Research
- Marine Biology and Ecology Research
- Antimicrobial Peptides and Activities
- Electrospun Nanofibers in Biomedical Applications
- Biochemical and Structural Characterization
- Phase Equilibria and Thermodynamics
- Erythrocyte Function and Pathophysiology
- Polymer Foaming and Composites
- Drug Transport and Resistance Mechanisms
- Microbial Community Ecology and Physiology
- Marine and coastal plant biology
- Silk-based biomaterials and applications
- Marine Bivalve and Aquaculture Studies
- Legume Nitrogen Fixing Symbiosis
- Air Quality and Health Impacts
- Protist diversity and phylogeny
- Cystic Fibrosis Research Advances
- Liver Disease Diagnosis and Treatment
- Marine Toxins and Detection Methods
- Protein Hydrolysis and Bioactive Peptides
University of Trieste
2010-2025
The effect of P- and N-depletion on the production molecular-level composition extracellular carbohydrates released by diatom Cylindrotheca fusiformis was examined in axenic batch cultures. Inorganic phosphorus depletion caused a higher release organic carbon per cell compared to nutrient-replete nitrogen-limited conditions. Gas-chromatographic analysis monosaccharide dissolved fraction showed galactose be major component exopolymers (33 – 42%). In P-limited treatment, an increase content...
Uveal melanoma (UM) is an ocular cancer, with propensity for lethal liver metastases. When metastatic UM (MUM) occurs, as few 8% of patients survive beyond two years. Efficacious treatments MUM are urgently needed. 1,4-dihydroxy quininib, a cysteinyl leukotriene receptor 1 (CysLT
An elastin-like fusion of the difficult-to-synthesise human β-defensin 1 domain was produced retaining antimicrobial properties peptide. The new biopolymer used to produce coatings and hydrogel-like matrices that promoted cell adhesion.
Two synthetic genes that code for artificial proteins have been constructed were modeled on the most regularly repeated hydrophobic domain of human tropoelastin. We compare physicochemical properties recombinant products differ in their primary structure; alanine/lysine-rich cross-linking domains, which are highly conserved mammalian tropoelastin, either present or absent products. Both biopolymers showed thermoresponsive properties, and variations observed dependent solution conditions....
Bilirubin is a toxicological biomarker for hemolysis and liver diseases. The current automated diazo method used in clinical chemistry has limited applicability rodent models cannot be small animals relevant to toxicology, microphysiological systems, cell cultures, kinetic studies. Here, we present versatile fluorometric nanoscale analysis of bilirubin based on its highly specific binding the recombinant bifunctional protein HELP–UnaG (HUG). assay sensitive (LoQ = 1.1 nM), accurate (4.5%...
The recombinant fusion protein HELP-UnaG (HUG) is a bifunctional product that exhibits human elastin-like polypeptide (HELP)-specific thermal behavior, defined as reverse phase transition, and UnaG-specific bilirubin-dependent fluorescence emission. HUG provides an interesting model to understand how its two domains influence each other's properties. Turbidimetric, calorimetric, light scattering measurements were used determine different parameters for the temperature transition coacervation...
Genetic engineering allows fine-tuning and controlling protein properties, thus exploiting the new derivatives to obtain novel materials systems with improved capacity actively interact biological systems. The elastin-like polypeptides are tunable recombinant biopolymers that have proven be ideal candidates for realizing bioactive interfaces can They characterized by a thermoresponsive behavior is strictly related their peculiar amino acid sequence. We describe here rational design of...
One of the organ-specific functions liver is excretion bilirubin into bile. Membrane transport from blood to not only an orphan function, because there no link protein/gene units that perform this but also a poorly characterised function. The aim study was investigate pharmacology uptake in female Wistar rat improve basic knowledge neglected area physiology. We treated isolated perfused livers rats with repeated single-pass, albumin-free boli. monitored both and glucuronide perfusion...