A5012522673- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- Wine Industry and Tourism
- Yeasts and Rust Fungi Studies
- Probiotics and Fermented Foods
- Meat and Animal Product Quality
- Fungal Biology and Applications
- Polyamine Metabolism and Applications
- Listeria monocytogenes in Food Safety
- Biochemical Analysis and Sensing Techniques
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Essential Oils and Antimicrobial Activity
- Phytochemicals and Antioxidant Activities
- Plant Molecular Biology Research
- Microbial Metabolism and Applications
- Proteins in Food Systems
- Insect and Pesticide Research
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Catalysis and Immobilization
- Biochemical and biochemical processes
- Bee Products Chemical Analysis
- Heat shock proteins research
- Food Quality and Safety Studies
- Fungal and yeast genetics research
National Agricultural Research Foundation
2011-2024
Agricultural University of Athens
2007-2011
Technological Educational Institute of Athens
2011
University of Reading
2005
The yeast Candida zemplinina (Starmerella bacillaris) is frequently isolated from grape and wine environments. Its enological use in mixed fermentation with Saccharomyces cerevisiae has been extensively investigated these last few years, several interesting features including low ethanol production, fructophily, glycerol other metabolites have described. In addition, molecular tools allowing the characterization of populations developed, both at inter- intraspecific levels. However, most...
Indigenous yeast population dynamics during the fermentation of healthy and Botrytis-affected grape juice samples from two regions in Greece, Attica Arcadia, were surveyed. Species diversity was evaluated by using restriction fragment length polymorphism sequence analyses 5.8S internal transcribed spacer D1/D2 ribosomal DNA (rDNA) cultivable yeasts. Community-level profiles also obtained direct analysis fermenting through denaturing gradient gel electrophoresis 26S rDNA amplicons. Both...
To assess the yeast community structure and dynamics during Greek-style processing of natural black Conservolea olives in different brine solutions.Black were subjected to spontaneous fermentation 6% (w/v) NaCl solution or supplemented with (i) 0.5% glucose, (ii) 0.2% (v/v) lactic acid (iii) both glucose acid. Yeast species diversity was evaluated at early (2 days), middle (17 days) final (35 stages by restriction fragment length polymorphism sequence analyses 5.8S internal transcribed...
The yeast flora associated with healthy and Botrytis-infected grapes was assessed. Molecular identification methods assigned isolates to six genera nine species. For the first time Hanseniaspora opuntiae encountered as an inhabitant of grape ecosystem. By using DraI, informative restriction fragment length polymorphism pattern generated distinguish H. from closely related organism guilliermondii. Botrytis infection resulted in a larger population greater diversity yeasts enriched...
Epigenetics refers to dynamic chemical modifications the genome that can perpetuate gene activity without changes in DNA sequence. Epigenetic mechanisms play important roles growth and development. They may also drive plant adaptation adverse environmental conditions by buffering variation. Grapevine is an perennial fruit crop cultivated worldwide, but mostly temperate zones with hot dry summers. The decrease rainfall rise temperature due climate change, along expansion of pests diseases,...
To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel-derived glycerol (Gly) into added-value compounds.Ten Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, from fishes, metabolized Gly under nitrogen limitation. The aim study was identify potential microbial candidates that could produce single-cell oil (SCO), endopolysaccharides polyols when these micro-organisms were grown on Gly. As...
Lactic acid production is an important feature of the yeast Lachancea thermotolerans that has gained increasing interest in winemaking. In particular, light climate change, biological acidification and ethanol reduction by use selected strains may counteract effect global warming wines. Here, enological potential a high lactate-producing L. strain (P-HO1) mixed fermentations with S. cerevisiae was examined. Among different inoculation schemes evaluated, most successful implantation...
Recent research has expanded our understanding on vineyard-associated fungal community assembly, suggesting non-random distribution and implicating regional differences in the wine terroir effect. Here, we focused culturable fraction of that resides grapes determine quality, so-called yeast populations. We aimed to analyze local-scale assemblages test whether hypothesis biogeographical patterns also applies yeasts particular. Surveying 34 vineyards across four main viticultural zones Greece...
In view of climate change and the increasingly antagonistic wine market, exploitation native genetic resources is revisited in relation to sustainable production. ‘Sideritis’ a late-ripening Greek grape variety, which quite promising for counteracting quality issues associated with annual temperature rise. The aim this study was improve enhance aroma through use yeasts. To vinification, Hanseniaspora opuntiae L1 used along Saccharomyces cerevisiae W7 mixed fermentations (SQ). addition H....
Multi-starter wine fermentations employing non-Saccharomyces (NS) yeasts are becoming an emerging trend in winemaking. It is therefore important to determine the impacts of different NS strains phenotype and particular aroma outputs inoculation schemes fermentation conditions. Here, two native yeasts, Lachancea thermotolerans LtMM7 Hanseniaspora uvarum HuMM19, were assessed for their ability improve quality Moschofilero, a Greek aromatic white wine. The initially examined laboratory scale...
Non-Saccharomyces (NS) yeasts are gaining popularity in modern winemaking for improving wine quality. Climate change is one of the biggest challenges winegrowing now faces warm regions. Here, Lachancea thermotolerans LtS1 and Torulaspora delbrueckii TdS6 combined with Saccharomyces cerevisiae ScS13 isolated from Assyrtiko grapes Santorini island were evaluated grape must fermentation aim to mitigate major consequences temperature rise. Different inoculation protocols evaluated, including...
Abstract Background and Aims: Heat shock protein 90 (Hsp90) proteins constitute an important gene family of molecular chaperons. High-temperature stress, which is often combined with drought may exert major constraints to grapevine growth development. The aim this study was characterise the Vitis vinifera Hsp90 family. Methods Results: Using complete genome sequences, four cytoplasmic three organelle-specific V. (VvHsp90) were identified. Phylogenetic analysis revealed that they share high...
Two bacterial strains (B18BM42 T and B18NM6) were recovered during a study of diversity on wine grapes ( Vitis vinifera L.) from the Nemea region in Greece. Phylogenetic analysis based 16S rRNA gene sequences placed two within genus Weissella , found them to be most closely related minor NRIC 1625 followed by viridescens 1536 (99.1 98.9 % sequence similarity, respectively). The level DNA–DNA relatedness between B18NM42 W. or was 31.9 35.0 %, respectively. novel could genetically...
Vineyard- and winery-associated lactic acid bacteria (LAB) from two major PDO regions in Greece, Peza Nemea, were surveyed. LAB isolated grapes, fermenting musts, winery tanks performing spontaneous malolactic fermentations (MLF). Higher population density species richness detected Nemea than vineyards on grapes musts. Pediococcus pentosaceus Lactobacillus graminis the most abundant while plantarum dominated musts both regions. No particular structure of populations according to region...
Four ascomycetous yeast strains (D4W13, D9W2, D9W4 and D9W17T) were isolated from Botrytis-affected fermenting grape juice originating Attica Province, Greece. Phylogenetic analysis of rRNA gene sequences (18S, 26S 5.8S-ITS) showed that the four represent a distinct species within genus Kazachstania, closely related to Kazachstania zonata NBRC 100504T gamospora 11056T. Electrophoretic karyotyping physiological support affiliation in novel for which name hellenica sp. nov. is proposed, with...